Sgip1 (NM_001285852) Mouse Untagged Clone

CAT#: MC229051

Sgip1 (untagged) - Mouse SH3-domain GRB2-like (endophilin) interacting protein 1 (Sgip1), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Sgip1
• Synonyms: 3110007P09Rik; gag; SGIP1alpha
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229051 representing NM_001285852
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGGAAGGACTGAAAAAACGTACAAGGAAGGCCTTTGGAATACGGAAGAAAGAAAAAGACACTGACT
  CTACAGGCTCGCCAGACCGAGATGGAATGCAGGGGAAAAAAAAGGCTCAGAAGACACAGCTTCTCCTCAC
  CTCTTGCTTCTGGCTCAGAGCCCTCTCCTTAACTCTGTCTCAGCAGCCCAGCCCACACGAGCCACCCTAC
  CATAGCAAAGCAGAGTGTGCGCGGGAAGGAGGGAAGAAAGCTTCGAAGAAAAGCAATGGGGCACCAAATG
  GATTTTATGCAGAAATCGATTGGGAAAGATATAACTCACCTGAGCTAGATGAAGAAGGTTACAGCATCAG
  ACCTGAGGAACCAGGCTCTACCAAAGGAAAGCACTTTTATTCCTCAAGTGAATCTGAAGAGGAAGAAGAG
  TCGCACAAGAAATTCAACATCAAGATTAAACCCTTGCAATCTAAGGACGTCCTGAAGAATGCAGCGACGG
  TAGATGAGCTGAAGGCTTCAATAGGCAACATCGCACTTTCCCCTTCGCCTGTGAGGAAAAGTCCGAGGCG
  CAGCCCGGGTGCAATTAAAAGGAACTTATCCAGTGAAGAAGTTGCAAGACCCAGGCGTTCCACCCCAACT
  CCAGAACTTACAAGCAAGAAGCCTCTGGATGACACTCTGGCCCTTGCTCCCCTCTTTGGTCCACCATTAG
  AGTCAGCTTTTGATGAGCAGAAGACGGAAGTTCTTTTAGATCAGCCTGAGATATGGGGTTCAGGCCAACC
  GATGAACCCAAGTACGGAGTCGCCAGAGCTAGCAAGACCTTTCCCCACTGGAACACCTCCACCTCTGCCT
  CCAAAAACTGTACCAGCCACTCCGCCTCGAACAGGCTCCCCCTTAACAGTTGCAACAGGAAATGACCAGG
  CAGCCACAGAGGCCAAAATTGAGAAACTACCATCCATCAGTGACCTGGACAGCATTTTTGGGCCAGTGTT
  GTCGCCCAAGTCTGTTGCTGTTAATACTGAAGAGAAGTGGGTCCATTTTTCTGATGCATCCCCGGAACAT
  GTTACTCCAGAGTTGACTCCAAGGGAACAGGTGGTGACCCCACCAGCTGCATCAGACATCCCAGCTGACT
  CCCCAGCTCCAGCCCCACCTGGCCCCACAGGCTCCGCAGGTCCCCCAGGGCCTCCTGGTCCTCGCCATGT
  ACCATCTCCGCTCAATTTAGAAGAAGTCCAGAAGAAAGTTGCTGAGCAGACCTTCATTAAAGATGATTAC
  TTAGAAACACTCTCATCTCCTAAAGAGTGTGGGTTGGGACAGAGAGCAACTCCACCTCCCCCACCACCAC
  CCACCTACAGGACTGTGGTCTCGTCCCCCGGACCTGGCTCGGGCAGTGGTACGGGGACCACCAGTGGTGC
  GTCTTCTCCTGCTCGACCAGCCACCCCCTTAGTTCCTTGCAGCACCACTCCGCCTCCACCTCCTCCCCGG
  CCTCCATCCCGGCCAAAGCTACCTCCAGGAAAGCCTGGAGTTGGAGATGTGTCCAGACCTTTTAGCCCAC
  CCATACACTCCTCCAGCCCTCCTCCGATAGCACCCTTAGCCCGGGCTGAAAGCACTTCTTCAATATCGTC
  AACTAACTCACTGAGCGCAGCCACCACTCCCACAGTTGAGAATGAACAGCCCTCCCTCGTTTGGTTTGAC
  AGAGGAAAGTTTTATTTGACTTTTGAAGGTTCCTCCCGGGGGCCCAGTCCCCTAACTATGGGGGCCCAGG
  ACACCCTCCCGGTCGCAGCAGCTTTCACAGAAACTGTCAACGCCTACTTCAAAGGAGCAGATCCAAGCAA
  GTGCATTGTTAAGATCACGGGAGAAATGGTGTTGTCCTTCCCTGCTGGTATCACCAGACACTTTGCCAAC
  AACCCATCCCCAGCAGCTCTGACTTTCCGGGTGGTAAATTCCAGCAGGTTAGAGCACGTCCTGCCGAACC
  CCCAACTTCTCTGCTGTGATAATACACAAAATGATGCCAATACCAAGGAATTCTGGGTAAACATGCCAAA
  TTTGATGACTCACCTAAAGAAAGTCTCTGAACAAAAACCCCAGGCTACATACTATAATGTGGACATGCTC
  AAATATCAGGTGTCTGCCCAGGGCATTCAGTCCACACCTCTGAACTTGGCGGTGAACTGGCGCTGTGAGC
  CTGCGAGCACTGACCTGCGCATAGACTATAAGTACAACACGGATGCCATGTCCACTGCAGTGGCCCTCAA
  CAACGTGCAGTTCCTGGTCCCCATAGACGGAGGAGTGACCAAGCTTCAGGCTGTGCTTCCTCCAGCAGTC
  TGGAATGCTGAACAACAAAGAATATTATGGAAGATTCCCGATATCTCCCAGAAGTCAGAAAATGGAGGCG
  TAGGTTCTTTACTGGCGAGATTTCAGTTATCCGAAGGCCCGAGCAAACCTTCCCCGCTGGTTGTGCAGTT
  CACGAGTGAAGGAAGTACTCTCTCTGGCTGTGACATTGAACTTGTTGGAGCAGGCTACCGGTTTTCACTC
  ATTAAGAAGAGGTTTGCTGCAGGAAAGTACTTGGCAGATAACTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001285852
• Insert Size: 2565 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001285852.1, NP_001272781.1
• RefSeq Size: 5589 bp
• RefSeq ORF: 2565 bp
• Locus ID: 73094
• Cytogenetics: 4 C6
• Gene Summary: May function in clathrin-mediated endocytosis. Has both a membrane binding/tubulating activity and the ability to recruit proteins essential to the formation of functional clathrin-coated pits. Has a preference for membranes enriched in phosphatidylserine and phosphoinositides and is required for the endocytosis of the transferrin receptor. May also bind tubulin. May play a role in the regulation of energy homeostasis.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.