Dlg1 (NM_001252436) Mouse Untagged Clone

CAT#: MC229016

Dlg1 (untagged) - Mouse discs, large homolog 1 (Drosophila) (Dlg1), transcript variant 5

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dlg1
• Synonyms: B130052P05Rik; Dlgh1; E-dlg/SAP97; mKIAA4187; SAP-97; SAP97
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229016 representing NM_001252436
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATTACATTTTCGGAAACAACACACTTCTGTACTCTCGCGCCAGTCGAGGAGGCAATGCTAGCTCTA
  GCCATGGCTCAGTAGGCCCTAAGCAGAAACACTGGGCAAAAAAGGGCTCGTCAGATGAACTGCAAGCCGA
  GCCAGAGCCTTCCCGCTGGCAGCAGATAGTTGCATTCTTCACTCGAAGACACAGCTTTATTGACTGCATC
  TCGGTAGCCACCAGCTCCACCCAGCCCACAGAAGCTGTTCCTCCCTCCTCTCCCATTGTCCCTGTGACCC
  CTGCCCTGCCAGTCCCTGCTGAGAGTACTGTCGTCCTGCCCTCCGCACCACAGGCAAATCCTCCTCCAGT
  GCTGGTCAACACAGACAGCTTAGAGACACCAACTTATGTTAATGGCACTGATGCAGATTATGAATATGAG
  GAAATCACACTTGAAAGGGGAAATTCGGGTCTTGGTTTCAGCATTGCAGGAGGTACAGACAACCCACACA
  TTGGAGATGACTCAAGTATTTTCATCACCAAAATTATCACAGGCGGAGCGGCTGCCCAGGATGGAAGATT
  GCGGGTAAATGACTGTATACTGAGAGTAAATGAAGCAGACGTTCGTGATGTAACCCACAGCAAAGCAGTG
  GAGGCATTAAAAGAAGCTGGATCTATTGTGCGATTGTATGTGAAAAGGCGGAAGCCAGCATCAGAAAAAA
  TCATGGAAATAAAGCTCATTAAAGGTCCTAAAGGTCTTGGGTTCAGCATTGCTGGAGGTGTTGGAAATCA
  GCACATTCCTGGTGATAACAGCATCTATGTAACCAAAATAATTGAAGGAGGTGCAGCACACAAGGATGGC
  AAGCTTCAGATTGGAGATAAGCTTCTAGCAGTGAACAGTGTGTGTTTAGAAGAAGTTACTCATGAAGAAG
  CAGTGACTGCCTTAAAGAATACATCTGATTTTGTTTATTTGAAAGTGGCAAAACCAACAAGTATGTATAT
  AAATGATGGCTATGCACCACCTGACATCACTAATTCTTCTTCTCAATCTGTTGACAACCATGTCAGCCCG
  TCCTCCTGCTTGGGCCAGACGCCAACGTCACCAGCCAGGTACTCACCCATTTCTAAAGCTGTGCTCGGAG
  ATGACGAGATCACTAGGGAACCTAGAAAAGTTGTTCTTCATCGTGGCTCAACAGGACTTGGTTTTAACAT
  TGTGGGAGGTGAAGATGGAGAAGGGATTTTTATCTCCTTCATCCTTGCTGGCGGACCTGCTGATCTAAGT
  GGAGAGCTCAGAAAAGGAGATCGCATCATATCGGTGAACAGTGTTGACCTCAGAGCTGCAAGTCACGAAC
  AAGCAGCAGCTGCACTAAAGAACGCAGGCCAAGCCGTCACCATCGTTGCGCAATATCGACCCGAAGAGTA
  CAGTCGTTTTGAAGCTAAAATCCATGACTTACGGGAGCAGATGATGAATAGCAGTGTCAGTTCAGGGTCA
  GGGTCTCTTCGAACCAGCCAGAAGCGCTCCCTCTATGTCAGAGCCCTCTTTGATTATGACAAGACTAAGG
  ACAGCGGGCTTCCCAGTCAAGGACTGAACTTCCGCTTTGGAGACATCCTCCATGTCATCAATGCTTCTGA
  CGACGAGTGGTGGCAAGCCAGGCAGGTCACCCCAGACGGGGAGAGTGACGAAGTCGGAGTGATTCCTAGT
  AAACGAAGAGTTGAGAAGAAGGAACGAGCCCGATTAAAAACGGTCAAATTCAATTCTAAAACAAGAGGAG
  ATAAAGGGGAGATCCCTGACGACATGGGATCAAAAGGCCTGAAGCACGTAACTTCTAATGCCAGCGATAG
  TGAAAGTAGTTACCTAATCTTGATTACAGATGAATATGGCTGCTCAAAAGGTGGTCAAGAAGAATATGTT
  TTATCTTATGAGCCAGTGAATCAACAAGAAGTTAATTATACCCGACCAGTCATCATATTAGGACCTATGA
  AAGACAGAGTAAATGATGACTTAATCTCAGAATTTCCTGACAAATTTGGATCCTGTGTCCCTCATACAAC
  TAGACCGAAGCGTGACTATGAGGTGGATGGACGAGATTATCATTTTGTGACTTCAAGGGAACAGATGGAA
  AAGGATATTCAGGAGCATAAGTTCATTGAAGCCGGCCAGTATAACAACCATCTGTATGGGACGAGCGTGC
  AGTCCGTGCGAGCAGTGGCAGAGAAGGGCAAACATTGTATCCTTGATGTGTCTGGAAATGCCATAAAGAG
  GTTGCAGATTGCACAGCTTTATCCAATATCTATTTTTATTAAACCCAAATCCATGGAAAATATCATGGAA
  ATGAACAAGCGCCTAACAGAAGAGCAGGCCAGAAAAACATTTGAGAGAGCCATGAAGCTGGAGCAGGAGT
  TCACTGAGCATTTCACAGCTATTGTCCAGGGAGACACGCTGGAGGACATTTACAACCAAGTGAAACAGAT
  CATCGAAGAACAGTCTGGGCCTTACATCTGGGTCCCAGCGAAAGAAAAGCTATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001252436
• Insert Size: 2505 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001252436.1, NP_001239365.1
• RefSeq Size: 4513 bp
• RefSeq ORF: 2505 bp
• Locus ID: 13383
• Cytogenetics: 16 22.4 cM
• Gene Summary: Essential multidomain scaffolding protein required for normal development. Recruits channels, receptors and signaling molecules to discrete plasma membrane domains in polarized cells. Regulates the excitability of cardiac myocytes by modulating the functional expression of Kv4 channels (By similarity). Functional regulator of Kv1.5 channel (By similarity). May play a role in adherens junction assembly, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (5) differs in the 5' UTR and has multiple differences in the coding region, including the use of an alternate start codon, compared to variant 1. The encoded isoform (5) is shorter and has a distinct N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.