Prdm4 (NM_001302886) Mouse Untagged Clone

CAT#: MC228951

Prdm4 (untagged) - Mouse PR domain containing 4 (Prdm4), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Prdm4
• Synonyms: 1700031E19Rik; 2810470D21Rik; AW552272; SC; SC-; SC-1; SC1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228951 representing NM_001302886
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATGACATGAACTTGAGCCCAGTGGGGATGGAGCAGCTGTCTTCATCCTCTGTGAGCAATGCCCTGC
  CAGTCTCAGGAAGTCACCTGGGCTTGGCTGCCTCTCCCTCTCACAGTGCCATCCCTGCCCCAGGACTTCC
  AGTGGCAATTCCAAACCTGGGTCCCTCCCTGAGCTCTCTGCCTTCTGCCTTGTCTCTTATGCTCCCCGTG
  GGCATTGGAGACCGAGGAGTGATGTGTGGGCTACCTGAGAGGAACTACACCCTACCTCCACCTCCTTACC
  CTCACCTAGAGAGCAGTTACTTCAGAACCATTCTACCTGGCATTTTATCTTATTTAGCTGACAGACCACC
  TCCTCAGTATATCCACCCCAACTCTATAAATGTTGATGGAAATACAGCATTATCTATCACCAACAACCCT
  TCAGCACTAGATCCGTATCAGGCCAATGGAAATGTTGGATTAGAACTAGGCATTGTTTCAATAGACTCTC
  GCTCTGTAAACACACATGGTGCCCAAAGTCTTCATCCTAATGATGGCCACGAAGTAGCATTGGACACAAC
  AATCACCATGGAGAATGTTTCTAGGGTTACCAGCCCAATCTCCACAGATGGAATGGCAGAGGAGCTTACA
  ATGGACGGTGTTACAGGAGAGCATCCCCAAATCCCAAATGGCTCCAGAAGTCATGAGCCTCTCTCTGTGG
  ATTCTGTGAGCAACAGCCTTACAGCAGAAGCTGTAGGACATGGTGGTGTGATACCCATTCACGGGAATGG
  TCTGGAGCTTCCTGTGGTCATGGAAACTGACCACATTGCAAACCGGGTCAATGGGATGTCTGACAGTACC
  CTCAGTGACTCCATCCACACCGTGGCCATGAGCACCAACTCTGTAAGCGTGGCACTCTCTACCTCACACA
  ACCTGGCCTCCCTAGAGTCTGTTTCCCTCCATGAAGTTGGCCTTAGCCTAGAGCCTGTGGCTGTCTCTTC
  TATCACGCAGGAGGTTGCCATGGGGACAGGTCATGTAGATGTGTCCTCAGACAGCTTGTCTTTTGTACCA
  TCTTCACTGCAAATGGAAGACTCCAATTCAAACAAGGAAAACATGGCAACCTTGTTTACAATTTGGTGCA
  CTCTTTGTGACCGAGCCTACCCCTCAGATTGCCCTGATCATGGACCAGTGACTTTTGTTCCTGACACACC
  AATAGAGAGCAGAGCAAGACTATCCCTCCCGAAGCAGCTTGTTCTCCGCCAGTCCATCGTGGGAACAGAT
  GTTGGTGTATGGACTGCAGAAACCATTCCTGTGCGGACTTGCTTCGGACCTCTAATTGGTCAGCAGAGTC
  ACTCCTTGGAAGTAGCAGAGTGGACAGACAAGGCAGTTAACCATGTCTGGAAGATATACCACACTGGTGT
  CCTGGAGTTCTGTATCATTACAACCGATGAGAATGAGTGTAATTGGATGATGTTTGTGCGCAAAGCCAGG
  AACCGTGAAGAACAGAATTTGGTGGCCTATCCCCATGATGGGAAAATCTATTTCTGTACCTCACAAGACA
  TCCCCCCTGAAAGTGAGCTGCTTTTCTATTACAGCCGGAATTACGCTCAACAGATAGGTGTTCCTGAACA
  CCCAGATGTGCACCTCTGTAACTGTGGCAAGGAGTGCAGTTCCTATTCAGAGTTCAAAGCTCATCTGACC
  AGCCATATCCACAATCATCTCCCTAGCCAGGGCCACAGCAGCAGCCATGGGCCAAGCCACAGCAAGGAAA
  GGAAGTGGAAGTGTTCAATGTGCCCCCAGGCTTTTATCTCGCCTTCCAAACTCCACGTTCACTTTATGGG
  CCACATGGGTATGAAGCCCCACAAGTGTGATTTTTGTAGCAAGGCTTTTAGTGATCCAAGCAACCTACGG
  ACACACCTCAAAATACATACAGGTCAGAAAAATTATAGGTGTACTTTGTGTGACAAGTCTTTCACGCAGA
  AGGCTCACCTGGAGTCTCACATGGTCATCCACACGGGTGAGAAGAATCTCAAGTGTGATTACTGTGACAA
  GCTGTTCATGCGGAGACAGGACCTCAAGCAGCACGTGCTCATCCACACGCAAGAACGCCAGATCAAGTGT
  CCGAAGTGTGATAAACTGTTCTTGAGAACAAACCACTTGAAGAAGCATCTCAATTCTCACGAAGGAAAAC
  GAGATTATGTCTGCGAAAAATGTACAAAGGCTTATCTAACCAAGTACCATCTCACTCGCCACCTGAAGAC
  CTGCAAAGAGCCCAGCTCCAGCTCCTCTGCACAGGAGGAAGAGGATGATGAGTCGGAGGAAGAAGATCTC
  GCAGACTCCATGAGGACAGAGGACTGCAGGATGGGCAGTGCTGTTTACTCAACAGACGAGTCTCTCTCCG
  CACATAAATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001302886
• Insert Size: 2391 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001302886.1, NP_001289815.1
• RefSeq Size: 3955 bp
• RefSeq ORF: 2391 bp
• Locus ID: 72843
• Cytogenetics: 10 C1
• Gene Summary: This gene encodes a member of the PR/SET family of zinc finger proteins. This protein has been shown to bind DNA in a sequence-specific manner and has been implicated in neural stem cell proliferation and differentiation. Pseudogenes have been identified on chromosomes 14 and X. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]
  Transcript Variant: This variant (2) contains a longer 5' UTR and uses an alternate in-frame splice site in the central coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1.