Eml1 (NM_001286346) Mouse Untagged Clone

CAT#: MC228967

Eml1 (untagged) - Mouse echinoderm microtubule associated protein like 1 (Eml1), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Eml1
• Synonyms: 1110008N23Rik; A930030P13Rik; AA171013; AI847476; AI853955; ELP79; EMAP; EMAPL; heco
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228967 representing NM_001286346
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGACGGCGAGGGCCCCTCAGCTGATGACAGCGCGTCTGCTGCCAGCAGCATGGAGGTGTCAGACC
  GCATCGCCTCTCTGGAGCAGCGCGTCCAGATGCAGGAGGATGACATTCAACTGCTCAAGTCAGCGCTGGC
  CGACGTGGTTCGGAGGCTGAACATCACGGAGGAGCAACAGGCTGTGCTCAACAGGAAAGGACCTACCAAA
  GCGAGGCCACTGGGGCAGACCCTGCCATTGAGAACCACCGTCAACAATGGCACCGTGTTACCAAAGAAAC
  CCAGTGCCTCCCTCCCGGCACCCTCCGGGGCCAGGAAAGAAGTAGTTGTGCCGGTAACCAAAAGCATCAA
  TAGGACCAGCTCTTCCGAAAGAGTGTCTCCAGGTGGCCGGAGGGAGAGCTCTGGGGACTCCAAAGGAAGC
  CGGAACCGCACGGGCTCAACCAGTAGCTCCTCCAGCGGCAAGAAGAACAGTGAGAGCAAACCCAAGGAGC
  CCGCATTCAGTCCAGAAGAAGGATATGTAAAAATGTTTCTTCGAGGCCGTCCGGTCACCATGTACATGCC
  CAAAGACCAAGTGGATTCGTACAGTTTGGAAGCAAAAGCTGAGTTACCAACGAAGCGGCTGAAACTGGAG
  TGGGTCTACGGGTACCGGGGGCGAGACTGTCGCAATAACTTGTACTTGCTCCCGACCGGGGAGACCGTGT
  ACTTCATTGCGTCTGTCGTGGTGCTTTACAATGTGGAGGAGCAGCTGCAGAGGCATTACGCGGGCCACAA
  CGATGACGTCAAGTGCCTAGCGGTCCATCCTGACAGGATCACCATAGCAACGGGACAAGTGGCGGGCACA
  TCTAAGGATGGAAAGCAACTGCCACCACATGTGCGCATCTGGGACTCTGTGACACTGAACACTCTGCATG
  TCATTGGAATAGGCTTTTTTGACCGGGCTGTCACCTGCATCGCATTCTCAAAGTCTAACGGAGGAGGCCA
  TCTCTGTGCTGTGGATGATTCCAACGATCACGTGCTGTCCGTGTGGGATTGGCAGAAAGAAGAGAGACTG
  GCCGACGTGAAGTGTTCGAACGAAGCGGTATTTGCGGCGGACTTCCACCCCACAGACACCAATATCATAG
  TCACCTGCGGGAAGTCACATCTCTACTTTTGGACCCTAGAAGGAAATTCCCTTAACAAGAAGCAAGGGTT
  GTTTGAGAAACAAGAGAAGCCAAAGTTTGTTCTCTGCGTGACGTTTTCTGAAAATGGCGACACCATTACT
  GGAGATTCAAGCGGCAACATCTTGGTGTGGGGGAAAGGTACAAATCGGATAAGCTATGCAGTTCAAGGGG
  CCCACGAGGGCGGCATTTTTGCACTTTGTATGCTGAGAGACGGGACGCTGGTGTCCGGAGGAGGGAAGGA
  CCGGCGACTCATTTCCTGGAACGGAAACTATCAAAAACTTCACAAAGCAGAGATTCCTGAGCAGTTTGGC
  CCAATACGGACAGTAGCCGAAGGGAAGGGCAACGTCATCTTGATAGGCACTACTAGAAACTTTGTCCTGC
  AAGGCACCTTGTCAGGGGACTTCACACCCATCACTCAGGGCCACACCGATGAGCTCTGGGGGCTAGCCAT
  CCATGCCTCCAAGCCTCAGTTCCTGACTTGTGGACATGACAAACATGCCACTCTCTGGGACGCTGTCGGT
  CACCGGCCAGTCTGGGACAAAATCATAGAGGATCCAGCTCAGTCCTCTGGTTTTCATCCTTCCGGGTCTG
  TGGTTGCAGTGGGGACACTGACTGGGAGGTGGTTTGTGTTTGACACGGAGACAAAGGACTTGGTCACCGT
  CCACACGGATGGGAATGAGCAGCTGTCCGTGATGCGGTATTCTCCAGATGGGAACTTCTTAGCAATCGGC
  TCCCATGACAACTGCATCTACATATATGGAGTTACCGACAATGGAAGGAAGTACACACGAGTTGGCAAGT
  GCTCCGGCCATTCCAGCTTCATCACCCACTTGGACTGGTCCGTGAACTCACAATTCCTGGTGTCAAATTC
  CGGGGACTACGAGATCCTCTACTGGGTTCCGTCTGCCTGTAAGCAAGTCGTGAGTGTGGAAACCACAAGG
  GACATCGAGTGGGCCACCTATACCTGCACCTTGGGATTCCACGTCTTTGGAGTGTGGCCGGAGGGCTCCG
  ATGGGACAGACATCAACGCCGTCTGCCGGGCTCACGAGAGAAAGCTCTTGTGCACAGGCGATGACTTCGG
  CAAAGTGCACCTCTTCTCATACCCGTGCTCACAGTTCCGGGCTCCAAGCCACATCTACAGTGGACACAGC
  AGCCACGTCACCAACGTGGACTTCCTCTGTGAAGACAGCCACCTTATCTCCACGGGTGGGAAAGACACAA
  GCATCATGCAGTGGCGAGTCATTTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001286346
• Insert Size: 2406 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001286346.1, NP_001273275.1
• RefSeq Size: 3943 bp
• RefSeq ORF: 2406 bp
• Locus ID: 68519
• UniProt ID: Q05BC3
• Cytogenetics: 12 59.46 cM
• Gene Summary: Modulates the assembly and organization of the microtubule cytoskeleton, and probably plays a role in regulating the orientation of the mitotic spindle and the orientation of the plane of cell division. Required for normal proliferation of neuronal progenitor cells in the developing brain and for normal brain development. Does not affect neuron migration per se.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) has an alternate 5' terminal exon, compared to variant 1. The resulting isoform (3) has a shorter and distinct N-terminus, compared to isoform 1.