2010111I01Rik (NM_001289924) Mouse Untagged Clone

CAT#: MC229007

2010111I01Rik (untagged) - Mouse RIKEN cDNA 2010111I01 gene (2010111I01Rik), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: 2010111I01Rik
• Synonyms: 2300006M17Rik; Aopep; AP-O; ApO; AW742919; mir-23b; mir-24-1; mir-27b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229007 representing NM_001289924
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACATAAAGCTGGACCCTTCCCGGGATGATCTGCCTCTCATGGCCAACACCAGCCACATGCTTGTGA
  AGCACTACATACTGGATTTGGATGTGGACTTTGGAAATCAAGTCATTGAGGGCAACATAGTGCTTTTCTT
  TGGAGATGGAAACAGATTTAAAAACCAGTCGCGTTCTACCCAGGAAACCTTCCAGATGGAGTCAGAGGAG
  GCTGACATATTTAGGACAGCTGAACCCTGCCATGTTCCTGAGATGGATTCGAGTACCTTCTCACCTAAGA
  TGGGACATAGAGAATGTGCAGTCTGTGGTAAAGGTGATCAAGATGCCTTTGATAACGATGGTAACCATGA
  CAACCAGGAACGTGATTCTGAGATCTCTAGCTCAAAGTACTGCTGTGACACAGGGAATCATGGGAAAAGG
  GATTTCTTGCTAGTGTTGGACTGCTGTGATTTATCTGTGCTAAAGGTAGAGGAGGTGGATGTGGCTGCTG
  TGCCAGGCCTTGAGAAATTTACTAAAGCTCCAAAGCTCTTGGCTACTCCTGAGAAGCTCAGGTGTGAGAT
  TGTCCGTGACCTTGTGGCTCTGCCTGCAGATGCTTGGAGGGAGCAGTTAGACTGCTACACTCGTTGCAGC
  CAGGCTCCTGGCTGTGGGGAGCTCTTGATTGACTCTGACAATTGGAGCTTACGGATCAGGAAGACGGGGA
  CTTCGACACCTGCTGACTTTCCTCGTGCCATCAGAATATGGTATAAAACTAAACCTGAGGGGCAGTCAGT
  AGCTTGGACAACAGACCAGAATGGCAGGCCATGTGTTTATACTATGGGATCCCCCATCAACAACAGGGCC
  CTTTTTCCATGCCAGGAACCACCCGTTGCCATGTCAACATGGCAGGCTACAGTTCGAGCAGCTGCATCTT
  TTGTTGTTTTAATGAGTGGAGAAAATTCTGCCAAGCCCACACCACTTCGAGAAGGATACATGAGCTGGCA
  TTACTATGTGACCATGCCAATGCCGGCCTCTACCTTTGCAATTGCAGTGGGGTGCTGGACAGAAATGAAG
  CCCAAGGCATCCCCACCAGATGATCTGATGACTGAGCACTCTCTCCCCCTCTCGCCATCGGAAGCGGACT
  TGAGGTATGATAACACCTGCAATCATATGGAATACCCTTGCAGGTTCCAGAGTGCGTCCGCTGCCTCCCA
  GGACATCATTCCTTATCGAGTGTTTGCCCCAGTGTGCCTTGAGGGTGCCTGCCAAGAGGCCCTGCTGTGG
  CTGATCCCTTCTTGCCTCTCAGCTGCACACTCTGTCCTGGGAACACACCCTTTCTCCCGGCTGGACATAC
  TCATTGTCCCCACCAACTTTCCAAGTCTGGGAATGGCCAGCCCACACATCATCTTCCTCTCTCAGAGCAC
  CTTAACAGGCACGAGCCATCTCTGTGGGACCCGTCTTTGCCATGAAATTGCTCACTCCTGGTTTGGCCTA
  GCCATTGGGGCCCGAGACTGGACAGAGGAGTGGCTCAGTGAAGGATTTGCCACGCATTTGGAAGATATAT
  TTTGGGCTGAGGCACAGCAGCTGCCCCCCCATGAGGCCCTGGAGCAGCAGGAGCTGAGGGCTTGCCTGCG
  CTGGCACCGCCTGCAGGATGAGCTTCGGAACTCCCCAGAGGGAATGCAGGTGTTAAGACCCAACAAAGAA
  GAGACTGGCCATGTGAGTGCTTCAGGTGCATCTGTTGTCAAGCATGGACTCAATCCAGAGAAGGGCTTCA
  TGCAGGTTCATTACTTAAAGGGCTACTTCCTTCTTCGGTTCCTAACCAGAACACTTGGAGAGAAAATTTA
  TTTTCCGTTTTTAAGAAAATTTGTGCATCTGTTTCATGGGCAGCTGATTCTTTCCCAGGATTTTCTTCAA
  ATGCTGTTGGAGAACATTCCAGAAAACAAAAGGCTCGGCCTGTCTGTTGAGAACATCGTCCGAGATTGGC
  TTGAGTGTTCCGGAATACCTAAGGCGCTGCAGGAGGAGCGCAAGGCCGAGGACTGCTCGCCGAGTAGGCT
  CGCACGGCAAGTAGGCTCCGAGGTGGCGAAATGGATTCGAGTCAACCGCAGACCCCGAAAACGCAAACGA
  GGGAAGCGAGAAGTCGCCTTTGAAAAGCTTTCTCCAGACCAGATCGTCTTGCTTTTGGAGTGGCTCTTAG
  AGCAGAAGACGCTGAGCCCTCAGACACTGCACTGTCTCCAGCAGACTTACCATCTCCCAGAGCAGGATGC
  AGAGGTTCGCCATCGATGGTGTGAACTGGTTATTAAGCACAAGTACACAAAGGCATACAATCAGGTGGAG
  AGGTTCCTACTGGAGGACCAGGCCATGGGCATATACCTGTATGGGGAGCTGATGGTGAGTGAGGACGCCA
  GGCTGCAGCAGCTAGCCCACAGGTGCTTTGAGCTGGTGAAGGAACACATGGACAGAGCATCGGCCCAGGT
  GGTGACTGAAATGCTGTTCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001289924
• Insert Size: 2472 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289924.1, NP_001276853.1
• RefSeq Size: 3843 bp
• RefSeq ORF: 2472 bp
• Locus ID: 72061
• UniProt ID: Q8BXQ6
• Cytogenetics: 13 B3
• Gene Summary: Aminopeptidases catalyze the hydrolysis of amino acid residues from the N-terminus of peptide or protein substrates. Able to cleave angiotensin III to generate angiotensin IV, a bioactive peptide of the renin-angiotensin pathway. Not able to cleave angiotensin I and angiotensin II. May play a role in the proteolytic processing of bioactive peptides in tissues such as testis and heart.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the functional protein. Variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.