Postn (NM_001198766) Mouse Untagged Clone

CAT#: MC228927

Postn (untagged) - Mouse periostin, osteoblast specific factor (Postn), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Postn
• Synonyms: A630052E07Rik; AI747096; Os; OSF; OSF-2; Osf2; p; Pe; PLF; PN
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228927 representing NM_001198766
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTCCTCTCCTGCCCTTATATGCTCTGCTGCTGCTGTTCCTGTGTGATATTAACCCTGCAAATGCCA
  ACAGTTACTATGACAAGGTCCTGGCTCACAGCCGCATCAGGGGTCGGGATCAGGGCCCAAACGTCTGTGC
  CCTCCAGCAAATTCTGGGCACCAAAAAGAAATACTTCAGCTCCTGTAAGAACTGGTATCAAGGTGCTATC
  TGCGGGAAGAAAACCACTGTGCTATATGAATGCTGCCCTGGCTATATGAGAATGGAAGGGATGAAAGGCT
  GCCCCGCAGTGATGCCTATTGACCATGTTTATGGCACGCTGGGCATTGTGGGAGCCACTACCACTCAGCA
  CTACTCCGATGTCTCGAAGCTGAGAGAAGAGATTGAAGGAAAAGGGTCATACACGTACTTCGCGCCGAGT
  AACGAGGCTTGGGAGAACCTGGATTCTGACATTCGCAGAGGACTGGAGAACAATGTCAATGTTGAGCTAC
  TGAATGCCTTACACAGCCACATGGTTAATAAGAGAATGTTAACCAAGGACCTGAAACACGGCATGGTTAT
  TCCTTCAATGTACAACAATCTGGGGCTTTTTATTAACCATTATCCCAATGGGGTTGTCACTGTGAACTGT
  GCTCGAGTCATCCATGGGAACCAGATTGCCACAAATGGTGTCGTCCATGTCATTGACCGTGTCCTGACAC
  AAATTGGTACCTCCATCCAAGACTTCCTTGAAGCAGAAGACGACCTTTCATCATTTAGAGCAGCCGCCAT
  CACCTCTGACCTCTTGGAGTCCCTTGGAAGAGATGGTCACTTCACGCTCTTTGCTCCCACCAATGAAGCT
  TTCGAGAAACTGCCACGAGGTGTCCTAGAAAGGATCATGGGAGACAAAGTGGCTTCTGAAGCTCTCATGA
  AGTACCACATCCTAAATACCCTCCAGTGCTCTGAGGCCATCACTGGAGGAGCCGTGTTTGAGACCATGGA
  AGGAAACACTATTGAGATAGGGTGCGAAGGGGACAGTATCTCCATTAACGGAATCAAGATGGTGAACAAG
  AAAGACATTGTGACTAAGAATGGTGTCATCCACCTGATTGATGAAGTCCTCATTCCTGATTCTGCCAAAC
  AAGTTATTGAGCTGGCTGGAAAACAGCAAACCACTTTCACCGACCTGGTAGCCCAATTAGGCTTGGCATC
  CTCTCTGAAGCCAGATGGAGAGTACACCTTATTAGCACCTGTGAACAATGCGTTCTCTGATGACACTCTG
  AGCATGGACCAACGCCTTCTTAAGCTAATTCTGCAAAATCACATATTGAAAGTAAAAGTTGGCCTTAGCG
  ACCTCTACAATGGACAGATACTGGAAACCATTGGAGGCAAACAACTCCGAGTCTTTGTGTATCGGACGGC
  TATCTGCATAGAAAACTCATGCATGGTGAGAGGAAGCAAGCAGGGAAGGAATGGTGCCATTCACATATTC
  CGAGAAATCATCCAACCAGCAGAGAAATCCCTGCACGACAAGCTGCGGCAAGACAAGCGCTTTAGCATCT
  TCCTCAGCCTCCTTGAAGCTGCAGATTTGAAAGATCTCCTGACACAGCCCGGAGATTGGACCTTGTTTGC
  ACCAACCAATGATGCCTTCAAGGGAATGACTAGCGAAGAAAGGGAGCTTCTGATTGGGGATAAAAATGCT
  CTCCAAAACATCATTCTTTATCACCTGACCCCAGGGGTTTATATTGGAAAGGGATTCGAACCCGGAGTCA
  CTAATATCCTGAAGACCACACAGGGAAGCAAAATCTATCTGAAAGGAGTAAACGAAACGCTTCTAGTGAA
  TGAGTTGAAGTCCAAAGAATCTGACATCATGACGACAAATGGTGTCATCCACGTCGTGGACAAACTCCTC
  TATCCAGCAGATATTCCAGTTGGAAATGATCAGCTCTTGGAATTACTGAACAAACTGATAAAATACATCC
  AAATCAAGTTTGTTCGTGGCAGCACCTTCAAAGAAATCCCCATGACTGTCTATAGACCTGCAATGACGAA
  GATCCAAATTGAAGGTGATCCCGACTTCAGGCTGATTAAAGAAGGCGAAACGGTGACAGAAGTGATCCAC
  GGAGAGCCAGTCATTAAAAAGTACACCAAAATCATAGATGGAGTTCCTGTTGAAATAACTGAAAAACAGA
  CTCGGGAAGAACGAATCATTACAGGTCCTGAGATAAAATATACCAGGATTTCCACAGGAGGTGGAGAAAC
  AGGAGAGACCTTGCAGAAATTCTTGCAAAAAGACACACCTGCAAAGAAGATACCAGCCAACAAAAGGGTT
  CAAGGGCCTAGAAGACGATCAAGAGAAGGCCGTTCTCAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001198766
• Insert Size: 2352 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001198766.1, NP_001185695.1
• RefSeq Size: 3130 bp
• RefSeq ORF: 2352 bp
• Locus ID: 50706
• UniProt ID: Q62009
• Cytogenetics: 3 C
• Gene Summary: This gene encodes a secreted extracellular matrix protein that functions in tissue development and regeneration, including wound healing and ventricular remodeling following myocardial infarction. The encoded protein binds to integrins to support adhesion and migration of epithelial cells. This protein plays a role in cancer stem cell maintenance and metastasis. Mice lacking this gene exhibit cardiac valve disease, and skeletal and dental defects. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]
  Transcript Variant: This variant (3) lacks an alternate in-frame exon in the 3' coding region, compared to variant 1. It encodes isoform 3, which is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.