Tbc1d5 (NM_001285991) Mouse Untagged Clone

CAT#: MC229021

Tbc1d5 (untagged) - Mouse TBC1 domain family, member 5 (Tbc1d5), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tbc1d5
• Synonyms: 1600014N05Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229021 representing NM_001285991
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTATAAGTCTGTGTCTGAAACGAGACATCCTCTGCAGTCAGAAGAACAAGAAGTAGGCATTGACCCCT
  TGTTCAGTTATTCAAATAAAACAAGAGGAGATCTAAGTCAAAATGGAAGAGGATCAAATTCTACCTTAGA
  CACTGAAGGGACGTTCAATTCATATATGAAAGAATGGGAAGAACTATTTGTAAACAACAATTACTTGGCA
  ACAGTAAGACAGAAGGGGATTAATGGGCAGCTGAGAAGCAGCAGGTTCCGCAGCATTTGCTGGAAGCTCT
  TTCTTTGTGTTCTTCCTCAAGATAAAAGTCAGTGGATAAGTAAAATCAAAGAACTAAGAGCCTGGTATAG
  CAGTATTAAAGAAATACACATCACTAACCCAAGGAAGGCTGCCGGCCAGCAGGATCTGATGATCAACAAC
  CCCCTATCACAGGATGAAGGAAGTCTTTGGAACAAGTTCTTTCAAGATAAAGAACTACGGTCCATGATTG
  AGCAAGATGTCAAAAGAACGTTTCCTGAAATGCAGTTTTTCCAACAAGAAAATGTGAGAAAAATTCTTAC
  AGATGTTCTGTTCTGCTATGCAAGAGAGAACGAGCAGTTGCTTTACAAACAGGGTATGCATGAGCTACTA
  GCGCCTATCATCTTTACCCTCCACTGTGACCACCAAGCTTTTCTACATGCCAGCGAGTCTGCACAGCCCA
  GTGAGGAAATGAAAACTCTCCTGAACCCTGAGTATCTGGAGCATGATGCCTATGCAATGTTCTCACAGCT
  TATGGAAACTGCTGAACCTTGGTTTTCTACGTTTGAGCATGATGGTCAAAAGGGAAAAGAAACTCTGATG
  GCCCCCATTCCCTTTGCTCGACCACAGGATTTAGGGCCAACAGTTGCCATTGTTACAAAAGTCAACCAGA
  TCCAGGACCATCTACTGAAGAAACATGATATTGAGCTCTACATGCACCTGAACAGACTAGAAATTGCACC
  ACAAATATATGGGTTACGATGGGTGCGGCTGCTGTTTGGCAGAGAGTTCCCTCTGCAGGACCTGCTGGTG
  GTCTGGGATGCCCTGTTTGCGGATAGCCTCAACCTGAGCTTAGTCGACTATGTCTTCACAGCCATGCTAC
  TCTATATCCGAGATGCTTTGATTTCTAGTAACTATCAGACCTGTCTTGGCCTTCTGATGCATTATCCAAT
  CATCGGGGACATACACTCACTGATACTGAAGGCTCTGTTCCTTAGAGACCCAAAGAGAAATCCAAGGCCG
  GCGACTTACCAGTTCCACCCCAACTTAGACTACTACAAAGCACGGGGAGCCGACCTTATGAACAAAAGCC
  GGACCAATGCCAGAGGTGCTCCCCTGAATATACACAAGGTCTCCAACAGCTTGATTAACTTTGGAAGAAA
  GCTGATTTCCCCAGCCTCAGCTCCGGGCAGTATGGGTGGCCCTGTACCCGGAAACAACAGCAGCAGTTCC
  TTCTCTGCTGCCATCCCCACCAGGACCTCCACAGAAGCCCCAAGGCATCATTTGCTGCAGCAGCAACAGC
  AGCAGCAGCACCAGCAGCAGCAGCAGCAGCAACCACAGCAGCAGCAGCAGCAACACCAGCAGCAACAGCA
  GCAGCAGAGACTGATGAAATCTGAAAGCATGCCGGTACAGTTGAACAAAGGCGACGTAGTTACAGGAAGC
  GATGCTCAGGTTTCTGTTCCTGTCCAGGCTCTAACTGACCTCCAAGGGCAAAGTTCGAAAACCATCAGTT
  CATCTCCAAGTATCGAGAGTTTGCCTGGGGGAAGAGAATTCACTGGCTCTCCACCTCCATCCGCTACTAA
  AAAGGATTCCTTTTTCAGCAACATCGCACGTTCCCGTTCACACAGCAAAACCATGGGCAGGAAAGAGTCT
  GAAGAAGAACTAGAGGCTCAGATCTCCTTCCTACAAGGCCAGCTGAATGACCTGGATGCCATGTGCAAGT
  ACTGTGCAAAGGTCATGGACATGCATCTTGTAAACATTCAAGATGTGGTATTACAAGAGAATTTGGAAAA
  AGAAGATCAAATTCTGGTGTCCTTGGCAGGATTAAAACAGATCAAAGACATTCTGAAAGGTTCCCTGCGC
  TTCAACCAGAGCCAGCTGGAGGCTGGCGAGAATGAGCAGATTACCATCGCTGATGACCACTACTGCTCCA
  GTGGCCAGGACCAGGGCAGCCAAGTTCCTAGGGCCGCCAAGCAGGCCTCTTCAGAGATGCCAGGGTGCAC
  CGGTGGAACGACCCCAGATGACTTCATTCTGGTTTCCAAAGAAGATGAGGGGCACCGTGCCAGGGGGGCC
  TTCTCAGGCCAGGCCCAGCCTCTTCTCACCCTCAGAAGCACATCTGGGAAAAGCCGAGCCCCAGCCTGCT
  CCCCACTGCTGTTCTCAGACCCACTGATGGGCCCAGCCTCAGCCTCAGCCTCCTCCAGCAACCCCAGCTC
  CAGCCCCGATGATGACAGCAGCAAGGAGTCTGGCTTCACCATTGTGAGCCCCTTGGACATCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001285991
• Insert Size: 2514 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001285991.1, NP_001272920.1
• RefSeq Size: 5682 bp
• RefSeq ORF: 2514 bp
• Locus ID: 72238
• Cytogenetics: 17 C
• Gene Summary: May act as a GTPase-activating protein for Rab family protein(s). May act as a GAP for RAB7A. Can displace RAB7A and retromer CSC subcomplex from the endosomal membrane to the cytosol; at least retromer displacement seems to require its catalytic activity. Required for retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN); the function seems to require its catalytic activity. Involved in regulation of autophagy. May act as a molecular switch between endosomal and autophagosomal transport and is involved in reprogramming vesicle trafficking upon autophagy induction. Involved in the trafficking of ATG9A upon activation of autophagy. May regulate the recruitment of ATG9A-AP2-containing vesicles to autophagic membranes (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). Sequence Note: The RefSeq transcript was derived from the reference genome assembly. The genomic coordinates were determined from alignments.