Sun1 (NM_001256116) Mouse Untagged Clone

CAT#: MC229043

Sun1 (untagged) - Mouse Sad1 and UNC84 domain containing 1 (Sun1), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Sun1
• Synonyms: 4632417G13Rik; 5730434D03Rik; mKIAA0810; Unc84a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229043 representing NM_001256116
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACTTTTCTCGGCTGCACACGTACACCCCACCCCAGTGTGTGCCGGAGAACACTGGCTACACTTACG
  CACTCAGTTCTAGTTACTCGTCGGATGCTCTGGATTTTGAAACTGAGCACAAGTTGGAACCTGTATTTGA
  CTCTCCAAGGATGTCCCGCCGCAGCTTGCGTCTGGTCACAACAGCTTCGTACAGCAGTGGGGACAGCCAG
  GCTATTGATTCGCACATTAGCACCAGCAGGGCCACCCCCGCCAAGGGGAGAGAAACCAGGACAGTCAAAC
  AGAGAAGAAGTGCAAGCAAGCCAGCTTTTAGTATCAACCACCTGTCAGGGAAGGGCTTGTCCTCGAGCAC
  AAGCCATGACAGCTCTTGCAGCCTGCGGAGTGCCACGGTGCTGCGGCACCCTGTGCTAGATGAGTCCCTG
  ATTCGTGAGCAGACCAAAGTGGACCACTTCTGGGGTCTCGATGATGATGGTGACCTTAAAGGTGGAAATA
  AAGCTGCCACTCAGGGAAATGGTGAACTGGCAGCAGAGGTGGCGAGCAGCAATGGATACACTTGCCGTGA
  CTGCAGGATGCTCTCAGCGCGCACTGACGCACTCACAGCCCACTCTGCCATCCACGGGACCACCTCCAGG
  GTGTACTCCAGAGACAGGACTCTCAAACCACCCCATCTCGGTCACTGTGGGAGGATGACTGCCGGAGAAC
  TTTCCAGAGTGGACGGGGAGTCCCTGTGCGATGACTGTAAGGGGAAGAAGCACCTTGAGATACACACAGC
  CACCCACTCGCAACTGCCCCAGCCACACAGGGTGGCCGGGGCCATGGGGCGCCTCTGCATCTATACAGGT
  GACCTCTTGGTTCAAGCACTGCGAAGGACTAGAGCTGCCGGGTGGTCTGTGGCCGAGGCCGTGTGGTCGG
  TGCTCTGGCTGGCTGTCTCTGCTCCAGGGAAGGCAGCCTCGGGAACCTTCTGGTGGCTAGGGAGCGGCTG
  GTACCAATTTGTTACTTTGATTTCTTGGCTGAATGTCTTTCTTCTTACCAGGTGCCTTCGAAATATTTGC
  AAGGTTTTTGTCTTGCTCCTCCCACTCCTACTTTTACTAGGTGCTGGTGTCTCCCTGTGGGGCCAGGGAA
  ACTTCTTCTCACTCCTACCAGTGCTGAACTGGACGGCCATGCAGCCAACACAGAGGGTGGACGATTCCAA
  GGGCATGCATAGACCTGGCCCTCTTCCCCCGAGCCCACCTCCAAAGGTTGATCACAAGGCTTCCCAGTGG
  CCTCAGGAGAGTGACATGGGGCAGAAGGTAGCTTCTTTGAGTGCGCAGTGCCACAACCATGATGAGAGAC
  TTGCAGAGCTGACAGTCCTGCTTCAGAAACTACAGATACGGGTAGACCAAGTGGATGACGGCAGGGAAGG
  GCTGTCACTGTGGGTCAAGAATGTGGTTGGACAGCACCTGCAGGAGATGGGCACCATAGAACCACCTGAT
  GCTAAGACTGACTTCATGACTTTCCACCATGACCATGAAGTGCGTCTCTCCAACTTGGAAGATGTTCTTA
  GAAAACTGACAGAAAAATCTGAGGCTATCCAGAAGGAGCTGGAAGAAACCAAGCTGAAAGCAGGCAGCAG
  GGATGAAGAGCAGCCCCTCCTTGACCGTGTGCAGCACCTAGAACTGGAACTGAACCTGTTGAAGTCACAG
  CTGTCAGACTGGCAGCATCTGAAGACCAGCTGTGAGCAGGCTGGGGCCCGCATCCAGGAGACTGTGCAGC
  TCATGTTCTCTGAGGATCAGCAGGGCGGTTCCCTCGAGTGGCTATTAGAGAAGCTTTCTTCTCGGTTCGT
  GAGCAAGGATGAGCTGCAGGTGCTCTTACATGACCTTGAGCTGAAACTGCTGCAGAATATCACACACCAC
  ATCACCGTGACAGGACAGGCCCCGACATCCGAGGCTATTGTGTCTGCCGTGAATCAGGCAGGGATTTCAG
  GAATCACAGAAGCGCAAGCACATATCATTGTGAACAATGCTCTGAAGCTGTACTCCCAAGACAAGACGGG
