Smarcad1 (NM_001253392) Mouse Untagged Clone

CAT#: MC229020

Smarcad1 (untagged) - Mouse SWI/SNF-related, matrix-associated actin-dependent regulator of chromatin, subfamily a, containing DEAD/H box 1 (Smarcad1), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Smarcad1
• Synonyms: AV081750; AW226546; D6Pas1; Etl1; mKIAA1122
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229020 representing NM_001253392
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGGAGCCATTGCTGCTGCCTTGCTGATGTTTGGTGATGCAGGTGGTGGACCCAGGAAAAGGAAAT
  TATCTTCTTCTTCTGAGGAGGATGACGTTAATGATGATCAGTCTGTAAAACAGCCGCGAGGGGACCGTGG
  AGAAGAATCAAATGAGTCTGCAGAAGCCAGCAGCAACTGGGAAAAGCAAGAAAGTATTGTGTTGAAATTG
  CAAAAGGAATTTCCAAATTTTGATAAACAGGAATTAAGAGAAGTGCTTAAGGAGCATGAGTGGATGTACA
  CAGAAGCCTTGGAGTCTCTGAAAGTGTTTGCGGAAGATCAAGACGTGCAGTGTGCTTCACAAAGTGAAGT
  TACAAATGGAAAAGAAGTTGCAAGGAATCAAAATTATTCTAAAAATGCAACTAAAATAAAAATGAAACAG
  AAAATTTCCGTGAAACCACAAAATGGCTTTAACAAGAAACGTAAGAAAAATGTATTTAATCCTAAGAAAG
  CCGTAGAGGACTCTGAGTACGATTCAGGTTCTGATGCTGGGAGCTCACTGGATGAGGACTATAGCAGTTG
  TGAAGAAGTGATGGAGGACGGCTATAAAGGCAAAATTCTTCATTTCCTTCAAGTTTCTTCAATTGCTGAA
  CTGACTTTGATTCCTAAGTGTTCTCAGAAAAAGGCTCAGAAAATAACAGAACTCCGGCCCTTTAATAATT
  GGGAAGCTCTGTTTACAAAGATGTCTAAAATCAATGGCCTATCTGAAGATTTGATATGGAATTGCAAAAC
  GGTAATTCAAGAAAGGGACGTAGTTATAAGACTTATGAACAAATGTGAGGACATTTCAAATAAACTGACC
  AAACAAGTTACGATGCTCACCGGGAATGGAGGCGGATGGAACAGAGAACAGCCCTCCCTTCTAAACCAAA
  GTTTGTCACTCAAGCCCTATCAGAAGGTTGGGTTGAACTGGCTGGCGTTGGTGCATAAGCATGGACTTAA
  TGGCATTCTGGCAGACGAAATGGGCCTAGGAAAAACCATTCAAGCCATTGCATTCCTGGCATACCTCTTT
  CAAGAGGGTAATAAAGGGCCTCATTTGATTGTTGTTCCAGCCTCCACCATAGATAACTGGTTAAGAGAAG
  TTAACTTATGGTGCCCCAGTTTAAATGTGCTCTGTTACTATGGTTCTCAAGAAGAGCGTAAGCAAATTAG
  ATTCAACATCCATAATAAGTATGAGGATTACAATGTAATTGTTACAACGTATAATTGTGCAATCAGCAGC
  TCTGATGACCGCAGTCTCTTTCGACGACTGAAACTGAATTATGCAATTTTTGATGAAGGCCATATGCTGA
  AGAATATGGGCTCTATCCGCTATCAACATCTAATGACAATTAATGCACGTAACCGTTTATTGCTCACAGG
  CACACCTGTGCAGAATAATCTGCTAGAACTCATGTCATTGTTGAATTTTGTTATGCCACACATGTTTAGC
  AGTAGCACCAGTGAAATCCGAAGGATGTTTTCTTCTAAAACGAAACCAGCAGACGAGCAGAGTATATACG
  AAAAGGAGAGAATAGCCCACGCGAAGCAAATCATTAAGCCCTTCATCCTCAGGAGAGTGAAGGAGGAGGT
  TCTTAAGCTCCTGCCTCCCAAGAAGGATCGGATTGAGCTATGTGCGATGTCAGAGAAGCAGGAGCAGCTC
  TACTCGGGCCTCTTCAACAGATTGAAAAAGTCTATCAACAACCTGGAAAAAAACACAGAGATGTGCAATG
  TCATGATGCAATTGAGAAAAATGGCCAATCACCCTTTACTACACCGCCAGTATTACACACCTGAGAAACT
  GAAGGAGATGTCTCAGCTAATGCTGAAGGAACCTACGCACTGTGAGGCCAACCCTGACCTGATCTTTGAA
  GACATGGAAGTTATGACAGATTTTGAACTACATGTACTTTGTAAACAGTATCAACACATTAATAGTTACC
  AGTTAGACATGGATTTAATTTTAGATTCTGGGAAATTCCGAGCCTTAGGATGCATCTTGTCTGAGTTGAA
  ACAGAAGGGTGATAGAGTTGTATTATTCAGCCAGTTTACCATGATGCTGGATATACTAGAGGTTCTCTTA
  AAGCATCATCAACATAGGTACCTCCGATTAGATGGAAAGACTCAGATTTCTGAAAGGATTCATCTAATTG
  ATGAGTTTAATACAGATATGGATATCTTTGTATTTCTCTTGTCAACTAAAGCTGGTGGACTAGGAATAAA
  TCTTACTTCAGCAAATGTTGTTATACTTCACGACATTGATTGCAATCCATACAATGACAAACAAGCAGAA
  GACAGGTGCCATAGAGTTGGTCAGACTAAAGAAGTATTAGTTATTAAATTAATAAGCCAAGGAACTATTG
  AAGAGTCCATGCTAAAAATTAACCAACAAAAGTTGAAACTAGAGCAAGACATGACCACAGTAGATGAAGC
  TGATGAAGGAAGTATGCCAGCAGATATAGCCACATTACTAAAAACATCAATGGGCCTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001253392
• Insert Size: 2511 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001253392.1, NP_001240321.1
• RefSeq Size: 4637 bp
• RefSeq ORF: 2511 bp
• Locus ID: 13990
• UniProt ID: Q04692
• Cytogenetics: 6 30.11 cM
• Gene Summary: DNA helicase that possesses intrinsic ATP-dependent nucleosome-remodeling activity and is both required for DNA repair and heterochromatin organization. Promotes DNA end resection of double-strand breaks (DSBs) following DNA damage: probably acts by weakening histone DNA interactions in nucleosomes flanking DSBs. Required for the restoration of heterochromatin organization after replication. Acts at replication sites to facilitate the maintenance of heterochromatin by directing H3 and H4 histones deacetylation, H3 'Lys-9' trimethylation (H3K9me3) and restoration of silencing (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an alternate exon which results in the use of a downstream start codon, compared to variant 1. The resulting protein (isoform 2) is shorter when it is compared to isoform 1.