Bcl11a (NM_001242934) Mouse Untagged Clone

CAT#: MC229018

Bcl11a (untagged) - Mouse B cell CLL/lymphoma 11A (zinc finger protein) (Bcl11a), transcript variant 4

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Bcl11a
• Synonyms: 2810047E18Rik; BCL-11A; Ctip1; D930021L15Rik; Evi9; Evi9a; Evi9b; Evi9c; mKIAA1809
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229018 representing NM_001242934
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTCGCCGCAAGCAAGGCAAACCCCAGCACTTAAGCAAACGGGAATTCTCGCCCGAACCTCTTGAAG
  CCATTCTTACAGATGATGAACCAGACCATGGCCCGTTGGGAGCTCCAGAAGGGGACCACGACCTTCTCAC
  CTGTGGGCAGTGCCAGATGAATTTCCCACTGGGGGACATTCTTATTTTTATCGAGCACAAACGGAAACAA
  TGCAATGGCAGCCTCTGCTTAGAAAAAGGTGTGGATAAGCCGCCTTCCCCTTCTCCCATCGAGATGAAAA
  AGGCATCCAATCCTGTGGAGGTTGGCATCCAGGTCACGCCAGAGGATGACGATTGTTTATCAACGTCATC
  TAGAGGAATTTGCCCCAAACAGGAACACATAGCAGATAAACTTCTGCACTGGAGGGGCCTGTCCTCTCCT
  CGGTCTGCACACGGAGCTCTAATCCCCACGCCCGGGATGAGTGCAGAATATGCCCCGCAGGGTATTTGTA
  AAGATGAGCCCAGCAGCTACACATGTACAACTTGCAAACAGCCATTCACCAGTGCATGGTTTCTCTTGCA
  ACACGCACAGAACACTCATGGATTAAGAATCTACTTAGAAAGTGAACACGGAAGTCCCCTGACCCCGCGG
  GTTGGTATCCCTTCAGGACTAGGTGCAGAATGTCCTTCCCAGCCACCTCTCCATGGGATTCATATTGCAG
  ACAATAACCCCTTTAACCTGCTAAGAATACCAGGATCAGTATCGAGAGAGGCTTCCGGCCTGGCAGAAGG
  GCGCTTTCCACCCACTCCCCCCCTGTTTAGTCCACCACCGAGACATCACTTGGACCCCCACCGCATAGAG
  CGCCTGGGGGCGGAAGAGATGGCCCTGGCCACCCATCACCCGAGTGCCTTTGACAGGGTGCTGCGGTTGA
  ATCCAATGGCTATGGAGCCTCCCGCCATGGATTTCTCTAGGAGACTTAGAGAGCTGGCAGGGAACACGTC
  TAGTCCACCGCTGTCCCCAGGCCGGCCCAGTCCTATGCAAAGGTTACTGCAACCATTCCAGCCAGGTAGC
  AAGCCACCCTTCCTGGCGACGCCCCCCCTCCCTCCTCTGCAATCCGCCCCTCCTCCCTCCCAACCCCCGG
  TCAAGTCCAAGTCATGCGAGTTCTGCGGCAAGACGTTCAAATTTCAGAGCAACTTGGTGGTTCACCGACG
  CAGCCATACTGGTGAGAAGCCCTATAAGTGCAACCTGTGCGACCACGCGTGCACACAGGCCAGCAAGCTG
  AAGCGTCACATGAAGACACACATGCACAAATCGTCCCCCATGACAGTCAAGTCCGACGATGGCCTCTCCA
  CAGCCAGCTCCCCGGAACCTGGTACCAGCGACCTGGTGGGCAGCGCCAGCAGTGCGCTCAAGTCAGTGGT
  GGCCAAGTTCAAGAGTGAGAACGACCCCAACTTGATCCCAGAGAACGGGGATGAGGAGGAAGAGGAGGAC
  GACGAGGAAGAAGAAGAAGAGGAGGAAGAGGAGGAGGAGGAGCTGACGGAGAGCGAGAGGGTGGACTACG
  GCTTCGGGCTGAGCCTGGAGGCTGCACGCCACCATGAGAACAGCTCTCGGGGCGCAGTGGTGGGCGTGGG
  CGACGAGGGCCGCGCCCTGCCCGATGTCATGCAGGGCATGGTGCTCAGCTCCATGCAGCACTTCAGCGAG
  GCCTTCCACCAGGTCCTGGGCGAAAAGCATAAGCGTAGCCACCTGGCCGAGGCCGAGGGCCATAGGGACA
  CTTGTGATGAAGACTCGGTGGCCGGTGAGTCAGACCGCATAGACGATGGCACTGTTAATGGTCGTGGCTG
  CTCCCCCGGCGAATCGGCTTCGGGGGGTCTGTCCAAAAAGCTGCTGCTGGGTAGCCCCAGCTCGCTGAGC
  CCCTTCTCCAAGCGCATCAAGCTGGAGAAGGAGTTTGACCTGCCCCCGGCCGCGATGCCTAACACGGAGA
  ACGTGTATTCGCAGTGGCTCGCTGGCTATGCGGCCTCCAGGCAGCTCAAAGATCCCTTCCTTACTTTCGG
  AGACTCCAGACAATCGCCTTTTGCCTCCTCATCAGAGCACTCCTCGGAGAACGGGAGCTTGCGCTTCTCC
  ACACCGCCCGGGGAGCTGGACGGAGGGATCTCAGGGCGCAGCGGCACAGGAAGTGGAGGGAGCACGCCCC
  ATATTAGTGGTCCGGGCCCGGGCAGGCCCAGCTCAAAAGAGGGCAGACGCAGCGACACTTGTGAGTACTG
  TGGGAAAGTCTTCAAGAACTGTAGCAATCTCACTGTCCACAGGAGAAGCCACACGGGCGAAAGGCCTTAT
  AAATGCGAGCTGTGCAACTATGCCTGTGCCCAGAGTAGCAAGCTCACCAGGCACATGAAAACGCATGGCC
  AGGTGGGGAAGGACGTTTACAAATGTGAAATTTGTAAGATGCCTTTTAGCGTGTACAGTACCCTGGAGAA
  ACACATGAAAAAATGGCACAGTGATCGAGTGTTGAATAATGATATAAAAACTGAATAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_001242934
• Insert Size: 2508 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001242934.1, NP_001229863.1
• RefSeq Size: 6122 bp
• RefSeq ORF: 2508 bp
• Locus ID: 14025
• UniProt ID: Q9QYE3
• Cytogenetics: 11 A3.2
• Gene Summary: Functions as a myeloid and B-cell proto-oncogene. May play important roles in leukemogenesis and hematopoiesis. An essential factor in lymphopoiesis, is required for B-cell formation in fetal liver. May function as a modulator of the transcriptional repression activity of ARP1.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (4) represents the longest transcript and encodes the longest protein (isoform 4).