Pear1 (NM_001289601) Mouse Untagged Clone

CAT#: MC228925

Pear1 (untagged) - Mouse platelet endothelial aggregation receptor 1 (Pear1), transcript variant 6

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pear1
• Synonyms: 3110045G13Rik; Jedi-1; Megf12
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228925 representing NM_001289601
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTGTCATTTGTTCCCTGCCATGCCCAGAGGGTTTCCATGGACCCAACTGTACTCAGGAATGTCGCT
  GCCACAACGGTGGCCTCTGTGACAGGTTTACTGGGCAGTGCCACTGTGCTCCTGGCTATATCGGGGATCG
  GTGCCAAGAAGAGTGCCCCGTGGGCCGCTTCGGTCAAGACTGTGCTGAGACCTGTGACTGTGCTCCTGGC
  GCCCGTTGCTTTCCTGCTAATGGCGCGTGTCTGTGTGAACATGGCTTCACAGGCGACCGCTGCACTGAGC
  GCCTCTGTCCGGATGGCCGCTATGGTCTGAGCTGCCAGGAGCCCTGCACCTGCGACCCAGAACACAGTCT
  CAGCTGCCACCCGATGCACGGCGAGTGCTCCTGCCAGCCAGGTTGGGCGGGCCTCCACTGCAACGAGAGC
  TGCCCTCAGGACACGCATGGCCCGGGCTGCCAGGAGCACTGCCTCTGTCTGCACGGAGGGCTCTGCCTTG
  CCGACAGCGGCCTCTGCCGGTGCGCGCCGGGATACACGGGACCTCACTGCGCTAACCTATGTCCACCGGA
  CACTTACGGGATCAACTGTTCCTCCCGCTGCTCCTGTGAAAATGCCATTGCCTGCTCTCCCATCGACGGC
  ACGTGCATCTGCAAGGAAGGTTGGCAGCGTGGTAACTGCTCTGTTCCCTGTCCCCTTGGCACCTGGGGCT
  TCAATTGCAATGCCAGTTGCCAGTGTGCCCACGACGGAGTCTGCAGCCCCCAAACTGGAGCCTGTACTTG
  CACCCCTGGGTGGCATGGTGCTCACTGCCAGCTTCCCTGCCCGAAGGGACAGTTTGGTGAAGGCTGTGCC
  AGTGTCTGTGACTGTGACCACTCTGATGGCTGTGACCCTGTTCATGGACAGTGCCGATGTCAGGCTGGTT
  GGATGGGCACACGCTGCCACCTGCCTTGCCCGGAGGGCTTTTGGGGAGCCAACTGCAGTAACACCTGTAC
  CTGCAAGAATGGTGGTACCTGTGTGTCTGAGAATGGCAACTGCGTGTGCGCACCAGGGTTCCGAGGCCCC
  TCCTGCCAGAGGCCCTGCCCGCCTGGTCGCTATGGCAAACGCTGTGTGCAATGCAAGTGTAACAACAACC
  ATTCTTCCTGCCACCCATCGGACGGGACCTGCTCCTGCCTGGCGGGCTGGACAGGCCCTGACTGCTCCGA
  GGCATGTCCCCCAGGCCACTGGGGACTCAAATGCTCCCAACTCTGCCAGTGTCATCATGGTGGGACCTGC
  CACCCCCAGGATGGGAGCTGTATCTGCACGCCAGGCTGGACTGGACCCAACTGCTTGGAAGGCTGCCCAC
  CAAGAATGTTTGGTGTCAACTGCTCCCAGCTATGTCAGTGTGATCTCGGAGAGATGTGCCACCCAGAGAC
  TGGGGCTTGTGTCTGTCCCCCAGGACACAGTGGTGCAGACTGCAAAATGGGAAGCCAGGAGTCCTTCACC
  ATAATGCCCACCTCTCCCGTGACCCATAACTCACTGGGTGCAGTGATTGGCATTGCAGTACTGGGAACCC
  TCGTGGTGGCCCTGATAGCACTGTTCATTGGCTACCGCCAGTGGCAAAAGGGCAAGGAACATGAGCACTT
  GGCAGTGGCTTACAGCACTGGGCGGCTGGATGGCTCTGATTACGTCATGCCAGATGTCTCTCCGAGCTAT
  AGTCACTACTACTCCAACCCCAGCTACCACACACTGTCTCAGTGTTCTCCTAACCCCCCACCCCCTAACA
  AGGTCCCAGGCAGTCAGCTCTTTGTCAGCTCCCAGGCCCCTGAGCGGCCAAGCAGAGCCCACGGGCGTGA
  GAACCATGTCACACTGCCCGCTGACTGGAAGCACCGCCGGGAGCCCCATGAAAGAGGCGCCAGCCACCTG
  GACCGAAGCTATAGCTGTAGCTATAGCCACAGGAATGGCCCGGGACCATTCTGTCATAAAGGTCCCATCT
  CTGAAGAGGGACTAGGGGCAAGCGTTATGTCCCTGAGCAGTGAGAACCCCTATGCTACCATCCGAGACCT
  GCCCAGCCTGCCTGGGGAACCCCGAGAAAGCGGCTATGTGGAGATGAAAGGACCTCCATCAGTGTCCCCT
  CCCAGGCAGTCTCTTCATCTCCGGGACAGGCAGCAGCGGCAACTGCAGCCACAGAGGGACAGTGGCACCT
  ATGAGCAGCCCAGCCCCTTGAGCCATAATGAAGAGTCTTTGGGCTCCACGCCCCCGCTTCCTCCAGGCCT
  GCCTCCTGGTCACTACGACTCCCCCAAGAACAGCCATATCCCTGGACACTATGACTTGCCTCCAGTACGG
  CATCCTCCATCCCCTCCATCCCGGCGCCAGGACCGCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001289601
• Insert Size: 2349 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289601.1, NP_001276530.1
• RefSeq Size: 4510 bp
• RefSeq ORF: 2349 bp
• Locus ID: 73182
• UniProt ID: Q8VIK5
• Cytogenetics: 3 F1
• Gene Summary: When overexpressed, reduces the number of both early and late non-adherent myeloid progenitor cells.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (6) has several internal differences, which causes translation initiation at an alternate start codon, compared to variant 1. The resulting isoform (c) is shorter at the N-terminus compared to isoform a.