Mcm8 (NM_001291054) Mouse Untagged Clone

CAT#: MC229015

Mcm8 (untagged) - Mouse minichromosome maintenance deficient 8 (S. cerevisiae) (Mcm8), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mcm8
• Synonyms: 5730432L01Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229015 representing NM_001291054
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGGTGCTTATAGAGGCAGAGGTTTTGGACGAGGAAGATTCCAAAGCTGGAAAAGAGGAAGAGGTG
  GTGGGAACTTCTCAGGAAGGTGGAGAGAAAGAGAAAACAGAGTTGACCTGAATGAAGCTTCAGGAAAGCA
  CGCTTCTGCACAAGCCTCACAACCGTTACTTCAACAGTCAACATTGGATCAGTTTATCCCGTATAAGGGC
  TGGAAGCTTTACTTCTCTGAAGTTTACAGTAATAACTCTCCCTTTATTGAGAAGATTCAAGCATTTGAGA
  AATTTTTCACAAGGCATATCGACTTATACGATAAGGATGAAATAGAAAGAAAAGGAAGCATTTTGGTGGA
  TTTTAAAGAACTGACAAAAGCTGATGAAATAACTAACTTGATACCCGATATAGAAAACGCACTAAGAGAT
  GCCCCTGAGAAAACACTGGCGTGTATGGGGCTGGCAATACATCAGGTATTAACTAAGGACCTTGAAAGGC
  ATGCCGCCGAATTACAAGCTCAAGAGGGATTGTCTAACGGTGGAGAGACAATGGTAAATGTACCACATAT
  TTATGCAAGAGTGTACAACTATGAGCCCTTGACACACCTCAAGAATATCCGAGCAACTTGCTATGGGAAA
  TACATCTCTATACGAGGGACTGTGGTCCGGGTCAGCAACATAAAGCCTCTTTGCACCAACATGGCTTTTC
  AGTGCGCTGCATGTGGAGAGATTCAGAGCTTTCCTCTGCCAGATGGAAAATACACCCTTCCTACAAAGTG
  TCCTGTGCCTGCGTGCCGAGGGAGGTCATTTGCTCCACTGCGCAGCTCTCCTCTCACAGTTACACTGGAC
  TGGCAGTTGATCAAAATCCAGGAGCTGATGTCTGATGCACAGAGAGAAGCTGGTCGGATCCCTCGGACGA
  TAGAATGTGAACTTGTTCACGATCTTGTAGATAGTTGTGTCCCAGGAGATACAGTGACTGTTACTGGAAT
  TGTCAAAGTCTCCAATTCTGAAGAAGGTTCTCGAAATAAGAATGATAAGTGCATGTTCCTTTTGTACATT
  GAGGCAAATTCTGTTAGCAACAGCAAGGGGCCGAAAGCACAGACTGCAGAAGATGGTTGCAAGCATGGGA
  CACTGATGGAGTTCTCCCTTAAAGACCTCTATGCCATCCGAGAGATCCAGGCTGAAGAGAACCTGCTCAA
  GCTCGTTGTCAACTCACTCTGTCCTGTCATTTTTGGTCATGAACTCGTCAAAGCAGGTCTGACGTTAGCA
  CTCTTTGGCGGTAGCCAGAAGTACGCAGATGACAAAAACAGAATTCCCATTCGAGGAGACCCACATGTCC
  TGATTGTTGGAGATCCAGGCTTGGGGAAGAGTCAGATGCTACAGGCAGCATGCAACGTGGCGCCACGTGG
  TGTGTATGTTTGTGGAAATACCACCACCAGCTCTGGTCTCACTGTGACTCTTTCAAAGGACAGTTCCTCT
  GGAGATTTTGCTTTGGAAGCTGGTGCCCTTGTACTTGGTGACCAAGGCATCTGTGGAATAGATGAATTTG
  ATAAAATGGGGAACCAACATCAAGCCCTGTTAGAAGCCATGGAACAGCAGAGTATTAGCCTTGCGAAGGC
  TGGGGTAGTTTGCAGCCTCCCTGCAAGAACTTCCATTATTGCTGCTGCAAATCCAGTCGGAGGACACTAC
  AATAAAGCCAGAACGGTTTCTGAGAATTTAAAGATGGGGAGTGCCCTACTGTCCAGATTCGATTTGGTCT
  TTATCTTGTTAGATACGCCAAATGAACAGCATGACCACTTACTTTCTGAACATGTGATCGCGATAAGAGC
  TGGGAAGCAGAAAGCGGTTAGCAGTGCCACAGTCACTCGTGTGCTCAGTCAAGACTCAAATACTTCTGTA
  CTTGAAGTGGTTTCTGAGAAACCATTATCAGAAAGACTAAAGGTGGCTCCTGGAGAACAGACAGATCCGA
  TTCCACATCAGCTGTTGAGGAAGTACATTGGCTATGCACGGCAGTATGTCCACCCAAGGTTATCTACGGA
  CGCTGCTCAGGCCCTTCAGGATTTCTACCTGGAGCTCCGCAAGCAGAGCCAGCGCGTGGGCAGCTCACCC
  ATCACCACTCGGCAGCTGGAGTCTTTGATCCGTCTGACAGAGGCACGGGCAAGGTTAGAATTGAGAGAGG
  AAGCAACTAGAGAAGATGCTGAAGATATAATTGAAATTATGAAGCATAGCATGCTAGGAACTTACTCAGA
  TGAATTCGGAAACCTGGACTTTGAGCGATCCCAGCATGGCTCTGGGATGAGCAACAGGTCAACAGCAAAA
  AGATTTATTTCTGCTCTCAACAGCATTGCTGAAAGAACTTATAACAACATATTTCAATATCATCAACTTC
  GTCAGATTGCTAAAGAACTAAACATTCAGGTTGCCGATTTTGAAAACTTCATTGGATCACTGAATGACCA
  AGGTTATCTCTTGAAGAAAGGCCCAAAGATTTACCAACTTCAAACTATGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001291054
• Insert Size: 2502 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001291054.1, NP_001277983.1
• RefSeq Size: 3481 bp
• RefSeq ORF: 2502 bp
• Locus ID: 66634
• UniProt ID: Q9CWV1
• Cytogenetics: 2 F2
• Gene Summary: Component of the MCM8-MCM9 complex, a complex involved in the repair of double-stranded DNA breaks (DBSs) and DNA interstrand cross-links (ICLs) by homologous recombination (HR). Required for DNA resection by the MRE11-RAD50-NBN/NBS1 (MRN) complex by recruiting the MRN complex to the repair site and by promoting the complex nuclease activity. Probably by regulating the localization of the MNR complex, indirectly regulates the recruitment of downstream effector RAD51 to DNA damage sites including DBSs and ICLs. The MCM8-MCM9 complex is dispensable for DNA replication and S phase progression. However, may play a non-essential for DNA replication: may be involved in the activation of the prereplicative complex (pre-RC) during G(1) phase by recruiting CDC6 to the origin recognition complex (ORC) (By similarity). Probably by regulating HR, plays a key role during gametogenesis (PubMed:22771120). Stabilizes MCM9 protein (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a).