Fgd4 (NM_001301818) Mouse Untagged Clone

CAT#: MC228886

Fgd4 (untagged) - Mouse FYVE, RhoGEF and PH domain containing 4 (Fgd4), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Fgd4
• Synonyms: 9030023J02Rik; 9330209B17Rik; Fr; Frabp; ZFYVE; ZFYVE6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228886 representing NM_001301818
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGAGTCTAATCCGGCCCCTACTTCTTGTACCTCAAAAGGGAAGCATAGTAAGGTATCGGATCTCA
  TCAGCCACTTTGAAGGAGGCAGTGTTTTGTCAAGTTACATTGATTTGCAGAAAGATTCTACTATGAACCT
  CAATATTCCTCAAACTCTAGGACAGCCTGGGTTAACCTCCTCACCTCCACGAAAATTTCTGCCCCAGCAC
  TCTCCACAGAAGCAGGAAAACGACCCAGATCAGACTCAGGGGCAACATGGTTGTTTGGCCAATGGTGTAG
  TGGCTGCACAAAACCAGATGGAATGTGAAGATGAGAAGGAGACTACTCTTAGCCCAGAGATGGCTATTCA
  GACTGCTGCCGCCTCTCCTGATACACATGTGCTGAATGGAGAAAGAAATGAAACCATCACAGATTCTGCA
  TCATCCATAGCCAATAGTCATGATGAAAATGCTTCTGACAGCAGCTGCAGGACTCCAGGGACGGACTTAG
  GGCTTCCCTCAAAAGAAGGGGAGCCAGGGATGGACGCTGAGCTCCAAGAGAGGGAAAATGGGGTGAACAC
  CATGGGATTGGATACGTTGGACCAGCACCATGAAGTGAAGGAGACTAATGAACAGAAACTTCACAAAATA
  GCCACTGAACTTTTACTTACAGAAAGAGCTTATGTCAGCCGGCTCGACCTCCTAGATCAGGTATTTTATT
  GCAAACTATTAGAAGAAGCAAACCGAGGCTCATTTCCTGCAGAGATGGTGAATAAAATCTTTTCTAACAT
  TTCATCAATAAATGCCTTCCATAGTAAATTCCTATTACCTGAGCTGGAGAAACGAATGCAAGAATGGGAA
  ACTACACCCAGAATTGGAGATATCCTGCAAAAGTTGGCGCCATTCCTTAAGATGTATGGAGAATACGTGA
  AGGGATTTGATAATGCAGTGGAACTGGTTAAAACCATGACAGAGCGTGTTCCCCAGTTTAAATCAGTGAC
  TGAAGAGATTCAGAAACAGAAGATCTGTGGAAGCTTAACGCTGCAGCATCACATGCTGGAGCCTATTCAG
  CGCATTCCTCGCTATGAGATGCTCCTGAAGGACTACCTGAAGAAGTTGTCTCCTGACTCCCCAGACTGGA
  ATGATGCAAAAAAGTCACTTGAAATTATATCTACAGCAGCAAGCCATTCTAATAGTGCAATAAGAAAAAT
  GGAGAACCTGAAGAAACTTTTAGAAATTTATGAGATGTTGGGAGAAGAAGAGGATATTGTAAATCCCTCA
  AATGAACTAATAAAAGAAGGACAAATCCTCAAACTAGCAGCTCGGAACACATCAGCACAAGAGCGCTACC
  TCTTCTTATTCAACAACATGTTGCTATATTGTGTGCCCAGATTCAGCTTGGTTGGCTCAAAATTCACAGT
  TCGAACCAGAGTTGGCATTGATGGAATGAAAATTGTGGAGACTCACAATGAAGAATATCCACACACTTTC
  CAGATATCTGGGAAAGAAAGAACCCTGGAGCTGCAGGCCAGTTCTGAACAAGACAAGGAAGAATGGATCA
  AGGCCCTTCAAGAAAGTATTGATGCTTTTCATCAAAGGCATGAAACTTTCAGAAATGCAATCGCAAAGGA
  AAATGACATTCCCCTAGAAGTTTCTACTGCTGAGCTGGGAAAACGAGCTCCAAGATGGATACGTGATAAT
  GAAGTGACCATGTGTATGAAGTGCAAAGAGTCCTTCAATGCACTGACCAGAAGGCGGCATCACTGCCGGG
  CATGTGGACATGTGGTTTGTTGGAAATGTTCTGACTACAAAGCTCAGCTTGAGTATGATGGTGGAAGATT
  GAACAAGGTTTGTAAAGACTGTTACCAGATAATAAGTGGATTCACAGACAGTGAAGAAAAGAAAAGAAGG
  GGCATTTTAGAGATTGAATCAGCAGAAGTATCAGGAAATAGTGAGGTGTGCAGTTTCCTTCAGTACATGG
  AAAAGTCAAAGCCTTGGCAGAAAATCTGGTGTGTGATCCCCAAGCAAGACCCCCTTGTGCTGTACATGTA
  TGGCGCTCCTCAGGATGTCAGAGCCCAAGCGACCATACCCCTCCTGGGCTACGTTGTGGATGATATGCCT
  AAGAGTGCAGATCTGCCACATAGTTTCAAGCTGACCCAGTCCAAGTCTGTGCATAGCTTTGCTGCAGACA
  ATGAGGAACTGAAACAGAAATGGCTGAAAATCATCCTTCTAGCTGTCACAGGTGAGACACCAGATGGCCC
  AAGTGAGCATCTAGCCACCTTGAACAATCTCCCTGGACCCAAGAAAAAGTCAGAATGCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001301818
• Insert Size: 2301 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001301818.1, NP_001288747.1
• RefSeq Size: 8324 bp
• RefSeq ORF: 2301 bp
• Locus ID: 224014
• UniProt ID: Q91ZT5
• Cytogenetics: 16 A2- A3
• Gene Summary: This gene is a member of the FYVE, RhoGEF and PH domain containing (FGD) family. The encoded protein is a Cdc42-specific guanine nucleotide exchange factor (GEF) that plays an essential role in regulating the actin cytoskeleton and cell morphology. Disruption of the gene in mouse causes abnormal nerve development and dysmyelination. Mutations in a similar gene in human can cause Charcot-Marie-Tooth disease type 4H (CMT4H), a disorder of the peripheral nervous system. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2014]
  Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream in-frame start codon, compared to variant 1. It represents the use of an alternate promoter. The resulting isoform (alpha) has a shorter N-terminus, compared to isoform 1. Variants alpha and 2 encode the same isoform (alpha).