App (NM_001198823) Mouse Untagged Clone

CAT#: MC228903

App (untagged) - Mouse amyloid beta (A4) precursor protein (App), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: App
• Synonyms: Abeta; Abpp; Adap; Ag; betaApp; Cvap; E030013M08Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228903 representing NM_001198823
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCCCAGCTTGGCACTGCTCCTGCTGGCCGCCTGGACGGTTCGGGCTCTGGAGGTACCCACTGATG
  GCAACGCCGGGCTGCTGGCAGAACCCCAGATCGCCATGTTCTGTGGTAAACTCAACATGCACATGAATGT
  GCAGAATGGAAAGTGGGAGTCAGACCCGTCAGGGACCAAAACCTGCATTGGCACCAAGGAGGGCATCTTG
  CAGTACTGCCAAGAGGTCTACCCTGAACTGCAGATCACAAACGTGGTGGAAGCCAACCAGCCAGTGACCA
  TCCAGAACTGGTGCAAGCGGGGCCGCAAGCAGTGCAAGACACACACCCACATCGTGATTCCTTACCGTTG
  CCTAGTTGGTGAGTTTGTGAGCGACGCCCTTCTCGTGCCCGACAAGTGCAAGTTCCTACACCAGGAGCGG
  ATGGATGTTTGTGAGACCCATCTTCACTGGCACACCGTCGCCAAAGAGACATGCAGCGAGAAGAGCACTA
  ACTTGCACGACTATGGCATGCTGCTGCCCTGCGGCATCGACAAGTTCCGAGGGGTAGAGTTTGTATGCTG
  CCCGTTGGCCGAGGAAAGCGACAGCGTGGATTCTGCGGATGCAGAGGAGGATGACTCTGATGTCTGGTGG
  GGTGGAGCGGACACAGACTACGCTGATGGCGGTGAAGACAAAGTAGTAGAAGTCGCCGAAGAGGAGGAAG
  TGGCTGATGTTGAGGAAGAGGAAGCTGATGATGATGAGGATGTGGAGGATGGGGACGAGGTGGAGGAGGA
  GGCCGAGGAGCCCTACGAAGAGGCCACCGAGAGAACAACCAGCACTGCCACCACCACCACAACCACCACT
  GAGTCCGTGGAGGAGGTGGTCCGAGAGGTGTGCTCTGAACAAGCCGAGACCGGGCCATGCCGCGCAATGA
  TCTCCCGCTGGTACTTTGATGTCACTGAAGGGAAGTGTGTCCCATTCTTTTACGGCGGATGTGGCGGCAA
  CAGGAACAACTTTGACACGGAAGAGTACTGCATGGCGGTGTGTGGCAGCGTGTCAACCCAAAGTTTACTC
  AAGACTACCAGTGAACCTCTTCCCCAAGATCCTGATAAACTTCCCACGACAGCAGCCAGCACCCCCGACG
  CCGTCGACAAGTACCTGGAGACACCCGGGGACGAGAACGAGCATGCCCATTTCCAGAAAGCCAAAGAGAG
  GCTGGAAGCCAAGCACCGAGAGAGAATGTCCCAGGTCATGAGAGAATGGGAAGAGGCAGAGCGTCAAGCC
  AAGAACTTGCCCAAAGCTGACAAGAAGGCCGTTATCCAGCATTTCCAGGAGAAAGTGGAATCTCTGGAAC
  AGGAAGCAGCCAATGAGAGACAGCAGCTTGTAGAGACACACATGGCCAGAGTTGAAGCCATGCTCAATGA
  CCGCCGCCGCCTGGCCCTCGAGAATTACATCACTGCACTGCAGGCGGTGCCCCCAAGGCCTCATCATGTG
  TTCAACATGCTGAAGAAGTACGTCCGTGCGGAGCAGAAAGACAGACAGCACACCCTAAAGCATTTTGAAC
  ATGTGCGCATGGTGGACCCCAAGAAAGCTGCTCAGATCCGGTCCCAGGTTATGACACACCTCCGTGTGAT
  CTACGAGCGCATGAACCAGTCTCTGTCCCTGCTCTACAATGTCCCTGCGGTGGCTGAGGAGATTCAAGAT
  GAAGTCGATGAGCTGCTTCAGAAGGAGCAGAACTACTCCGACGATGTCTTGGCCAACATGATCAGTGAGC
  CCAGAATCAGCTACGGAAACGACGCTCTCATGCCTTCGCTGACGGAAACCAAGACCACCGTGGAGCTCCT
  TCCCGTGAATGGGGAATTCAGCCTGGATGACCTCCAGCCGTGGCACCCTTTTGGGGTGGACTCTGTGCCA
  GCCAATACCGAAAATGAAGTCGAGCCTGTTGACGCCCGCCCCGCTGCTGACCGAGGACTGACCACTCGAC
  CAGGTTCTGGGCTGACAAACATCAAGACGGAAGAGATCTCGGAAGTGAAGATGGATGCAGAATTCGGACA
  TGATTCAGGATTTGAAGTCCGCCATCAAAAACTGGTGTTCTTTGCTGAAGATGTGGGTTCGAACAAAGGC
  GCCATCATCGGACTCATGGTGGGCGGCGTTGTCATAGCAACCGTGATTGTCATCACCCTGGTGATGTTGA
  AGAAGAAACAGTACACATCCATCCATCATGGCGTGGTGGAGGTCGACGCCGCCGTGACCCCAGAGGAGCG
  CCATCTCTCCAAGATGCAGCAGAACGGATATGAGAATCCAACTTACAAGTTCTTTGAGCAAATGCAGAAC
  TAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001198823
• Insert Size: 2313 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001198823.1, NP_001185752.1
• RefSeq Size: 3377 bp
• RefSeq ORF: 2313 bp
• Locus ID: 11820
• UniProt ID: P12023
• Cytogenetics: 16 46.92 cM
• Gene Summary: Functions as a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. Interaction between APP molecules on neighboring cells promotes synaptogenesis. Involved in cell mobility and transcription regulation through protein-protein interactions. Can promote transcription activation through binding to APBB1-KAT5 and inhibit Notch signaling through interaction with Numb. Couples to apoptosis-inducing pathways such as those mediated by G(O) and JIP. Inhibits G(o) alpha ATPase activity (By similarity). Acts as a kinesin I membrane receptor, mediating the axonal transport of beta-secretase and presenilin 1. May be involved in copper homeostasis/oxidative stress through copper ion reduction. Can regulate neurite outgrowth through binding to components of the extracellular matrix such as heparin and collagen I and IV (By similarity). The splice isoforms that contain the BPTI domain possess protease inhibitor activity. Induces a AGER-dependent pathway that involves activation of p38 MAPK, resulting in internalization of amyloid-beta peptide and leading to mitochondrial dysfunction in cultured cortical neurons (By similarity). Provides Cu(2+) ions for GPC1 which are required for release of nitric oxide (NO) and subsequent degradation of the heparan sulfate chains on GPC1.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).