Adam8 (NM_001291066) Mouse Untagged Clone

CAT#: MC229011

Adam8 (untagged) - Mouse a disintegrin and metallopeptidase domain 8 (Adam8), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Adam8
• Synonyms: ADAM 8; CD156; CD156a; E430039A18Rik; MS; MS2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229011 representing NM_001291066
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTTGGCCTCTGGCTGCTCAGCGTCTTATGGACACCAGTAGCCCCTGGACCTCCTTTGCCCCATGTGA
  AACAGTATGAAGTGGTTTGGCCTCGGCGCCTAGCTGCATCCCGCTCCCGCAGAGCCCTGCCCTCCCACTG
  GGGCCAGTACCCAGAGAGTCTGAGCTATGCTCTTGGGACCAGCGGGCACGTTTTCACCCTGCACCTTCGA
  AAGAACAGGGACCTGCTGGGCTCAAGCTACACAGAGACCTACTCAGCTGCCAATGGCTCTGAGGTGACAG
  AGCAACTGCAGGAGCAGGACCATTGCCTCTACCAAGGCCATGTGGAAGGGTACGAGGGCTCAGCTGCCAG
  TATTAGCACCTGTGCTGGCCTCAGGGGCTTTTTCCGAGTTGGGTCCACTGTCCACTTGATTGAGCCTCTG
  GATGCTGATGAAGAGGGGCAACATGCGATGTATCAGGCAAAGCATCTGCAACAGAAGGCTGGGACCTGTG
  GGGTCAAAGATACCAACCTGAATGACCTAGGGCCTCGGGCATTAGAAATATACAGGGCTCAGCCACGGAA
  CTGGCTGATACCCAGAGAAACCCGCTATGTGGAGTTGTATGTGGTTGCAGACAGCCAAGAGTTCCAGAAG
  TTGGGGAGCAGAGAGGCCGTGCGCCAGCGAGTGCTGGAGGTTGTAAACCACGTGGACAAGCTTTATCAGG
  AACTCAGTTTTCGAGTTGTCCTGGTGGGCCTGGAGATCTGGAACAAGGACAAATTCTACATCAGCCGCTA
  TGCCAACGTGACACTGGAGAACTTCTTGTCCTGGAGGGAACAGAACTTGCAAGGGCAGCACCCACATGAC
  AACGTGCAACTTATCACGGGGGTTGATTTCATTGGGAGCACTGTTGGACTGGCTAAGGTGTCTGCCCTGT
  GTTCCCGTCACTCCGGAGCTGTGAATCAGGACCACTCCAAGAACTCCATTGGTGTAGCCTCCACCATGGC
  CCATGAGCTGGGCCACAACCTGGGCATGAGCCATGATGAGGACATTCCAGGATGCTACTGTCCTGAACCA
  CGGGAGGGTGGTGGCTGCATCATGACCGAAAGCATCGGCTCCAAGTTCCCCAGGATATTCAGCAGGTGTA
  GCAAGATTGACCTAGAGTCATTCGTGACAAAACCCCAGACAGGCTGCCTGACCAATGTTCCAGATGTCAA
  CCGGTTCGTGGGTGGCCCTGTGTGTGGAAACCTGTTTGTGGAGCATGGAGAGCAGTGTGACTGTGGCACA
  CCTCAGGACTGTCAAAACCCCTGCTGCAATGCCACCACTTGCCAGCTGGTCAAGGGGGCAGAGTGTGCCA
  GTGGTACCTGTTGTCATGAATGCAAGGTGAAGCCAGCTGGAGAGGTGTGTCGTCTCAGTAAGGACAAATG
  TGACCTGGAGGAGTTCTGTGATGGCCGGAAGCCAACATGTCCCGAAGATGCCTTCCAACAGAATGGCACT
  CCCTGCCCAGGGGGCTACTGCTTTGATGGGAGCTGTCCCACCCTGGCACAGCAGTGCCGGGATCTGTGGG
  GGCCAGGTGCTCGGGTAGCAGCCGACTCCTGCTATACCTTTAGCATCCCTCCGGGCTGCAATGGGAGGAT
  GTACTCTGGCAGGATCAACCGGTGTGGAGCGCTGTACTGTGAGGGAGGCCAGAAGCCCCTTGAACGCTCC
  TTCTGCACTTTCTCCTCCAACCATGGAGTCTGCCATGCTCTTGGCACAGGCAGCAACATTGACACCTTTG
  AGCTGGTATTGCAGGGCACCAAGTGCGAGGAGGGAAAGGTTTGCATGGATGGAAGCTGCCAGGACCTCCG
  TGTATACAGATCTGAAAACTGCTCTGCTAAATGCAACAACCATGGGGTATGCAACCACAAGAGGGAGTGC
  CACTGTCACAAGGGCTGGGCACCACCCAACTGTGTACAGCGGCTGGCAGATGTATCAGATGAACAAGCAG
  CGTCTACGAGCCTCCCAGTCAGTGTGGTTGTGGTCTTGGTGATCCTGGTGGCTGCGATGGTCATCGTGGC
  AGGCATCGTCATCTACCGAAAGGCTCCGAGACAAATCCAGAGGAGGAGTGTGGCACCCAAGCCTATCTCG
  GGGCTCTCCAACCCCCTATTCTACACAAGGGACAGCAGCCTGCCAGCTAAGAACAGGCCTCCAGACCCTT
  CTGAGACAGTTTCTACCAACCAGCCCCCAAGACCCATAGTGAAACCAAAGAGGCCTCCCCCTGCACCTCC
  AGGTGCTGTGTCCAGTTCACCACTCCCAGTTCCTGTTTATGCCCCAAAGATACCAAATCAGTTTAGACCT
  GATCCTCCCACCAAGCCCCTCCCAGAGCTGAAACCCAAGCAGGTCAAGCCAACCTTTGCACCCCCGACAC
  CACCAGTCAAGCCCGGGACTGGAGGGACGGTGCCTGGAGCAACTCAGGGAGCTGGTGAGCCAAAGGTTGC
  TCTGAAGGTCCCCATCCAGAAGAGGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001291066
• Insert Size: 2478 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001291066.2, NP_001277995.1
• RefSeq Size: 3114 bp
• RefSeq ORF: 2478 bp
• Locus ID: 11501
• Cytogenetics: 7 F4
• Gene Summary: This gene encodes a member of the Adam family of proteins that contain the disintegrin and metalloprotease domains. The encoded protein is localized to the cell surface, where it is involved in the remodeling of extracellular matrix and cell migration. Mice lacking the encoded protein display persistent inflammation upon treatment with allergens. Alternative splicing of this gene results in multiple variants. [provided by RefSeq, Mar 2015]
  Transcript Variant: This variant (2) uses an alternate in-frame splice acceptor site compared to variant 1. It encodes a protein (isoform 2) that is slightly shorter than isoform 1.