Pfkp (NM_001291071) Mouse Untagged Clone

CAT#: MC228930

Pfkp (untagged) - Mouse phosphofructokinase, platelet (Pfkp), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pfkp
• Synonyms: 1200015H23Rik; 9330125N24Rik; ATP-PFK; PFK-C; PFK-P
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228930 representing NM_001291071
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGACTTGGATTCTTCGAGCTCCAGCGCCTATCCGAAGTACCTGGAGCACCTCTCTGGGGATGGCA
  AAGCTATCGGTGTCCTGACCAGCGGCGGGGATGCCCAAGGCATGAATGCTGCTGTCCGCGCTGTGGTGCG
  CATGGGAATCTACACGGGGGCCAAAGTGTACTTTATATATGAGGGTTACCAAGGCTTGGTGGATGGAGGC
  TCCAATATTGTGGAAGCCAAATGGGACTGTGTCTCCAGCATTCTGCAAGTGGGTGGGACCATCATCGGTA
  GTGCGCGCTGCAAAGCCTTTCGAAGCCGTGAAGGGCGCCTGAAAGCTGCCTGTAACTTGGCGCGCTTGGG
  CATAACCAATCTGTGCGTGATCGGCGGGGACGGAAGTCTCACGGGAGCCAACCTCTTCCGAAAGGAGTGG
  AGCGGACTTCTGGAAGAGCTGGCTCGGAATGGTGATATCGATAACGACACAGTGCAGAAGTATTCCTACC
  TCAACGTGGTGGGTATGGTGGGCTCCATTGACAATGACTTCTGTGGCACAGACATGACCATTGGTACAGA
  TTCAGCCCTGCACCGAATTATTGAAGTTGTTGATGCCATCATGACCACTGCTCAGAGCCACCAGAGGACC
  TTCGTCCTGGAGGTGATGGGGAGACACTGTGGTTACTTGGCCTTGGTGAGCGCCTTGACTTGCGGTGCCG
  ACTGGGTGTTCCTTCCGGAGTCTCCACCTGAGGAAGATTGGGAGGAAAATATGTGCCTCAAACTCTCAGA
  GAACCGAGCCCGAAAAAAGAGGCTGAATATCATCATTGTGTCTGAAGGAGCAATCGACATGCAAAATAAG
  CCAATCACCTCTGAGAAAATCAAGGAGCTTGTAGTGAAGAATTTGGGATTTGACACCCGGGTCACCATCC
  TTGGGCATGTCCAACGAGGAGGGACCCCATCTGCATTTGACAGGATTTTGGCCAGCCGTATGGGAGTGGA
  GGCTGTCATTGCCTTGCTGGAAGCTACCCCCGAGACCCCAGCCTGTGTCGTGTCACTAAGAGGAAACCAA
  GCAGTGCGCCTGCCACTGATGGAGTGTGTGCAAATGACCCAGGATGTACAGAAGGCAATGGATGAAAGAA
  GATTTAAGGAAGCCGTGAAACTCCGAGGAAGGCGTTTTGAGGGAAACCTGAACACCTATAAGCGTCTTGC
  CATTAAACTGCCTGATGAGAAGATTGTCAAGAGCAACTGCAATGTAGCCGTCATCAATGTAGGGGCACCT
  GCTGCCGGAATGAATGCAGCCGTGCGCTCTGCTGTTCGAGTCGGGATTGCAGACGGCCACAAGATGTTTG
  CAATCTATGACGGCTTTGAAGGATTCGCCAATGGCCAAATCAAAGAAATCGGCTGGGCAGATGTCGGAGG
  CTGGACTGGACAAGGAGGGTCCATTCTTGGGACAAAACGCACCCTACCTGGAAAGTACTTGGAGAAGATC
  GCAGAGCAGATGCATTCCCATAGTATCAATGCCCTTCTGATCATTGGAGGATTTGAGGGTTATCATGGTG
  CCATCATGCTATCTGAGGCTCGAAACAACCATGAGGAGTTTTGTATCCCAATATGTGTTTTGCCTGCCAC
  TATTAGCAACGATATCCCTGGCTCAGACATCAGTATCGGTTGTGATACTGCCCTGAATGTGATTGTCGAG
  ACGTGCGACCGCATTAAACAGTCAGCCAGTGGGACCAAGCGCCGGGTGTTCATCATTGAGACCATGGGTG
  GATACTGTGGCTACCTGGCCAACATGGGGGCCCTTGCAGCAGGAGCTGATGCTGCCTACATCTTTGAAGA
  GCCATTTGATATCGGAGATTTGCAGTCCAACGTCGTGCACTTGACAGAGAAAATGAAGACCAGCATCCAG
  AGGGGCCTCGTACTCAGAAACGAAAGCTGCAGTGTAAATTATACCACTGACTTCATCTACCAGCTCTACT
  CAGAGGAGGGCAAAGGAGTGTTTGACTGCAGGAAAAACGTGCTGGGCCATATGCAGCAGGGGGGAGCACC
  GTCTCCATTCGATAGGAACTTTGGAACCAAGATTTCTGCCAAAGCAATGGAGTGGATCTCGGCCAAACTG
  AAGGGGTCCCAGGGCACAGGGAAAAAATTTGTTAGCGATGATTCCATTTGTGTCCTGGGAATTTGCAAGA
  GAGATCTCCTGTTTCAACCAGTGGCAGAGCTGAAGAAAGTCACTGACTTTGAGCACAGGATCCCCAAGGA
  GCAGTGGTGGCTGAAACTGCGGCCCATCATGAAGATCTTGGCCAAGTATGAGGCGAGCTATGACATGTCT
  GATTCAGGCAAGCTGGAGTCCTTGCAGCACCATGAAGAACTATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001291071
• Insert Size: 2355 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001291071.1, NP_001278000.1
• RefSeq Size: 4033 bp
• RefSeq ORF: 2355 bp
• Locus ID: 56421
• UniProt ID: Q9WUA3
• Cytogenetics: 13 A1
• Gene Summary: Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon and contains an alternate in-frame exon, both in the 3' coding region, compared to variant 1. This results in isoform 2, which is of the same size, but includes a different internal in-frame segment, compared to isoform 1.