Nfx1 (NM_001290448) Mouse Untagged Clone

CAT#: MC228992

Nfx1 (untagged) - Mouse nuclear transcription factor, X-box binding 1 (Nfx1), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nfx1
• Synonyms: 1300017N15Rik; 3000003M19Rik; NFX.1; TEG-42; Tex42
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228992 representing NM_001290448
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGGCGCCTCCGGTCTCAGGCACTTTTAAATTCAATACAGATGCTGCTGAGTTCATTCCTCAGG
  AGAGAAAAACTTCTGGTCTAAATTGTGGGACCCAAAGAAGACTAGATTCTAGTAGGATTGGCAGAAGAAA
  TTATAGTTCCTCGCCTCCCTGTCACCTTCCTAGACACATCCCTTATGAAGACATCTCTGCTGTTCATCAG
  CACAGTTATGCCTCTGGAAGCAAACCTAAGAGTCCCCAGGGTTTTTTCCAGTCATCTAATAAATCACTCA
  AGAACCATGGCCTTCAGAATCAGCCATGGCAGAAAGCAAGGAATGAAAAGCACCAAAACAGAAACAAGAA
  AGCACAAGGGCTCTCTGAGCAGACGTCAGATACATCTAGTTTAGAAAGTGTAGCCAGGTCAGAGAGTGGA
  ACAAACCCGAGAGAGCACAGCCCTTCCGAGAGTGAGAAGGAGGTGGTTATCGCAGATCCCAGGGGAGCGA
  AACCCAAAAAAGCAGCACAGCTTACATACAACTATGGCAGAGGACCAAAGGCCAAAGGGAGACTCAGAAG
  CGAGTGGGGTAATAGAATGAGCCCAAAGTCTGAGGACGAAAATACCAGACCCGTGGCGATTTCCCACACT
  GACTCTTCAGATGCCTCCTGTAGAAAACCTGTAGTGGATCCATGTGTGTGCAGGCGGAATGAGCAGAGAA
  GATATCCCCAGAAAAGGCCTCCCTGGGAAGTGGAAGGGGCCAGACCACGGCCAGGCAGGAACCCACCAAA
  ACAGGAGAGTCAGCGGCATATAAATGCAGGGCCCAAAACCAACATGTCCCCCATTCCAAAGGATAATCTT
  AGAGAAAGACCAACGAAGTCAGCCTGTGACACTGGGAATTTGGCAGTTGTCAGCAAGTCTTCGAGAAGGG
  TTAACCAAGAGAAAACTGCTGTAAGGAGACAGGATCCCCAAGTGCTATCTCCTTTTCCCAGAGGCAAACA
  GAACCATATGCTGAAGAATGTGGAAACACATACAGGTTCCCTGATTGAGCAGCTAACTACAGAAAAATAC
  GAGTGTATGGTATGCTGTGAGTTGGTTCAGGTCACAGCCCCGGTGTGGAGCTGTCAGAGCTGTTTTCATG
  TCTTTCATCTGAACTGCATCAAGAAGTGGGCACGCTCTCCAGCATCGCATGCAGATGGCCAGAGTGGTTG
  GAGGTGCCCTGCTTGTCAGAATGTTTCTGCACATGTTCCCAATACCTATACTTGTTTCTGTGGCAAAGTA
  AAGAATCCTGAATGGAGCAGAAATGAAATTCCACATAGTTGTGGTGAGGTTTGTAGAAAGAAACAGCCTG
  GCCAGGACTGTCCACATTCCTGTAACCTTCTCTGCCATCCTGGACCCTGCCCACCCTGCCCTGCTTTTAC
  AACTAAAACATGTGAATGTGGACGGACCAGGCACACGGTTCGCTGTGGTCAGCCTGTCTCAGTCCACTGT
  TCTAATGCATGTGAGAATATCTTGAACTGTGGTCAGCACCACTGTGCTGAGCTGTGCCATGGGGGTCAAT
  GCCAGCCTTGTCGGATCATACTGAACCAGGTGTGCTACTGTGGCAGCACCTCCCGAGATGTGTTGTGTGG
  AACCGATGTGGGAAAGTCTGATGGATTTGGGGACTTCAGCTGTTTAAAGATATGTGGCAAGGACTTGAAG
  TGTGGGAGCCATACCTGTTCTCAGGTTTGCCACCCTCAGCCGTGTCAGCCTTGCCCACGGCTCCCCCATT
  TGGTGCGATACTGCCCTTGCGGCCAAACACCTCTCAGCCAGTTGCTGGAACATGGAAGTAATGCTCGGAA
  AACATGCATGGATCCTGTCCCTTCCTGTGGAAAAGTGTGTGGCAAACCCTTGGCCTGTGGTTCCTCAGAT
  TTCATCCACACCTGTGAAAAGCTTTGCCATGAAGGAGACTGTGGGCCGTGCTCTCGCACATCAGTCATTT
  CCTGCAGATGCTCCTTCAGAACAAAGGAACTTCCGTGTACCAGTCTTAAAAGTGAAGATGCTACATTTAT
  GTGCGACAAACGGTGTAACAAGAAACGATTGTGCGGACGGCATAAATGTAACGAGATCTGCTGTGTGGAC
  AAGGAGCACAAGTGTCCTTTGATTTGTGGGAGGAAACTCCGCTGTGGCCTTCATCGGTGTGAAGAACCTT
  GTCATCGAGGGAACTGTCAGACCTGCTGGCAAGCCAGCTTTGATGAATTAACCTGCCACTGTGGCGCCTC
  TGTGATCTACCCACCAGTTCCCTGTGGGACTAGGCCTCCTGAGTGTACCCAAACCTGTGCCAGGATCCAT
  GAATGTGACCATCCAGTATATCATTCTTGTCATAGCGAGGAGAAGTGTCCCCCTTGTACTTTCCTAACTC
  AGAAGTGGTGCATGGGCAAGCATGAGGAGCTCACAATTAAGAAACTTTGGACTTTTAAAGAGACTTTGGA
  CTTTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001290448
• Insert Size: 2457 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001290448.1, NP_001277377.1
• RefSeq Size: 4368 bp
• RefSeq ORF: 2457 bp
• Locus ID: 74164
• UniProt ID: B1AY10
• Cytogenetics: 4 20.72 cM
• Gene Summary: Binds to the X-box motif of MHC class II genes and represses their expression. May play an important role in regulating the duration of an inflammatory response by limiting the period in which MHC class II molecules are induced by interferon-gamma. Together with PABPC1 or PABPC4, acts as a coactivator for TERT expression. Mediates E2-dependent ubiquitination.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks several exons in the 3' region and uses an alternate 3' exon structure, compared to variant 1. It encodes isoform 2, which is shorter and has a distinct C-terminus, compared to isoform 1.