Trpc6 (NM_001282086) Mouse Untagged Clone

CAT#: MC229048

Trpc6 (untagged) - Mouse transient receptor potential cation channel, subfamily C, member 6 (Trpc6), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Trpc6
• Synonyms: AV025995; LLHWJM002; LLHWJM003; LLHWJM004; mtrp6; TRP-6; Trrp6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229048 representing NM_001282086
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCCAGAGCCCGAGGTTCGTGACCCGGAGGGGCGGCTCTCTAAAGGCTGCCCCTGGAGCCGGCACCC
  GGCGCAACGAGAGCCAGGACTATTTGCTGATGGACGAGCTGGGAGACGACGGCTACCCGCAGCTCCCGCT
  GCCACCGTATGGCTACTACCCCAGCTTCCGGGGTAATGAAAACAGACTGACTCACCGGCGGCAGACGATT
  CTTCGTGAGAAGGGAAGAAGGTTAGCTAATCGAGGACCAGCATACATGTTTAATGATCATTCAACAAGCC
  TGTCTATTGAGGAAGAACGCTTTCTAGATGCAGCTGAATATGGCAACATCCCAGTGGTGCGGAAGATGCT
  AGAAGAGTGTCATTCCCTCAATGTTAACTGTGTGGATTACATGGGCCAGAATGCCCTACAGCTGGCTGTG
  GCCAATGAGCACTTGGAAATCACAGAGCTGCTACTCAAGAAGGAAAACTTGTCTCGAGTTGGGGATGCTT
  TACTTTTAGCCATTAGTAAAGGTTATGTACGGATTGTGGAGGCAATCCTCAACCATCCAGCTTTTGCTGA
  AGGCAAAAGGTTAGCGACAAGCCCCAGCCAGTCTGAACTTCAGCAAGATGACTTTTATGCCTATGATGAA
  GATGGGACGCGGTTCTCCCATGATGTGACCCCAATCATTCTCGCTGCACATTGCCAGGAATATGAAATTG
  TGCATACCCTCCTGAGAAAGGGTGCCCGGATTGAGCGGCCTCATGATTACTTCTGCAAGTGTACAGAATG
  CAGCCAGAAGCAGAAGCATGATTCCTTCAGCCACTCTAGATCCAGGATCAATGCATACAAAGGTCTGGCA
  AGTCCAGCATACCTGTCATTGTCCAGTGAAGATCCAGTCATGACTGCTTTAGAACTTAGCAATGAGCTGG
  CAGTGCTTGCCAACATTGAGAAAGAGTTCAAGAATGACTACAGGAAGCTGTCTATGCAGTGCAAGGATTT
  CGTTGTTGGTCTCTTGGACCTCTGCAGAAACACAGAGGAAGTGGAGGCCATCCTGAATGGGGATGCAGAG
  ACTCGCCAGCCCGGGGACTTCGGCCGTCCAAATCTCAGCCGTTTAAAACTTGCTATTAAGTATGAAGTAA
  AAAAATTTGTGGCTCATCCAAACTGTCAGCAACAGCTCCTGTCCATATGGTATGAGAACCTCTCTGGTTT
  ACGGCAGCAGACCATGGCAGTGAAGTTCCTCGTGGTCCTTGCTGTTGCCATTGGATTGCCCTTCCTGGCT
  CTCATATACTGGTGTGCTCCTTGCAGCAAGATGGGGAAGATATTGCGAGGACCGTTCATGAAGTTTGTAG
  CACACGCAGCCTCCTTCACCATTTTCCTGGGGCTGCTCGTCATGAATGCAGCTGACAGATTTGAAGGCAC
  CAAGCTCCTCCCTAATGAAACCAGCACAGATAATGCAAGGCAGCTGTTCAGGATGAAAACATCCTGTTTC
  TCATGGATGGAGATGCTCATTATATCCTGGGTAATAGCCAGGATAAAGTGGGACCCTACTGATCCTCAGA
  TCATCTCTGAAGGTCTTTATGCAATCGCTGTGGTTTTAAGTTTCTCCAGAATAGCTTACATTTTACCAGC
  AAATGAAAGCTTTGGACCTCTGCAGATTTCACTTGGAAGAACAGTGAAAGATATCTTCAAATTCATGGTC
  ATATTCATCATGGTGTTTGTAGCCTTTATGATTGGAATGTTCAACCTTTACTCCTACTACATTGGCGCAA
  AACAGAATGAAGCATTCACAACAGTTGAGGAAAGTTTTAAGACACTGTTCTGGGCTATCTTTGGTCTTTC
  TGAAGTGAAGTCAGTGGTCATTAACTACAATCACAAGTTCATTGAAAACATCGGCTACGTTCTGTATGGT
  GTCTATAATGTCACAATGGTCATTGTTTTGCTAAATATGTTAATTGCGATGATCAATAGTTCATTCCAGG
  AAATTGAGGATGATGCGGACGTGGAGTGGAAGTTTGCAAGGGCCAAATTGTGGTTTTCCTACTTTGAGGA
  GGGGAGAACACTTCCTGTCCCCTTCAATCTTGTACCAAGTCCAAAATCCTTGCTTTATCTCCTATTGAAA
  TTTAAGAAATGGATGTGTGAGCTCATCCAGGGTCAAAAGCAAGGCTTCCAAGAAGATGCAGAGATGAACA
  AGAGAAATGAAGAAAAGAAATTTGGAATTTCAGGAAGTCACGAAGACCTTTCAAAATTTTCACTTGACAA
  AAATCAGTTGGCACACAACAAACAATCAAGTACAAGGAGCTCAGAAGATTATCATTTAAATAGTTTCAGT
  AACCCTCCAAGACAATATCAGAAAATCATGAAGAGACTCATTAAAAGATATGTATTGCAGGCCCAGATTG
  ATAAGGAGAGCGATGAGGTGAATGAAGGGGAATTGAAGGAAATTAAGCAAGACATCTCAAGTCTCCGTTA
  TGAACTCCTTGAAGAGAAATCACAGAACACAGAAGACCTAGCAGAGCTCATTAGAAAACTCGGGGAGAGA
  CTGTCGTTAGAGCCAAAGCTGGAGGAAAGCCGCAGATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001282086
• Insert Size: 2559 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001282086.1, NP_001269015.1
• RefSeq Size: 3025 bp
• RefSeq ORF: 2559 bp
• Locus ID: 22068
• Cytogenetics: 9 2.46 cM
• Gene Summary: Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C. Seems not to be activated by intracellular calcium store depletion.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an in-frame exon in the central coding region, compared to variant 1. The encoded isoform (2) is shorter, compared to isoform 1.