Fam160a2 (NM_001242365) Mouse Untagged Clone

CAT#: MC228944

Fam160a2 (untagged) - Mouse family with sequence similarity 160, member A2 (Fam160a2), transcript variant 4

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Fam160a2
• Synonyms: 4632419K20Rik; 6530415H11Rik; FHIP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228944 representing NM_001242365
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAAGATGAACTGGTTAAGCAGGCTGGCCTCCCGGGTCCCTGGGCACCGTGTACCACAAGGGGCCA
  GTCTACAGACCCCTGTCATGGCTGACCCTGAGACATGCCTCATGGTCTTCAAGAATCATTGGTCCCAGGT
  GGTACGAATCCTGGAGCGGCAAGGCCCTCGGGCAGCTACTGGAGGTGCAGATGATCTCAGTGCTGTGAGA
  AACCACACGTACCAAATGCTAACATTGCTGGCAGAGGATCGAGCAGTTCCTTCAGCTCCCTCAGGCCCTG
  GGCCCCTGCTAGAGTTTGCTCTTCGTGAAGATCTGCTGAGCCGAGTGTTGACATGGCAGTTGCAATGGGA
  TGAGTTTGGGGATGGGGTTGAAGAACGGCGGGCTGAACAGTTGAAACTATTTGAAATGCTAGTGAGCGAA
  GCTCGCCAACCTCTGTTGCGGCATGGGCCAGTTCGAGAGGCTCTGCTTGCCCTACTGGATGCCTGTGGCC
  GCCCTGTGCCCAGTAGTCCAGCACTGGATGAAGGCCTGGTGCTACTTCTCAGCCAACTGTGCGTGTGTGT
  TGCTCGGGAGCCTTCACTGCTCGAGTTCTTCTTGCAGCCACCCCCAGAGCCTGGAGCTGCTCCACGTCTT
  CTCCTCTTTTCTCGCCTTGTCCCCTTTGTCCATCGAGAAGGCACCCTGGGCCAGCAGGCCCGTGATGCTC
  TACTTCTGCTCATGGCTCTGTCAGATGGGAGCCCCACTGTGGGCCGCTACATCGCAGATCACTCTTACTT
  CTGCCCGGTGCTGGCCACAGGGCTGAGCGCCCTGTACTCCTCACTGCCTCGAAAGATTGAGGTTCCGGGG
  GATGATTGGCACTGCTTGCGCCGGGAAGACTGGATAGGAGTGCCTGCCCTTGCACTCTTCATGAGTTCCC
  TTGAGTTCTGCAATGCAGTCATTCAGGTAGCTCACCCGCTGGTGCAGAAGCAGCTGGTTGATTATATTCA
  CAATGGTTTCCTGGTGCCTGTCATGGGCCCTGCCCTGCACAAGACCTCTGTGGAGGAGATGATTGCGAGT
  ACCGCCTACCTGGAACTTTTCCTGCGGAGCATCTCAGAGCCTGCTTTGCTCCGTACCTTCCTGCGATTCC
  TGTTGTTACATCGGCATGATACCCACACCATCCTTGACACGCTCGTTGCCCGTATTGGCAGCAACTCCCG
  GCTCTGCATGGTCTCTCTGAGTCTCTTCAGGACCCTTCTGAACCTCAGCTGTGAGGATGTTCTGCTGCAG
  CTGGTTCTCAGGTATCTTGTCCCATGTAACCATGTGATGCTAAGCCAGAAGCCAGCTGTGCGTGATGTGG
  ATCTTTATGGACGAGCAGCTGACAAGTTTCTCTCCCTAATACCACGCTGCTGCCGGCACCATGCCCCTAG
  CCCACCTCGACCAGAGCATGCCTCATGGGCACGAGGTGGGCCTAGCAGAGAGGCAGGGAGAAGGGAGGAC
  ATCACGGGCCCTGGAAGCCCAAGTGTTGATTCTTCGTCAGTGGTAACAGTGCCCCGCCCCTCCACACCAT
  CTCGTCTGGCTCTGTTTCTTCGGCAACAGAGCCTAGGTGGCTCTGAGTCCCCAGGTCCAGTTCCTCGATC
  ACCAGGGCTTACTGCATCTCCAACTTCCAGCCCTAGTCGAAGGCCCAGCCCTGCAGAGGAGCCTGGCCCC
  TTCATGGCTGTGCTCTTTGCCAAACTCGAGAACATGCTGCAGAACTCCGTCTATGTCAACTTCCTGCTGA
  CGGGGCTGGTGGCCCAGCTGGCCTGTCACCCCCAGCCCCTGCTCCGCTCTTTCCTGCTCAACACCAACAT
  GGTCTTCCAGCCCAGTGTCAAGTCCCTGCTGCAGGTGCTGGGCTCTGTGAAGAATAAGATTGAGAGCTTT
  GCGGCCTCCCAGGAGGACTTCCCTGCTCTGCTATCCAAAGCCAAGAAGTACCTCATTGCCCGTGGCAAGC
  TGGACTGGGCTGAGGGTCCTACAGCAGGACCTGCCCCCCGCCGCTCTGATTCCCTAGTGAGGAGCCGGAG
  GCCATCCTTGGGGGAATTACTCCTGCGGCATGCACACAGCCCAACCAGGGCCCGACAGGCGGTGCAGGTT
  CTCCAACCTGGGCGAGATGGAACAGGACTTGGACTAGGTGGGGGCTCTCCAGGGGCTTCAACTCCAGTTC
  TACTTCCCCGGGGCGGGGCTTCTGAGCGCCAAGGTGAGGCTCTTCGAGTCAAGAATGCTGTCTACTGTGC
  AGTCATTTTCCCTGAGTTTCTCAAGGAGTTGGCTGCCATCTCCCAGGCCCATGCTGTCACCTCGCCTTTC
  TTGTTGGATACTTCAGAGGAGGTATCTCTTCCTCCCATCTCAGGCTTTGGGCCCCTCAATCCTTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001242365
• Insert Size: 2376 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001242365.2, NP_001229294.1
• RefSeq Size: 10301 bp
• RefSeq ORF: 2376 bp
• Locus ID: 74349
• UniProt ID: Q3U2I3
• Cytogenetics: 7 E3
• Gene Summary: Component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex) (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (4) differs in the 5' UTR and has alternate in-frame splice junctions at the 3' ends of two exons compared to variant 1. The resulting isoform (c) contains an alternate internal segment and lacks another alternate internal segment compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.