Thsd1 (NM_001293688) Mouse Untagged Clone

CAT#: MC228958

Thsd1 (untagged) - Mouse thrombospondin, type I, domain 1 (Thsd1), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Thsd1
• Synonyms: 4833423O18Rik; Tmtsp
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228958 representing NM_001293688
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAACCCATGTTGAAAGACTTTTCAAATCTCTTGCTGGTGGTGCTCTGTGACTATGTCCTCGGAGAAG
  CCGAATACCTCCTCCTCCAAGAGCCAGTCCATGTGGCACTGAGCGACAGAACGGTGTCAGTGGGTTTCCA
  CTACCTCAGTGACGTCAACGGGACACTGAGGAATGTGTCTGTCATGCTGTGGGAGGCCAACACCAATCGG
  ACTCTTACCACTAAGTACCTCCTGACCAACCAGGCCCAAGGAACACTCCAGTTTGAATGTTTCTACTTCA
  AAGAGGCTGGTGACTACTGGTTTGTAATGATCCCGGAAGTGACAGACAATGGCACGCAAGTTCCACTCTG
  GGAGAAAAGTGCCTTTCTGAAGGTAGAATGGCCTGTCTTTCACATTGATTTAAATAGGACAGCCAAGGCA
  GCAGAAGGCACCTTTCAAGTGGGTGTTTTTACCACCCAACCGCTCTGCCTGTTTCCCGTGGACAAGCCAG
  ACATGCTGGTGGATGTTATTTTCACTGACCGTCTTCCGGAGGCAAGAGCAAGTTTGGGACAGCCGCTGGA
  GATCAGAGCCAGCAAAAGGACAAAACTCACTCAAGGTCAGTGGGTCGAGTTTGGCTGTGCACCGGTAGGG
  GTGGAAGCCTACGTTACAGTCATGCTGAGGCTGTTGGGTCAAGACTCAGTCATTGCTTCTACGGGACCTA
  TTGACCTGGCTCAAAAATTCGGATACAAATTGATGATGGCACCGGAAGTCACGTGTGAGTCTGTGCTGGA
  GGTGATGGTACTGCCACCTCCTTGTGTCTTCGTCCAAGGAGTCCTGGCTGTTTACAAAGAAGCCCCCAAA
  CGCCCGGAGGAGAGGACTTTCCAGGTGGCTGAAAACAGACTGCCCCTGGGAGAGAGGAGAACGGTGTTCA
  ACTGCACTTTATTTGATGTAGGGAAGAACAAATACTGTTTTAACTTTGGAATTGTGAAGAAAGGCCATTT
  TTCTGCAAAGGAATGCATGCTAATTCAGAGAAATATAGTTTTCCGGCCACCAGGCCCATCGCCTGTTTCA
  CCCCAGGACCCTGTGAAGTCCAACAACGTGGTGACCGTCACAGGGATCTCCCTGTGCCTGTTCATCATCT
  TTGCCACGGTGCTCATCACTCTCTGGAGGAGGTTTGGCCGAGCCCCCAAATGCAGCACGCCCGTTCGCCA
  CAACTCCATCCATTCCCCTGGCTTCCGGAAGAACTCCGATGAAGAGAACATCTGCGAGCTGAGTGAGCCT
  CGCGGAAGCTTCTCGGATGCCGGTGACGGACCTAGGGGAAGCCCAGGGGACACGGGCATCCCATTGACTT
  ACAGGTGCAGTGCATCAGCGCCTCCTGAGGATGAGGCCTCGGGCAGTGAGAGCTTCCAGTCCAACGCTCA
  GAAGATCATCCCGCCCTTGTTTAGCTACCGCCTTGCCCAGCAGCAGCTGAAGGAGATGAAGAAGAAAGGG
  CTGACCGAGACCACCAAAGTGTACCACGTATCTCAAAGCCCCCTGACAGACACTGTAGTGGATGCCACGG
  CCAGCCCTCCCTTAGACCTGGAATGCCCCGAAGAGGCTGCAGCAAGCAAGTTCCGAATCAAATCTCCATT
  TCTGGACCAGCCTGGGGCAGGTACCGGGGAAAGGCCTCCCTCCAGGCTGGATGGCATCGTGCCTCCTCCT
  GGCTGTGCGGTCAGTCCCAGCCAGACCCTGATCCGAAAGTCACAGATAAGGTCGACCGGTGGCAGAGATG
  GCTCATCGGAGAGGTGCCACTCCAGAAGTTCCCTCTTCAGGAGGACTGCTAGTTTTCATGAAACCAAGCA
  GTCTCGCCCTTTCCGGGAGAGGAGTTTGTCAGCCCTGACTCCCCGCCAGGTCCCCGCCTACAGTTCCAGG
  ATGCGGACCTGGGACCAGATGGAGGATAGATGTCGGCCTCCCAGTCGAAGTACCCACCTGCTTCCAGAGA
  GACCAGAGCACTTCCAAGGGGCAGGTCGGACCAGCAGTCCTTTGGGTCCACTCTCCAAATCCTACACTGT
  GGGGCATCCCAGGAGGAAACCGGACCCAGGGGATCGTCAGGCCGGATTGGTGGCAGGAGCTGAGAAAATG
  GAGCCTCACCGAGCTCACAGGGGACCGTCCCCCAGTCACAGGAGTGCCTCAAGGAAGCAGTCTTCCCCCA
  TTTTCCTCAAAGATAGCTACCAGAAAGTCAGTCAGCTTAGCCCTTCTCACTTCAGAAAAGATAAATGCCA
  GAGCTTCCCCATCCACCCCGAGTTCGCCTTCTATGACAATACCTCTTTCCGCCTCACCGAGGCTGAGCAG
  AGAATGCTGGACCTCCCAGGATACTTCGGCTCCAACGAAGAGGACGAAACCACAAGTACACTCAGTGTGG
  AGAAGTTAGTGATCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001293688
• Insert Size: 2397 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001293688.1, NP_001280617.1
• RefSeq Size: 4273 bp
• RefSeq ORF: 2397 bp
• Locus ID: 56229
• Cytogenetics: 8 A2
• Gene Summary: Is a positive regulator of nascent focal adhesion assembly, involved in the modulation of endothelial cell attachment to the extracellular matrix.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (3) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.