Fgfr3 (NM_001205270) Mouse Untagged Clone

CAT#: MC228968

Fgfr3 (untagged) - Mouse fibroblast growth factor receptor 3 (Fgfr3), transcript variant 5

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Fgfr3
• Synonyms: CD333; Fgfr-; Fgfr-3; Flg-2; FR3; HBGF; HBGFR; Mfr3; sa; sam3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228968 representing NM_001205270
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTAGTCCCGGCCTGCGTGCTAGTGTTCTGCGTGGCGGTCGTGGCTGGAGCTACTTCCGAGCCTCCTG
  GTCCAGAGCAGCGAGTTGTGCGGAGAGCGGCAGAGGTTCCAGGGCCTGAACCTAGCCAGCAGGAGCAGGT
  GGCCTTCGGCAGTGGGGACACCGTGGAGCTGAGCTGCCATCCTCCTGGAGGTGCCCCCACAGGGCCCACG
  GTCTGGGCTAAGGATGGTACAGGTCTGGTGGCCTCCCACCGCATCCTGGTGGGGCCTCAGAGGCTGCAAG
  TGCTAAATGCCTCCCACGAAGATGCAGGGGTCTACAGCTGCCAGCACCGGCTCACTCGGCGTGTGCTGTG
  CCACTTCAGTGTGCGTGTAACAGATGCTCCATCCTCAGGAGATGACGAAGATGGGGAGGACGTGGCTGAA
  GACACAGGGGCTCCTTATTGGACTCGCCCGGAGCGAATGGATAAGAAACTGCTGGCTGTGCCAGCCGCAA
  ACACTGTCCGCTTCCGCTGCCCAGCTGCTGGCAACCCTACCCCCTCCATCTCCTGGCTGAAGAATGGCAA
  AGAATTCCGAGGGGAGCATCGCATTGGGGGCATCAAGCTCCGGCACCAGCAGTGGAGCTTGGTCATGGAA
  AGTGTGGTACCCTCCGATCGTGGCAACTATACCTGTGTAGTTGAGAACAAGTTTGGCAGCATCCGGCAGA
  CATACACACTGGATGTGCTGGAGCGCTCCCCACACCGGCCCATCCTGCAGGCTGGGCTGCCGGCCAACCA
  GACAGCCATTCTAGGCAGTGACGTGGAGTTCCACTGCAAGGTGTACAGCGATGCACAGCCACACATCCAG
  TGGCTGAAGCACGTGGAAGTGAACGGCAGCAAGGTGGGCCCTGACGGCACGCCCTACGTCACTGTACTCA
  AGACTGCAGGCGCTAACACCACCGACAAGGAGCTAGAGGTTCTGTCCTTGCACAATGTCACCTTTGAGGA
  CGCGGGGGAGTACACCTGCCTGGCGGGCAATTCTATTGGGTTTTCCCATCACTCTGCGTGGCTGGTGGTG
  CTGCCAGCTGAGGAGGAGCTGATGGAAACTGATGAGGCTGGCAGCGTGTACGCAGGCGTCCTCAGCTACG
  GGGTGGTCTTCTTCCTCTTCATCCTGGTGGTGGCAGCTGTGATACTCTGCCGCCTGCGCAGTCCCCCAAA
  GAAGGGCTTGGGCTCGCCCACCGTGCACAAGGTCTCTCGCTTCCCGCTTAAGCGACAGCAGGTGTCCTTG
  GAATCTAACTCCTCTATGAACTCCAACACACCCCTTGTCCGGATTGCCCGGCTGTCCTCAGGAGAAGGTC
  CTGTTCTGGCCAATGTTTCTGAACTTGAGCTGCCTGCTGACCCCAAGTGGGAGCTATCCAGGACCCGGCT
  GACACTTGGTAAGCCTCTTGGAGAAGGCTGCTTTGGACAGGTGGTCATGGCAGAAGCTATTGGCATCGAC
  AAGGACCGTACTGCCAAGCCTGTCACCGTGGCCGTGAAGATGCTGAAAGATGATGCGACTGACAAGGACC
  TGTCGGACCTGGTATCTGAGATGGAGATGATGAAAATGATTGGCAAGCACAAGAACATCATTAACCTGCT
  GGGGGCGTGCACACAGGGTGGGCCCCTGTATGTGCTGGTGGAGTACGCAGCCAAGGGCAATCTCCGGGAG
  TTCCTTCGGGCGCGGCGGCCTCCAGGCATGGACTACTCCTTTGATGCCTGCAGGCTGCCAGAGGAACAGC
  TCACCTGCAAGGATCTAGTGTCCTGTGCCTACCAGGTGGCACGGGGCATGGAATACTTGGCTTCTCAGAA
  GTGTATTCACAGAGACTTGGCTGCCAGAAACGTCCTGGTGACCGAGGACAATGTGATGAAGATTGCGGAC
  TTTGGCCTGGCTCGAGATGTGCACAACCTGGACTACTACAAGAAGACCACAAATGGCCGGCTACCTGTGA
  AGTGGATGGCACCAGAGGCCCTTTTTGACCGAGTCTACACCCACCAGAGTGATGTTTGGTCTTTTGGTGT
  CCTCCTCTGGGAGATCTTTACGCTGGGGGGCTCACCGTATCCTGGCATCCCAGTGGAAGAGCTTTTCAAG
  CTGTTGAAAGAGGGCCACCGCATGGACAAGCCAGCCAGCTGCACACATGACCTGTACATGATCATGCGGG
  AATGTTGGCATGCGGTGCCTTCACAGAGGCCCACCTTCAAGCAGTTGGTAGAGGATTTAGACCGCATCCT
  CACTGTGACATCAACCGACGAGTACTTGGACCTCTCCGTGCCGTTTGAGCAGTACTCGCCAGGTGGCCAG
  GACACGCCTAGCTCCAGCTCGTCCGGAGATGACTCGGTGTTCACCCATGACCTGCTACCCCCAGGTCCAC
  CCAGTAACGGGGGACCTCGGACGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001205270
• Insert Size: 2406 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001205270.1, NP_001192199.1
• RefSeq Size: 4222 bp
• RefSeq ORF: 2406 bp
• Locus ID: 14184
• Cytogenetics: 5 17.83 cM
• Gene Summary: This gene encodes a member of the fibroblast growth factor receptor family. Members of this family are highly conserved proteins that differ from one another in their ligand affinities and tissue distribution. A representative protein consists of an extracellular region composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment, and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This family member binds acidic and basic fibroblast growth hormone and plays a role in bone development and maintenance. Mutations in this gene may be associated with craniosynostosis and multiple types of skeletal dysplasia. A pseudogene of this gene is located on chromosome 1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Apr 2011]
  Transcript Variant: This variant (5) uses an alternate in-frame splice site in the coding region, compared to variant 1. The encoded isoform (4) is longer than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.