Kcnc3 (NM_001290682) Mouse Untagged Clone

CAT#: MC228877

Kcnc3 (untagged) - Mouse potassium voltage gated channel, Shaw-related subfamily, member 3 (Kcnc3), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Kcnc3
• Synonyms: Kcr2-3; KShIIID; Kv3.3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228877 representing NM_001290682
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCAGTTCAGTGTGCGTCTGGTCGTTCCGCGGGCGCCAGGGGACCGGCAAGCAGCAGCCTCAGCCGG
  TGCCAACGCCGCAGCCGCCTGAGTCCTCACCGCCGCCTCTGCCGCCGCCGCAGCAGCAGCAGTGCTCTCA
  GCCCGGCACTGCCGCCTCCCCGGCGGGTGCCCCGCTTTCCTGCGGGCCTGGGGGCCGGCGTGCCGAGCCA
  TGCCCCGGGCTGCCGGCGGTGGCCATGGGGCGGCACGGCGGCGGCGGCGGCGACAGCGGTAAGATCGTGA
  TCAACGTGGGCGGCGTGCGCCATGAGACGTACCGCTCCACGTTGCGCACCCTGCCAGGGACCAGACTGGC
  CGGGCTGACCGAGCCCGAGGCGGCCGCGCGCTTTGACTACGACCCGGGCACGGACGAGTTCTTCTTCGAC
  CGTCACCCGGGCGTCTTCGCCTACGTGCTCAACTACTACCGCACCGGCAAACTGCACTGCCCGGCCGACG
  TGTGCGGGCCGCTCTTCGAGGAGGAGCTGGGCTTCTGGGGCATAGACGAGACGGACGTGGAGGCCTGCTG
  CTGGATGACCTATCGCCAGCACCGTGACGCTGAGGAGGCACTGGACTCTTTCGAGGCCCCGGACTCCTCG
  GCCAACGCCAACGCCAACGCCGGAGGCGCGCACGATGCGGGACTGGACGACGAGGCGGGCGCAGGAGGCG
  GCGGCCTGGACGGGGCAGGCGGCGAGCTCAAGCGTCTGTGTTTTCAGGACGCGGGCGGAGGTGCCGGAGG
  ACCTGCCGGGGGCGCGGGCGGCGCGGGCGGCACCTGGTGGCGGCGCTGGCAGCCCCGTGTGTGGGCGCTT
  TTTGAGGACCCCTACTCGTCGCGGGCTGCCAGGTATGTGGCCTTCGCCTCCCTATTCTTTATCCTCATCT
  CCATCACCACCTTCTGCCTGGAGACACACGAGGGCTTCATCCACATCAGCAACAAGACGGTGACGCAGGC
  CTCCCCAATCCCTGGGGCTCCCCCGGAGAATATCACCAACGTGGAGGTGGAGACGGAACCCTTCTTGACC
  TACGTGGAAGGCGTGTGTGTGGTCTGGTTCACCTTTGAGTTTCTCATGCGGGTCACCTTCTGCCCAGATA
  AGGTGGAGTTTCTCAAAAGCAGCCTGAACATCATCGACTGCGTGGCCATCTTGCCCTTCTACTTGGAAGT
  GGGCCTGTCAGGTCTCAGCTCCAAAGCTGCCAAGGACGTGCTGGGCTTCCTGCGTGTCGTCCGCTTCGTG
  CGCATCCTTCGTATCTTCAAGCTGACCCGTCACTTCGTGGGGCTGCGTGTGCTGGGCCACACGCTCCGGG
  CCAGCACCAACGAATTCTTGTTACTCATCATCTTCCTGGCTCTGGGGGTCCTCATCTTTGCCACCATGAT
  CTACTATGCCGAACGCATCGGGGCTGATCCTGACGACATCCTGGGCTCCAACCACACCTACTTCAAGAAC
  ATCCCCATCGGCTTCTGGTGGGCTGTGGTCACCATGACGACACTGGGCTATGGAGACATGTATCCCAAGA
  CGTGGTCTGGGATGCTGGTTGGGGCACTGTGTGCCCTGGCTGGTGTGCTGACCATTGCCATGCCCGTGCC
  TGTCATTGTCAACAACTTCGGCATGTACTATTCACTGGCTATGGCCAAGCAGAAATTGCCAAAGAAGAAA
  AACAAACATATTCCCAGGCCCCCGCAGCCTGGCTCACCCAACTACTGCAAGCCTGACCCCCCGCCTCCAC
  CCCCACCACACCCCCACCACGGCAGCGGTGGCATAAGCCCACCGCCGCCCATCACCCCTCCTTCCATGGG
  GGTGAATGTGGCCGGGGCCTACCCACCTGGACCCCACACACACCCCGGGCTGCTCAGGGGTGGTGCTGGG
  GGCCTGGGAATCATGGGATTGCCTCCTCTGCCAGCCCCTGGTGAGCCCTGCCCATTGGCTCAAGAAGAGG
  TGATTGAAACCAACAGGGCAGACCCCCGTCCCAATGGAGACCCTGCAGCAGCCGCACTGGCCCATGAGGA
  CTGCCCTGCCATCGACCAGCCAGCCATGTCTCCAGAAGACAAGAGCCCAATCACTCCCGGAAGCCGGGGT
  CGCTACAGCCGGGACCGAGCTTGCTTCCTTGTCACAGACTATGCCCCTTCCCCTGATGGCTCCATCCGAA
  AAGCCACTGGTGCTCCCCCACTGCCCCCCCCATGCTGGCATAAGCCAGGCCCCCCAAGCTTCTTGCCCGA
  CCTCAACGCCAACGCAGCAGCCTGGATATCCCCCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001290682
• Insert Size: 2277 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001290682.1, NP_001277611.1
• RefSeq Size: 5184 bp
• RefSeq ORF: 2277 bp
• Locus ID: 16504
• Cytogenetics: 7 28.85 cM
• Gene Summary: Voltage-gated potassium channel that plays an important role in the rapid repolarization of fast-firing brain neurons. The channel opens in response to the voltage difference across the membrane, forming a potassium-selective channel through which potassium ions pass in accordance with their electrochemical gradient. The channel displays rapid activation and inactivation kinetics (PubMed:18539595, PubMed:26997484, PubMed:24218544). It plays a role in the regulation of the frequency, shape and duration of action potentials in Purkinje cells (PubMed:15217387, PubMed:18448641, PubMed:24218544). Required for normal survival of cerebellar neurons, probably via its role in regulating the duration and frequency of action potentials that in turn regulate the activity of voltage-gated Ca(2+) channels and cellular Ca(2+) homeostasis (PubMed:24218544). Required for normal motor function (PubMed:16923152, PubMed:18448641). Plays a role in the reorganization of the cortical actin cytoskeleton and the formation of actin veil structures in neuronal growth cones via its interaction with HAX1 and the Arp2/3 complex (PubMed:26997484).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) contains an alternate exon compared to variant 1. The resulting isoform (2) has a shorter and distinct C-terminus compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.