Sort1 (NM_001271599) Mouse Untagged Clone

CAT#: MC229056

Sort1 (untagged) - Mouse sortilin 1 (Sort1), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Sort1
• Synonyms: 2900053A11Rik; AI852375; Ntr3; Ntsr3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229056 representing NM_001271599
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCGGCCCCGGGGAGCTGCGGACGGCCTTTTGCGCTGGCCCCTCGGCCTCCTCCTGCTCCTTCAAC
  TGCTGCCTCCTGCCGCCGTCGGCCAGGACCGGCTGGACGCGCCGCCGCCGCCCGCGCCTCCTCTGCTGCG
  CTGGGCCGGTCCGGTCGGGGTGAGCTGGGGGCTGCGCGCCGCCGCGCCCGGGGGCCCCGTCCCCCGCGCT
  GGCCGTTGGCGCCGCGGCGCGCCCGCCGAGGACCAAGACTGCGGCCGCCTCCCGGACTTCATCGCCAAGC
  TGACCAACAATACGCACCAGCATGTCTTTGATGACCTCAGTGGCTCAGTGTCCTTGTCCTGGGTTGGAGA
  CAGCACTGGGGTTATTCTCGTCCTGACCACTTTCCAAGTGCCTCTGGTAATTGTGAGCTTTGGACAGTCC
  AAGTTGTATCGAAGTGAGGATTATGGAAAGAACTTTAAGGATATTACAAATCTCATCAATAACACCTTCA
  TTCGGACGGAATTTGGCATGGCTATTGGTCCTGAGAACTCTGGAAAGGTGATACTAACAGCGGAGGTGTC
  CGGGGGAAGCCGAGGCGGAAGAGTGTTCAGGTCATCAGACTTTGCCAAGAACTTTGTGCAAACAGATCTC
  CCCTTTCATCCTCTGACGCAGATGATGTACAGCCCTCAGAATTCTGATTACCTGTTAGCTCTCAGCACCG
  AAAATGGCCTGTGGGTGTCCAAGAATTTTGGGGAAAAATGGGAAGAAATCCACAAAGCAGTATGTTTGGC
  CAAATGGGGACCAAACAACATCATCTTCTTTACCACCCATGTGAATGGCTCCTGCAAAGCTGATCTTGGT
  GCCCTGGAATTATGGAGAACATCCGACTTGGGAAAAACCTTCAAAACCATTGGTGTGAAAATCTACTCCT
  TTGGTCTTGGGGGCCGTTTCCTTTTTGCCTCTGTGATGGCTGATAAGGACACAACAAGAAGGATCCATGT
  GTCAACAGACCAGGGGGACACATGGAGCATGGCACAACTTCCTTCTGTGGGACAGGAACAGTTCTACTCC
  ATCCTGGCAGCCAATGAGGACATGGTCTTCATGCATGTAGATGAACCTGGAGATACCGGGTTTGGCACCA
  TCTTTACCTCTGATGATCGAGGCATTGTCTACTCCAAGTCTCTGGACAGACATCTCTATACCACCACAGG
  CGGGGAGACGGACTTTACCAACGTGACTTCCCTCCGTGGGGTCTATATAACAAGCACGCTCTCAGAAGAT
  AACTCTATTCAGAGCATGATCACTTTTGACCAGGGAGGACGGTGGGAGCACCTGCGGAAGCCGGAGAACA
  GCAAGTGCGACGCTACCGCAAAGAACAAGAACGAGTGCAGCCTTCATATCCATGCTTCTTATAGCATCTC
  CCAGAAGCTAAACGTTCCAATGGCCCCACTTTCCGAGCCCAATGCTGTGGGCATAGTCATCGCTCACGGT
  AGTGTGGGAGATGCCATCTCGGTGATGGTCCCAGATGTGTACATCTCAGATGATGGGGGTTACTCCTGGG
  CGAAGATGCTAGAAGGACCACATTACTATACCATCCTGGACTCTGGAGGCATCATTGTGGCCATTGAGCA
  CAGCAACCGTCCTATCAATGTGATTAAGTTCTCCACAGATGAAGGCCAGTGCTGGCAGAGCTATGTGTTC
  ACACAGGAGCCCATCTACTTCACTGGGCTTGCTTCCGAGCCTGGAGCCAGGTCCATGAACATCAGCATCT
  GGGGATTCACAGAGTCTTTCATTACCCGCCAGTGGGTCTCCTACACAGTCGATTTCAAAGACATCCTTGA
  GCGGAATTGTGAAGAGGATGACTATACCACGTGGCTGGCACACTCCACAGACCCTGGAGATTACAAAGAC
  GGCTGCATTTTGGGCTATAAAGAACAGTTCCTACGGCTACGGAAGTCATCCGTCTGTCAGAATGGTCGAG
  ACTATGTTGTGGCCAAGCAGCCATCCGTCTGTCCGTGTTCCCTGGAGGACTTCCTCTGTGACTTTGGCTA
  CTTCCGTCCGGAGAACGCCTCAGAGTGCGTGGAGCAGCCTGAACTGAAGGGGCATGAGTTAGAGTTCTGT
  CTGTACGGCAAGGAGGAGCACCTGACAACAAATGGGTACCGGAAAATCCCAGGAGACAAATGCCAAGGTG
  GGATGAATCCCGCCAGAGAAGTAAAAGACTTGAAAAAGAAATGCACAAGCAACTTCTTGAACCCCACAAA
  GCAGGACTCCCGCCCACAGGGACACAGCTTGTCCCAGAATCCAGCTCCGCCTCCTCTTGGATACACTGAA
  AACACACACTTCCTATCTCCTACCCAGAAGCAGAATTCCAAGTCAAATTCTGTCCCTATTATCCTGGCCA
  TCGTGGGACTGATGCTTGTCACAGTCGTAGCAGGAGTCCTCATTGTGAAGAAATATGTCTGTGGCGGAAG
  GTTCCTGGTGCACCGGTACTCGGTGCTACAGCAGCACGCAGAGGCTGACGGCGTAGAGGCTTTGGATTCA
  ACCTCCCACGCTAAAAGCGGATATCACGACGACTCAGATGAGGACCTCCTGGAATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001271599
• Insert Size: 2577 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001271599.1, NP_001258528.1
• RefSeq Size: 6950 bp
• RefSeq ORF: 2577 bp
• Locus ID: 20661
• UniProt ID: Q6PHU5
• Cytogenetics: 3 F3
• Gene Summary: Functions as a sorting receptor in the Golgi compartment and as a clearance receptor on the cell surface. Required for protein transport from the Golgi apparatus to the lysosomes by a pathway that is independent of the mannose-6-phosphate receptor (M6PR). Also required for protein transport from the Golgi apparatus to the endosomes. Promotes neuronal apoptosis by mediating endocytosis of the proapoptotic precursor forms of BDNF (proBDNF) and NGFB (proNGFB). Also acts as a receptor for neurotensin. May promote mineralization of the extracellular matrix during osteogenic differentiation by scavenging extracellular LPL. Probably required in adipocytes for the formation of specialized storage vesicles containing the glucose transporter SLC2A4/GLUT4 (GLUT4 storage vesicles, or GSVs). These vesicles provide a stable pool of SLC2A4 and confer increased responsiveness to insulin. May also mediate transport from the endoplasmic reticulum to the Golgi.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).