Wwp1 (NM_001276292) Mouse Untagged Clone

CAT#: MC228934

Wwp1 (untagged) - Mouse WW domain containing E3 ubiquitin protein ligase 1 (Wwp1), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Wwp1
• Synonyms: 8030445B08Rik; AIP5; SDRP1; Tiul1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228934 representing NM_001276292
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGACGAGCAGCTGATTGTAAATGTGACCCCACAGACCACCTTGGAGTTCCGAGTTTGGAGTCACCAC
  ACTTTAAAGGCAGATGCTCTATTAGGAAAAGCAACAGTGGACTTGAAACAAGTGCTGTTAACACACAACA
  GAAAATGTTGCCTGTTGAAGGTACTATTGGAATTGATAATCATGTATCAACCAATACTGTTGTTCCCAAC
  TCCTGCTGTTCACATGTAGTTAATGGAGAAAACACACCTTCATCCCCGTCTCAGGTTGCTGCCAGACCCA
  AAAATGCTCCAGCTCCAAAGCCAGTCACATCTGCGCCCACCAGTGACACGGTTAATGGAGAATCATCATC
  TGTCCTAGCTGATAATACTTCAACCATGGGTACTTTACTACCATCTGAAGATACCACCTCGACTTCCAAT
  TGCACTAGCACCACCACCCAAGAGCCTCCTGTACAAGAACCCCCAGCATCCTCAGAACACAGTGAATGTA
  TTCCCTCTGCCAGTGCGGAAGTGGGACCAGAAGCTCGGAGCCTCATAGACCCTGACTCTGATTCTAGAAA
  TAATTCTGTGTTTGACAAAGTAAGGCAGCCAGAAGGGTGTGTAGAACCTCTGCGGCCACAGTCTGGAAAC
  ACCAACACAGAAGCTTTGCCATCAGGGTGGGAACAGAGAAAAGATCCTCATGGTAGAACCTATTATGTGG
  ATCATAATACTCGAACTACTACATGGGAGAGACCACAGCCTTTGCCTCCAGGTTGGGAAAGAAGAGTTGA
  TGATCGTGGAAGAGTTTATTATGTGGATCATAACACCAGAACAACAACCTGGCAGCGGCCTACTATGGAA
  TCTGTTCGAAATTTTGAACAGTGGCAGTCTCAGCGGAATCAATTGCAGGGAGCTATGCAACAATTCAACC
  AACGATACCTCTATTCGGCTTCCATGTTAGCTGCAGAAAATGACCCCTATGGACCCTTACCACCGGGCTG
  GGAAAAAAGAGTGGATTCAACAGACAGAGTTTACTTTGTGAATCACAATACAAAAACAACCCAGTGGGAA
  GATCCAAGAACTCAAGGCTTACCAAATGAAGAACCCCTGCCCGAAGGCTGGGAAATCAGGTATACTCGGG
  AAGGTGTTAGGTACTTTGTTGATCATAACACGAGAACAACAACATTCAAAGACCCTCGCAATGGGAAGTC
  ATCAGTAACTAAAGGTGGTCCACAGATTGCTTATGAACGCAGCTTTAGGTGGAAGCTTGCTCACTTCCGT
  TACTTGTGCCAGTCTAATGCACTACCGAGTCATGTAAAGATCAATGTGTCCCGACAGACATTATTTGAAG
  ATTCCTTCCAACAGATTATGGCATTAAAACCATATGACTTGAGGAGGCGATTATATGTAATATTTAGAGG
  AGAAGAAGGTCTTGATTATGGTGGCCTTGCAAGAGAATGGTTTTTCTTGCTTTCCCATGAAGTTTTGAAC
  CCAATGTATTGCTTATTTGAGTATGCAGGCAAGAATAACTATTGTTTACAGATAAATCCAGCATCAACTA
  TAAATCCTGACCATCTTTCATACTTCTGTTTTATTGGTCGCTTTATTGCAATGGCACTGTTCCATGGAAA
  GTTTATTGATACTGGTTTCTCTTTACCATTCTACAAGCGTATGCTAAGTAAAAAACTTACTATTAAGGAT
  TTGGAATCTATTGATACTGAATTTTACAACTCTCTAATCTGGATAAGAGATAACAATATTGAAGAATGTG
  GCTTAGAAATGTACTTTTCTGTAGACATGGAGATTCTGGGAAAAGTTACTTCACATGATTTAAAGTTGGG
  AGGTTCCAATATCCTGGTGACGGAAGAAAACAAAGATGAATATATTGGTTTAATGACAGAATGGCGTTTT
  TCTCGAGGAGTACAAGAACAGACCAAAGCTTTCCTTGATGGGTTTAATGAAGTTGTTCCCCTTCAGTGGC
  TACAGTACTTTGATGAAAAAGAATTGGAGGTTATGCTGTGTGGCATGCAAGAGGTTGACTTGGCAGACTG
  GCAGAGAAATACTGTTTATCGACATTATACAAGAAACAGCAAGCAAATTATTTGGTTTTGGCAGTTTGTG
  AAAGAGACTGATAATGAAGTACGAATGCGACTCTTGCAGTTTGTGACTGGAACCTGTCGCTTGCCTCTAG
  GAGGATTTGCTGAGCTCATGGGAAGTAATGGGCCTCAAAAGTTCTGTATTGAAAAAGTTGGCAAAGACAC
  CTGGTTACCAAGAAGCCATACATGTTTTAATCGCTTGGATCTACCACCATATAAGAGTTATGAACAACTA
  AAGGAAAAACTTCTTTTTGCAATAGAAGAGACAGAGGGATTTGGACAAGAATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001276292
• Insert Size: 2364 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001276292.1, NP_001263221.1
• RefSeq Size: 6296 bp
• RefSeq ORF: 2364 bp
• Locus ID: 107568
• UniProt ID: Q8BZZ3
• Cytogenetics: 4 A3
• Gene Summary: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Ubiquitinates and promotes degradation of SMAD2 in response to TGF-beta signaling, which requires interaction with TGIF (By similarity). Ubiquitinates ERBB4 isoforms JM-A CYT-1 and JM-B CYT-1, KLF2, KLF5 and TP63 and promotes their proteasomal degradation. Ubiquitinates RNF11 without targeting it for degradation. Ubiquitinates and promotes degradation of TGFBR1; the ubiquitination is enhanced by SMAD7. Ubiquitinates SMAD6 and SMAD7.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) has multiple coding region differences and initiates translation at a downstream start codon, compared to variant 1. The use of the start codon used in variant 1 would render the transcript a candidate for nonsense-mediated mRNA decay (NMD). Leaky scanning may allow translation initiation at the downstream AUG, which is associated with a strong Kozak sequence. The resulting isoform (2) is shorter and has a distinct N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.