Tle4 (NM_001302951) Mouse Untagged Clone

CAT#: MC228910

Tle4 (untagged) - Mouse transducin-like enhancer of split 4, homolog of Drosophila E(spl) (Tle4), transcript variant 4

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tle4
• Synonyms: 5730411M05Rik; AA792082; Bce-1; Bce1; ESTM13; ESTM14; grg-4; Grg4; X83332; X83333
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228910 representing NM_001302951
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCGGCTGCCTCCGACGGGCCACTTTCGATTCCGGGCACCGCATCAGCCTGCTCAACCCTTCAAAT
  TTACAATTTCAGAATCCTGTGATCGGATTAAGGAAGAGTTTCAGTTTTTACAGGCTCAATACCACAGTCT
  GAAGCTGGAATGTGAGAAGCTCGCCAGCGAGAAGACAGAGATGCAGCGGCATTATGTCATGTATTATGAA
  ATGTCCTATGGGTTGAACATAGAAATGCACAAGCAGGCAGAGATTGTTAAACGACTAAATGCTATCTGTG
  CACAGGTCATTCCTTTCCTGTCCCAAGAGCACCAGCAACAAGTGGTGCAGGCTGTGGAACGGGCCAAGCA
  GGTGACCATGGCAGAACTGAACGCCATCATTGGGCAGCAACAACTCCAAGCTCAGCATTTATCACATGGA
  CATGGTCTGCCTGTGCCTCTGACACCACACCCTTCAGGGCTTCAGCCCCCAGCCATCCCACCTATCGGTA
  GCAGTGCAGGACTTCTGGCCCTCTCCAGTGCACTAGGAGGTCAGTCTCACCTCCCAATTAAGGATGAGAA
  GAAGCACCATGACAATGATCACCAAAGAGACAGAGACTCCATCAAGAGCTCTTCCGTATCCCCATCAGCC
  AGTTTCCGGGGTTCTGAGAAACATAGGAACTCTACAGACTATTCCTCAGAGAGCAAAAAGCAGAAAACAG
  AAGAAAAAGAAATTGCAGCTCGCTATGACAGTGATGGTGAGAAGAGTGATGACAACTTGGTAGTTGATGT
  GTCCAATGAGGATCCATCTTCCCCCCGAGGAAGCCCAGCACATTCCCCAAGGGAGAATGGCCTGGACAAG
  ACACGACTCCTCAAGAAAGATGCCCCCATCAGCCCCGCTTCTGTTGCATCTTCCAGCAGTACTCCTTCCT
  CCAAATCCAAAGAGCTTAGCCTTAATGAAAAGTCTACTACTCCTGTTTCAAAGTCCAATACCCCTACTCC
  ACGAACTGATGCACCTACCCCTGGCAGTAATTCTACTCCTGGACTGAGGCCTGTACCTGGAAAGCCACCA
  GGTGTGGACCCCTTAGCATCAAGCCTGAGGACCCCGATGGCTGTACCTTGTCCATATCCAACCCCCTTTG
  GGATCGTGCCCCATGCTGGGATGAACGGAGAGCTGACCAGCCCCGGAGCCGCCTATGCTGGGCTACACAA
  CATCTCCCCCCAGATGAGTGCAGCTGCTGCTGCAGCTGCGGCAGCAGCAGCCTATGGAAGATCACCTGTG
  GTGGGATTTGATCCACACCATCACATGCGAGTGCCAGCAATACCTCCAAATCTAACAGGCATTCCAGGAG
  GAAAACCAGCATACTCCTTTCACGTCAGTGCCGATGGCCAGATGCAGCCTGTCCCTTTCCCCCCTGATGC
  CCTCATTGGACCAGGGATCCCCCGACACGCTCGACAGATCAACACCCTCAACCATGGGGAGGTGGTGTGT
  GCAGTGACCATCAGCAACCCCACAAGGCACGTGTACACAGGTGGCAAGGGCTGTGTTAAGGTCTGGGACA
  TCAGCCACCCTGGCAACAAGAGCCCAGTCTCTCAGCTGGACTGTCTGAACAGGGATAACTACATCCGTTC
  CTGCAGATTGCTCCCTGATGGTCGCACCTTAATTGTTGGAGGGGAAGCCAGCACACTGTCCATCTGGGAC
  CTGGCAGCTCCAACTCCACGCATCAAGGCAGAGCTGACATCCTCAGCCCCTGCCTGCTATGCTCTGGCCA
  TCAGCCCCGACTCCAAGGTCTGCTTCTCATGCTGCAGCGACGGTAACATCGCAGTGTGGGATCTGCACAA
  CCAGACTCTGGTGAGGCAATTCCAGGGGCACACAGATGGAGCCAGCTGTATTGACATTTCTAATGATGGC
  ACCAAGCTCTGGACAGGTGGTTTGGACAACACTGTGAGGTCCTGGGACCTGCGTGAAGGGCGGCAGCTGC
  AGCAACATGACTTCACCTCTCAGATCTTTTCATTGGGCTATTGCCCAACTGGAGAGTGGCTTGCAGTGGG
  GATGGAGAATAGCAATGTGGAAGTATTGCATGTCACCAAACCAGACAAATACCAGTTGCATCTTCATGAG
  AGCTGTGTGCTGTCACTCAAGTTTGCCCACTGTGGCAAATGGTTTGTAAGCACTGGAAAGGACAACCTTC
  TGAATGCTTGGAGGACGCCTTATGGGGCCAGCATATTCCAGTCCAAAGAATCCTCATCGGTGCTTAGCTG
  TGACATCTCTGTGGATGACAAGTACATTGTCACTGGCTCTGGGGACAAGAAAGCTACGGTTTATGAAGTT
  ATTTATTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001302951
• Insert Size: 2319 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001302951.1, NP_001289880.1
• RefSeq Size: 4067 bp
• RefSeq ORF: 2319 bp
• Locus ID: 21888
• Cytogenetics: 19 9.11 cM
• Gene Summary: Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by PAX5, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Essential for the transcriptional repressor activity of SIX3 during retina and lens development and for SIX3 transcriptional auto-repression.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (4) uses an alternate 5'-terminal exon, which results in a distinct 5' UTR and use of an alternate start codon, compared to variant 1. The encoded protein (isoform 4) is shorter and has a distinct N-terminus compared to isoform 1.