Atp6v0a1 (NM_001243049) Mouse Untagged Clone

CAT#: MC228922

Atp6v0a1 (untagged) - Mouse ATPase, H+ transporting, lysosomal V0 subunit A1 (Atp6v0a1), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Atp6v0a1
• Synonyms: AA959968; ATP6a1; Atp6n1; Atp6n1a; Atpv0a1; Vpp-1; Vpp1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228922 representing NM_001243049
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCGAAAACTCCGATTTGTTGAGAAAGAGATAAGGAAAGCTAACATCCCAATTATGGACACCGGTG
  AAAACCCAGAGGTGCCCTTCCCTCGGGACATGATCGACTTAGAGGCCAATTTCGAGAAGATTGAAAATGA
  ACTGAAGGAAATCAACACTAACCAGGAAGCTCTGAAGAGAAACTTCCTGGAACTGACTGAATTAAAATTT
  ATCCTGCGAAAAACCCAGCAGTTTTTCGATGAGCAGATGGCGGATCCAGACCTGTTGGAAGAGTCCTCAT
  CACTCTTGGAGCCAAACGAGATGGGAAGAGGCGCACCCTTACGACTTGGCTTTGTGGCTGGTGTGATTAA
  CCGGGAGCGGATCCCGACCTTTGAGCGCATGCTTTGGCGAGTGTGCCGAGGGAATGTGTTCCTGAGACAA
  GCTGAAATCGAAAACCCGCTGGAGGATCCTGTGACTGGCGACTACGTGCACAAGTCTGTGTTCATCATTT
  TTTTCCAAGGCGATCAGCTGAAAAACAGGGTCAAGAAAATCTGTGAAGGGTTCCGAGCCTCCCTCTATCC
  CTGCCCTGAGACTCCACAGGAAAGGAAAGAAATGGCTTCCGGAGTCAATACCAGGATCGACGACCTCCAA
  ATGGTTCTGAATCAGACGGAGGACCACCGCCAGAGGGTTCTGCAGGCAGCTGCTAAGAACATCCGCGTCT
  GGTTCATCAAGGTGCGGAAGATGAAGGCTATCTACCATACCCTGAACCTGTGCAATATAGACGTGACCCA
  GAAGTGCCTGATCGCAGAAGTCTGGTGCCCTGTCACTGACCTGGACTCCATCCAGTTTGCACTACGAAGA
  GGCACGGAACACAGTGGGTCCACTGTCCCCTCCATTCTGAACAGGATGCAGACAAACCAGACACCGCCGA
  CATATAATAAAACCAACAAGTTCACGCACGGCTTCCAGAACATAGTAGATGCTTATGGGATCGGCACTTA
  CCGAGAGATTAACCCAGCTCCGTACACTGTCATCACCTTCCCCTTTCTGTTTGCTGTGATGTTCGGAGAT
  TTTGGGCACGGCATTTTGATGACGCTGTTTGCTGTGTGGATGGTGTTGAGGGAGAGCCGGATCCTCTCCC
  AGAAGCACGAGAATGAGATGTTTAGCATGGTGTTCAGCGGCCGGTACATTATTCTTCTGATGGGACTGTT
  CTCCATCTATACTGGACTCATCTACAATGACTGCTTTTCCAAGTCTCTGAATATCTTTGGGTCATCGTGG
  AGTGTACGGCCAATGTTCACTCAAGGGAACTGGACGGAGGAGACGCTTCTGGGGAGTTCTGTTCTCCAGT
  TGAACCCCGCTATCCCTGGAGTTTTTGGTGGCCCGTACCCGTTTGGCATTGATCCGATTTGGAACATTGC
  AACCAACAAGCTGACCTTCCTCAACTCCTTCAAGATGAAGATGTCTGTTATCCTCGGCATCATCCACATG
  CTGTTTGGAGTCAGCCTGAGCCTTTTCAACCATATCTATTTCAAGAAGCCCCTGAACATCTACTTTGGCT
  TTATTCCTGAGATCATCTTCATGTCCTCGTTGTTTGGCTACCTGGTCATCCTTATCTTTTACAAGTGGAC
  AGCCTACGATGCCCACTCGTCTAGGAATGCCCCGAGCCTCCTGATCCACTTCATAAACATGTTCCTCTTC
  TCCTACCCAGAGTCTGGTAATGCAATGCTGTACTCTGGACAGAAAGGAATTCAGTGTTTCCTCATCGTGG
  TGGCGATGCTCTGCGTCCCTTGGATGCTTCTGTTTAAGCCGCTGATCCTTCGCCATCAGTACCTGAGGAA
  GAAGCATTTGGGAACTCTCAACTTTGGTGGGATCAGGGTGGGCAACGGACCGACAGAGGAGGATGCTGAA
  ATTATTCAGCATGACCAGCTTTCCACCCATTCAGAGGACGCAGAAGAGCCTACCGAGGACGAAGTGTTTG
  ACTTTGGCGACACCATGGTCCACCAGGCCATCCACACCATTGAGTACTGCTTGGGTTGTATCTCCAACAC
  CGCGTCGTACCTGCGACTCTGGGCCCTCAGCCTGGCCCACGCACAGCTCTCTGAAGTACTTTGGACCATG
  GTGATCCACATTGGCCTGCACGTCCGGAGTTTGGCCGGGGGTCTAGGGCTGTTTTTCATCTTCGCCGCCT
  TTGCCACGCTGACCGTGGCTATCCTGCTGATCATGGAGGGCCTCTCGGCCTTCCTCCACGCGCTGCGGTT
  ACACTGGGTGGAGTTCCAGAACAAATTCTACACTGGGACTGGCTTCAAGTTCCTCCCCTTCTCCTTTGAG
  CACATTCGGGAAGGGAAGTTTGATGAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001243049
• Insert Size: 2340 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001243049.1, NP_001229978.1
• RefSeq Size: 3841 bp
• RefSeq ORF: 2340 bp
• Locus ID: 11975
• Cytogenetics: 11 64.04 cM
• Gene Summary: Required for assembly and activity of the vacuolar ATPase. Potential role in differential targeting and regulation of the enzyme for a specific organelle (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus than isoform 1.