Ampd2 (NM_001289720) Mouse Untagged Clone

CAT#: MC229019

Ampd2 (untagged) - Mouse adenosine monophosphate deaminase 2 (Ampd2), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ampd2
• Synonyms: 1200014F01Rik; AI552571; Ampd-2; m4521Dajl
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229019 representing NM_001289720
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCATCCTATCCTGGCCCAGGCAAGTCCAAGGCCAAATATCCCTTTAAGAAGCGGGCCGGCCTGCAAG
  CTTCCGCTGCCGCTCCAGAAGCTCGAAGCGGTCTGGGGGCCTCTCCGCTACAGTCTGCCCGATCCCTGCC
  AGGCAACGCCCCTTGCCTCAAGCACTTCCCGCTTGACCTGCGCACGTCTATGGATGGCAAATGCAAGGAG
  ATCGCTGAGGAGTTGTTCAGCCGCTCACTGGCTGAGAGTGAGCTTCGTAGCGCCCCTTATGAGTTCCCAG
  AGGAAAGCCCCATCGAGCAGCTAGAAGAACGGAGGCAGCGGCTGGAGCGCCAGATCAGCCAGGATGTCAA
  GCTGGAGCCAGATATTCTTCTTCGAGCCAAGCAAGATTTCCTGAAGACAGACAGCGACTCAGACTTACAG
  CTGTACAAGGAGCAAGGAGAGGGACAGGGTGACAGGGGTCTTTGGGAACGTGATGTGGTATTGGAACGGG
  AATTTCAGCGGGTCATCATCTCTGGGGAGGAGAAGTGTGGGGTGCCATTCACAGACCTCTTAGACGCAGC
  CAAAAGTGTGGTTCGGGCACTTTTCATCCGGGAGAAGTACATGGCCCTATCACTGCAGAGCTTCTGTCCC
  ACCACCCGCCGTTACCTGCAGCAGCTGGCTGAGAAGCCCCTGGAGACTCGAACTTATGAGCAGAGTCCTG
  ATACCCCTGTATCTGCTGATGCCCCAGTGCATCCCCCTGCACTGGAGCAGCACCCGTATGAGCACTGTGA
  GCCAAGCGCCATGCCTGGGGACCTGGGCTTGGGTCTGCGCATGGTGCGTGGTGTGGTGCACGTCTACACC
  CGCAGGGACCCTGATGAGCACTGTCCGGAGGTGGAGCTTCCATACCCTGACCTACAGGAATTTGTAGCTG
  ACGTCAATGTGCTGATGGCCCTGATCATCAATGGTCCCATAAAGTCATTCTGCTACCGCCGGCTGCAGTA
  CCTGAGCTCCAAATTCCAGATGCACGTTTTGCTCAATGAGATGAAGGAGCTCGCTGCTCAGAAGAAAGTG
  CCACACCGGGACTTCTACAATATCCGTAAGGTGGACACACACATCCACGCCTCGTCCTGCATGAACCAGA
  AACATCTACTGCGCTTCATCAAGCGGGCCATGAAGCGGCACCTGGAGGAGATTGTGCATGTGGAACAGGG
  CCGCGAGCAGACGCTGAGAGAAGTCTTCGAGAGCATGAACCTCACTGCCTACGACTTAAGTGTGGACACG
  CTGGATGTGCATGCGGACAGGAATACCTTTCATCGATTTGACAAATTCAATGCCAAATACAACCCTATTG
  GGGAGTCTGTTCTCCGAGAGATCTTCATTAAAACCGACAACAAGATTTCTGGGAAGTACTTTGCTCACAT
  CATCAAGGAGGTGATGGCAGACTTGGAGGAGAGCAAATACCAGAATGCAGAGCTCCGGCTGTCCATCTAC
  GGGCGTTCGAGGGATGAGTGGGACAAGCTGGCACGCTGGGCAGTGAACCACAAAGTGCACTCTCCCAATG
  TCCGCTGGCTGGTGCAGGTGCCCCGCCTCTTCGATGTGTACCGCACCAAGGGCCAGCTGGCCAACTTCCA
  AGAGATGCTGGAGAACATCTTTCTGCCCCTGTTTGAGGCTACTGTGCACCCTGCCAGCCACCCGGAGCTG
  CACCTCTTTCTGGAGCACGTGGATGGTTTTGATAGCGTGGATGATGAGTCCAAGCCAGAGAACCACGTCT
  TCAACCTGGAGAGTCCCCTCCCAGAAGCTTGGGTGGAGGAGGACAACCCTCCCTATGCCTACTACCTGTA
  CTACACCTTCGCTAACATGGCTATGTTGAACCATCTGCGCAGGCAGAGAGGTTTCCACACGTTCGTGCTG
  AGGCCGCACTGTGGGGAGGCCGGGCCCATCCACCACCTGGTATCAGCCTTCATGCTGGCCGAGAACATCT
  CCCACGGGCTGCTCCTGCGCAAGGCCCCCGTCCTGCAGTACCTGTATTACCTGGCTCAGATCGGCATCGC
  CATGTCCCCGCTCAGCAACAACAGCCTGTTCCTCAGCTACCACCGGAACCCTCTCCCTGAGTACTTGTCC
  CGTGGCCTCATGGTCTCGCTGTCCACAGATGATCCCTTGCAGTTCCACTTCACCAAGGTCAGACCCAGGC
  CAGCGGGCAGCCAAGGGCAGGAGCCCCTGATGGAGGAGTACAGCATCGCCACCCAGGTGTGGAAGCTCAG
  CTCCTGCGATATGTGCGAGCTGGCCCGTAACAGCGTGCTCATGAGTGGCTTCTCTCACAAGGTGAAAAGC
  CACTGGCTGGGACCCAACTATACCAAGGAGGGCCCTGAGGGCAATGATATCCGCCGTACCAACGTGCCAG
  ACATCCGAGTGGGCTACCGCTATGAGACGCTATGCCAGGAGCTGGCACTTATCACACAGGCCGTCCAAAG
  TGAGATGCTGGAGACCATCCCAGAGGAAGTGGGCATTGTCATGAGCCCAGGGCCTTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001289720
• Insert Size: 2508 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289720.1, NP_001276649.1
• RefSeq Size: 3458 bp
• RefSeq ORF: 2508 bp
• Locus ID: 109674
• UniProt ID: Q9DBT5
• Cytogenetics: 3 46.83 cM
• Gene Summary: AMP deaminase plays a critical role in energy metabolism. Catalyzes the deamination of AMP to IMP and plays an important role in the purine nucleotide cycle (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longer isoform (a).