Ano7 (NM_001271884) Mouse Untagged Clone

CAT#: MC229033

Ano7 (untagged) - Mouse anoctamin 7 (Ano7), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ano7
• Synonyms: IPC; NG; NGE; Ngep; Ngep-L; Pc; Pcanap5; Tmem16g
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229033 representing NM_001271884
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCGGGGGCAAGCGCGAGAAGAAGACAGCGTGGTGCTGATCGACATGGCCTCCCCTGAAGCAGGGA
  ATGGATGCTCCTATGGGAGCACAGCCCAAGCCTCAGAGGCAGGTAAACAGCAGGTGGCCCCCAGCAGAGT
  TGGGAGCTCTGCCAAACCCCCAATTGATTTTGTTCTTGTTTGGGAAGAAGACCTGAGGAACCAAGAGAAC
  CCCACGAAGGACAAGACAGACACACACGAGGTCTGGCGGGAGACTTTTCTGGAGAATCTTTGCTTGGCTG
  GCCTGAAAATAGATCAGCATGATGTCCAGGATGAGGCCGCTGCAGTTCATTACATCCTCCTCCGCGCACC
  CTGGGCTGTGCTTTGCTACTATGCAGAAGACCTGCGCCTGAAGCTGCCTCTACAGGAGTTACCCAACCAG
  GCCTCCAACTGGTCAGCCACCCTTCTGGAGTGGCTGGGCATCCCCAATATCCTGCTGGAGCACGTGCCTG
  ACACGCCCCCCGAGTATTACTCCTGCCAGTTCAAAGCAAGCAAGCTGCAATGGTTTCTTGGGAGTGACAA
  CCAAGACACCTTCTTCACCAGCACCAAGAGGCATCAGATTCTATTTGAGATCCTGGCCAAGACTCCATAT
  GGGCACGAGAAGAAAGGCCTGTTTGGGATTGACCAGCTGCTGGCAGAAGGTGTATTCAGTGCAGCCTTCC
  CTCTGCATGACGGCCCATTCTCTGCAGTCCCAGAGAGCTCACAGGTCCTGGGTCTCATCCAACGCCAAGT
  CTTATTCCAGCACTGGGCTCGCTGGGGCAAGTGGAACAAGTACCAGCCATTGGACCATGTGCGAAGGTAC
  TTCGGGGAGAAGGTGGCTCTCTACTTCGCCTGGCTTGGGTTTTACACTGGTTGGCTCCTGCCTGCGGCAG
  TAGTGGGCACAGTGGTCTTCCTGGTGGGATGTTTCCTGGTGTTCTCAGACATACCAACGCAGGAGCTGTG
  TCACAGCTCAGACAGCTTCGACATGTGCCCGCTGTGCTCCGACTGTTCTTTTTGGCTGCTCTCCAGTGCC
  TGCACCCTGGCCCAGGCTGGGCGGCTCTTCGACCATGGCGGCACCGTCTTCTTCAGTTTGTTCATGGCAC
  TGTGGGCGGTGCTGCTTCTGGAGTACTGGAAGCGGAAAAACGCCACATTGGCCTACCGCTGGGACTGTTC
  TGACTATGAAGACATTGAGGAGAGGCCTCGGCCCCAGTTCGCTGCAACGGCCCCCATGACAGCCCTGAAC
  CCCATCACGGGGGAAGATGAGCCCTACTTTCCTGAGAAGAATCGTGTACGCCGCATGCTGGCTGGTTCTG
  TGGTGCTCTTGATGATGGTGGCTGTGGTGATTATGTGCCTTGTATCTGTCATCCTGTACCGGGCCGTCAT
  GGCTATCATTGTGTCCAGGTCAGACAACGCCTTCCTCTCTGCCTGGGCTTCACGGATTGCCAGCCTCACA
  GGGTCAGTGGTGAACCTTGTCTTCATCCTCATCCTCTCCAAGGTCTATGTGCTCCTGGCCCAGGTCCTAA
  CAAGATGGGAGATGCACCGGACACAGACCGAGTTTGAGGATGCCTTCACCCTCAAGGTGTTCATCTTCCA
  GTTTGTGAATTTCTACGCCTCGCCCGTGTATATCGCCTTCTTCAAGGGCAGGTTTGTGGGATACCCGGGC
  AACTACCACACCTTGTTTGGAATCCGAAATGAGGAGTGTCCAGCTGGAGGCTGCCTCAGTGAGCTGGCAC
  AGGAGCTCCTGGTCATCATGGTGGGCAAGCAGATCATCAATAACGTGCAGGAGGTTCTTGTCCCAAAGCT
  GAAAGGCTGCTGGCAGAAGTTCTCCAGGGGCAAGAAGGCTGGCACGGGGACCCACCCAGCACCTTGGGAG
  GCCGACTATGAGTTGCTGCCTTGTGAGGGGCTGTTTCACGAATACCTTGAAATGGTGCTGCAGTTTGGAT
  TCGTCACCATCTTCGTAGCTGCCTGCCCGCTGGCGCCGCTTTTTGCCCTACTCAACAACTGGGTGGAGAT
  CCGCCTAGATGCGCGCAAGTTCGTGTGCGAGTATCGGCGCCCAGTGGCGGAGCGCGCCCAGGACATCGGC
  ATCTGGTTCCACATCCTAACGGGCCTCACACACCTCGCGGTCATCAGCAATGCCTTCCTGCTGGCCTTCT
  CCTCCGACTTCCTGCCACGTGTCTACTACAGCTGGACGCACGCCCCTGACCTGCATGGCTTCCTCAATTT
  CACGCTGGCACGAGCACCGCCCACTTTCACCTCCGCACACAACCGCACTTGCAGGTACCGGGCTTTCCGG
  GATGATGATGGACACTATTCTCCGACTTACTGGACTCTCCTGGCCATCCGCCTGGCCTTTGTCATCGTAT
  TTGAGCATGTGGTGTTCTCCATTGGCCGTGTTCTGGATCTCTTGGTTCCTGACATCCCAGAATCCGTGGA
  GATCAAGGTGAAGCGGGAATACTATTTAGCTAAACAAGCACTGGCTGAGAACGAGGTCACAGTAGGGGTT
  ACCGGAGGCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001271884
• Insert Size: 2532 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001271884.1, NP_001258813.1
• RefSeq Size: 3899 bp
• RefSeq ORF: 2532 bp
• Locus ID: 404545
• UniProt ID: Q14AT5
• Cytogenetics: 1 D
• Gene Summary: This gene encodes a member of the anoctamin family, which in mammals is comprised of 10 members. Anoctamin proteins are proposed to have eight transmembrane domains with both termini facing the cytoplasm and a C-terminal domain of unknown function. While some members have been characterized as calcium-activated chloride channels, this protein is reported to have little anion conductance activity. In humans, this protein is primarily found in prostate tissues and may serve as a target for prostate cancer immunotherapy. Alternative splicing results in multiple transcript variants that encode different isoforms. [provided by RefSeq, Dec 2012]
  Transcript Variant: This variant (2) uses an alternate acceptor splice site in the 3' coding region which results in a frameshift, compared to variant 1. It encodes isoform 2, which has a shorter and distinct C-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.