  GATGGTGGACTTTGCTCTGGAGTCTGGAGGTGGCAGCATCCTAAGCACTCGGTGCTCTGAGACCTATGAG
  ACCAAGACGGCACTGCTGAGCCTGTTTGGGGTCCCACTGTGGTACTTCTCACAGTCACCTCGAGTGGTGA
  TCCAGCCCGACATCTACCCAGGGAATTGCTGGGCGTTCAAAGGTTCCCAGGGGTACCTGGTGGTGCGGTT
  GTCCATGAAGATCTACCCAACCACATTCACCATGGAACACATTCCAAAGACACTATCACCCACTGGTAAC
  ATCTCCAGTGCCCCCAAAGACTTTGCAGTCTATGGACTGGAAACGGAGTATCAAGAAGAGGGGCAGCCTC
  TGGGACGGTTCACCTATGACCAGGAAGGAGACTCACTCCAGATGTTCCACACACTGGAAAGACCTGACCA
  AGCCTTCCAGATAGTAGAGCTCCGGGTCCTGTCCAACTGGGGCCACCCTGAGTACACTTGCCTCTACCGG
  TTCCGAGTCCACGGAGAGCCCATCCAGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001256116
• Insert Size: 2550 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001256116.1, NP_001243045.1
• RefSeq Size: 3878 bp
• RefSeq ORF: 2550 bp
• Locus ID: 77053
• UniProt ID: Q9D666
• Cytogenetics: 5 G2
• Gene Summary: As a component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex involved in the connection between the nuclear lamina and the cytoskeleton (PubMed:20711465, PubMed:16380439, PubMed:24062341, PubMed:25892231, PubMed:26842404). The nucleocytoplasmic interactions established by the LINC complex play an important role in the transmission of mechanical forces across the nuclear envelope and in nuclear movement and positioning (PubMed:19874786). Required for interkinetic nuclear migration (INM) and essential for nucleokinesis and centrosome-nucleus coupling during radial neuronal migration in the cerebral cortex and during glial migration (PubMed:19874786). Involved in telomere attachment to nuclear envelope in the prophase of meiosis implicating a SUN1/2:KASH5 LINC complex in which SUN1 and SUN2 seem to act at least partial redundantly (PubMed:17543860, PubMed:19211677, PubMed:19509342, PubMed:24062341, PubMed:25892231, PubMed:26842404). Required for gametogenesis and involved in selective gene expression of coding and non-coding RNAs needed for gametogenesis (PubMed:17543860). Helps to define the distribution of nuclear pore complexes (NPCs) (PubMed:17724119). Required for efficient localization of SYNE4 in the nuclear envelope (PubMed:23348741). May be involved in nuclear remodeling during sperm head formation in spermatogenenis (PubMed:20711465). May play a role in DNA repair by suppressing non-homologous end joining repair to facilitate the repair of DNA cross-links (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) lacks an in-frame exon in the coding region, compared to variant 1. The resulting isoform (3) is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.