Ldlr (NM_001252658) Mouse Untagged Clone

CAT#: MC228983

Ldlr (untagged) - Mouse low density lipoprotein receptor (Ldlr), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ldlr
• Synonyms: Hlb301
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228983 representing NM_001252658
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCACCGCGGATCTGATGCGTCGCTGGGTCATCGCCCTGCTCCTGGCTGCTGCCGGAGTTGCAGCAG
  AAGACTCATGCAGCAGGAACGAGTTCCAGTGTAGAGACGGAAAATGCATCGCTAGCAAGTGGGTGTGCGA
  TGGCAGCCCCGAGTGCCCGGATGGCTCCGATGAGTCCCCAGAGACATGCATGTCTGTCACCTGTCAGTCC
  AATCAATTCAGCTGTGGAGGCCGTGTCAGCCGATGCATTCCTGACTCCTGGAGATGTGATGGACAGGTAG
  ACTGTGAAAATGACTCAGACGAACAAGGCTGTCCCCCCAAGACGTGCTCCCAGGATGACTTCCGATGCCA
  GGATGGCAAGTGCATCTCCCCGCAGTTTGTGTGTGATGGAGACCGAGATTGCCTAGATGGCTCTGATGAG
  GCCCACTGCCAGGCCACCACTTGTGGCCCCGCCCACTTCCGCTGCAACTCATCCATATGCATCCCCAGTC
  TTTGGGCCTGCGACGGGGATGTCGACTGTGTTGACGGCTCCGATGAGTGGCCACAGAACTGCCAGGGCCG
  AGACACGGCCTCCAAAGGCGTTAGCAGCCCCTGCTCCTCCCTGGAGTTCCACTGTGGTAGCAGTGAGTGT
  ATCCATCGCAGCTGGGTCTGTGACGGCGAGGCAGACTGCAAGGACAAGTCAGATGAGGAGCACTGCGCGG
  TGGCCACCTGCCGACCTGATGAATTCCAGTGTGCAGATGGCTCCTGCATTCACGGTAGCCGCCAGTGTGA
  CCGTGAACATGACTGCAAGGACATGAGCGACGAGCTCGGCTGCGTCAATGTGACACAGTGTGATGGCCCC
  AACAAGTTCAAGTGTCACAGTGGGGAGTGCATCAGCTTGGACAAGGTGTGCGACTCCGCCCGCGACTGCC
  AGGACTGGTCGGATGAGCCCATCAAGGAGTGCAAGACCAACGAGTGTTTGGACAACAATGGTGGCTGTTC
  CCACATCTGCAAGGACCTCAAGATTGGCTCTGAGTGCCTGTGTCCCAGCGGCTTCCGGTTGGTGGACCTC
  CACAGGTGTGAAGATATTGACGAGTGTCAGGAGCCAGACACCTGCAGCCAGCTCTGTGTGAACCTGGAAG
  GCAGCTACAAGTGTGAGTGCCAGGCCGGCTTCCACATGGACCCACACACCAGGGTCTGCAAGGCTGTGGG
  CTCCATAGGCTATCTGCTCTTCACCAACCGCCACGAGGTCCGGAAGATGACCCTGGACCGCAGCGAGTAC
  ACCAGTCTGCTCCCCAACCTGAAGAATGTGGTGGCTCTCGACACGGAGGTGACCAACAATAGAATCTACT
  GGTCCGACCTGTCCCAAAAAAAGATCTACAGCGCCCTGATGGACCAGGCCCCTAACTTGTCCTACGACAC
  CATCATCAGTGAGGACCTGCATGCCCCTGACGGGCTGGCGGTAGACTGGATCCACCGCAACATCTACTGG
  ACAGATTCAGTCCCAGGCAGCGTATCTGTGGCTGACACCAAGGGCGTAAAGAGGAGGACACTGTTCCAAG
  AGGCAGGGTCCAGACCCAGAGCCATCGTAGTGGACCCTGTGCATGGCTTCATGTACTGGACAGATTGGGG
  AACACCCGCCAAGATCAAGAAAGGGGGTTTGAATGGTGTGGACATCCACTCACTGGTGACCGAAAACATC
  CAGTGGCCAAATGGCATCACACTAGATCTTTCCAGTGGCCGTCTCTATTGGGTTGATTCCAAACTCCACT
  CTATCTCCAGCATCGATGTCAATGGGGGCAATCGGAAAACCATTTTGGAGGATGAGAACCGGCTGGCCCA
  CCCCTTCTCCTTGGCCATCTATGAGGACAAAGTGTATTGGACAGATGTCATAAACGAAGCCATTTTCAGT
  GCCAATCGACTCACGGGTTCAGATGTGAATTTGGTGGCTGAAAACCTCTTGTCCCCGGAGGACATTGTCC
  TGTTCCACAAGGTCACACAGCCTAGAGAAGTCGACACTGTACTGACCACCCAGGGGACATCCGCCGTCCG
  GCCTGTGGTCACCGCATCAGCTACCAGGCCACCGAAGCACAGTGAGGATCTCTCAGCTCCCAGTACTCCT
  AGGCAGCCTGTGGACACCCCAGGGCTCAGCACAGTGGCGTCAGTGACAGTGTCCCACCAAGTCCAGGGTG
  ACATGGCTGGCAGAGGGAATGAGGAGCAGCCACATGGTATGAGGTTCCTGTCCATCTTCTTCCCTATTGC
  ACTGGTTGCCCTCCTTGTCCTTGGGGCCGTCCTGCTGTGGAGGAACTGGCGGCTGAAGAACATCAACAGC
  ATAAACTTTGACAACCCAGTCTACCAGAAGACCACAGAGGACGAGCTCCACATTTGCCGAAGCCAGGATG
  GCTATACCTACCCCTCAAGACAGATGGTCAGCCTGGAGGACGATGTGGCATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001252658
• Insert Size: 2433 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001252658.1, NP_001239587.1
• RefSeq Size: 4393 bp
• RefSeq ORF: 2433 bp
• Locus ID: 16835
• UniProt ID: P35951
• Cytogenetics: 9 7.87 cM
• Gene Summary: Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an in-frame exon in the 3' coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.