Per3 (NM_001289878) Mouse Untagged Clone

CAT#: MC228965

Per3 (untagged) - Mouse period circadian clock 3 (Per3), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Per3
• Synonyms: 2810049O06Rik; mPer3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228965 representing NM_001289878
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTGCCATACACCAAAAAGTTTTAAAGTATGCCGGCCACCCTCCGTTTGAACACTCGCCCGTCAGAT
  TCTGCACTCAGAACGGAGAGTATGTCATTCTGGATTCCAGCTGGTCCAGCTTTGTCAACCCCTGGAGCCG
  GAAGGTCTCCTTCATCATTGGTCGACATAAAGTCCGAACGAGTCCATTAAATGAAGATGTTTTTGCCACC
  AGAATAAAAAAGGCAGCCAGTAACGACAAAGACATAGCAGAATTACAAGAACAAATTCACAAACTTCTCT
  TGCAGCCGGTTCATGCTAGTGCTTCCAGTGGCTACGGGAGCCTGGGCAGCAGCGGCTCACAGGAGCAGCA
  CGTCAGCATCACCTCTTCGAGTGAGTCCAGCGGGCACTGTCCGGAGGAAGGCCAGCATGAGCAGATGACC
  CTGCAGCAGGTCTATGCCAGTGTAAACAAAATTAAGAATGTGGGCCAACAGCTCTACATCGAGTCCATGG
  CCAGATCATCAGTGAAGCCAGTGGCAGAGACGTGCGTGGAACCGCAGGGTGGTGATGAGCAGAAGGACTT
  TTCTTCCTCTCAGACACTGAAAAATAAAAGCACCACGGATACTGGCTCCGGTGGCAATCTGCAGCAAGAG
  CAGCCCAGCTCGTCCTATCAGCAGATGAACTGTATCGACAGTGTCATCAGGTACCTGACAAGCTACAGCC
  TCCCGGCCTTGAAAAGAAAGTGCATCTCCTGCACAAACACATCTTCATCCTCAGAAGAAGCCAAGCCAAT
  CCCGGAGGTGGACAGCAGCCAGAGAGACACGGAACAGCTCCTGGACATACGGAAACAGGAAACAACTGGA
  CCATCCACAGACATCGAAGGAGGTGCTGCTCGGACCCTGTCCACCGCCGCACTGAGCGTGGCGTCTGGCA
  TCAGCCAGTGCAGCTGCAGCAGCACCTCTGGCCACGCTCCGCCCCTACAGTCAGCAGAAAGTGTTGCCGT
  GGCGTGTAAGCCGTGGGCCCTGAGAACGAAGGCCTCTCACCTGGCTGCAGGAGGATTTAAGCACGTGGGG
  CTCACAGCAGCTGTCCTCTCTGCACACACACAGAAGGAAGAGCAGAACTACGTTGACAGGTTCCGGGAAA
  AGATCCTGACCTCGCCCTACGGTTGCTATCTTCAGCAAGAGAGCAGAAACCGTGCTCAGTACTCCTGTGT
  TCAAGCAGGGTCCACTGCTAAGCACAGCAGATGTGCTGGAAGCGAGAGGCAGAAGCACAAACGAAAGAAG
  TTGCCAGCACCTGTGGACACCAGCAGCCCCGGTGCCCACCTCTGTCCCCATGTCACAGGACTCCTCCCGG
  ATGAGCAGCACTGGGGCCCATCCGCTAGCCCCTCCCCCCTCGGCGCAGGCTTAGCATTCCCCTCGGCCCT
  GGTAGTTCCCAGCCAGACCCCTTATCTCCTCCCCTCTTTTCCCCTCCAAGATATGGCCTCTCAGGGAGTG
  GGGGTCTCGGCAGCCTGGGGAGCTGCAGCCGGATGTCCACCTCTGTCCGCCGGCCCCCAGGCTGTTGCCG
  CGTTCCCCTCCGCTTACGTGGATACTTTGATGACCATCTTCCTGCACAACGCCCCTCTCTTCCCTCTGTG
  GCCGCCCTCGTTCTCCCCATACCCATCCCTGGGGGCCGCAGGGTCTTCTGAACTGGCACCCTTAGTACCA
  GCAATGGCTCCAAACCCGGAACCAACCACTTCAGGCCACAGCCAAAGGAGAGTGGAGGAGAACTGGGAGG
  CACACAGTGAAGAGCTTCCGTTCATTAGCTCACGGAGCAGTTCACCGTTACAGTTAAATTTACTCCAGGA
  AGAAATGCCTGCGCCGTCAGAGTCCGCAGACGCAGTGAGAAGAGGCGCTGGGCCAGACGCTAAGCATCAC
  TGTGTTACAGGTCCCAGTGGCAGTAGGAGCCGTCACTGCACCTCTGGTGAGCTGGCCACGGCAACAGCGC
  AGCAGGAGTCTGCTGCTGCCTCAGGAAGCAGTGCCAGCAGTATATACTTCAGTAGCACTGACTATGCTTC
  TGAAGTCTCTGAAAACAGACAGAGGCCACAGGATAGACAGAGAGACGAAGCCCTTCCCGGGGCGGCTGAA
  GAGTCCATCTGGAGAATGATAGAGCGGACACCAGAGTGTGTTCTCATGACATACCAGGTGCCCGAGAGGG
  GTCGAGAGGAGGTGCTGAAGCAGGACCTGGAGAAGCTCCAGAGCATGGAACAGCAGCAGCCCCTGTTCTC
  TCCCGCGCAGAGGGAGGAGCTGGCCAAGGTGCGCTCCTGGATCCACAGCCACACAGCCCCTCAGGAGGGA
  CACCTCCAGAGCTGTGTCGCCTGTGAAGACAGAGGTTCAGTGGGTGACACTGCAGAGGTCCTGGAACAGC
  ACCCAGCAGAAGACACCAGTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001289878
• Insert Size: 2403 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289878.1, NP_001276807.1
• RefSeq Size: 6048 bp
• RefSeq ORF: 2403 bp
• Locus ID: 18628
• Cytogenetics: 4 E2
• Gene Summary: This gene is a member of the Period family of genes and is expressed in a circadian pattern in the suprachiasmatic nucleus, the primary circadian pacemaker in the mammalian brain. Genes in this family encode components of the circadian rhythms of locomotor activity, metabolism, and behavior. This gene is upregulated by Clock/Arntl heterodimers but then represses this upregulation in a feedback loop using Per/Cry heterodimers to interact with Clock/Arntl. Polymorphisms in this gene have been linked to sleep disorders. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2014]
  Transcript Variant: This variant (3) contains an alternate exon and uses a downstream start codon compared to variant 1. The resulting isoform (3) is shorter at the N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.