Mfn2 (NM_001285921) Mouse Untagged Clone

CAT#: MC228872

Mfn2 (untagged) - Mouse mitofusin 2 (Mfn2), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mfn2
• Synonyms: D630023P19Rik; Fzo
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228872 representing NM_001285921
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCCTGCTCTTTTCTCGATGCAACTCCATCGTCACCGTCAAGAAGGATAAGCGACACATGGCTGAAG
  TGAATGCTTCCCCTCTCAAGCACTTTGTCACTGCCAAGAAAAAGATCAATGGAATCTTTGAGCAGCTGGG
  GGCCTACATCCAAGAGAGCGCCAGCTTCCTTGAAGACACCCACAGGAACACAGAACTGGACCCGGTTACC
  ACGGAAGAGCAGGTCCTGGACGTCAAAGGGTACCTGTCCAAGGTCAGGGGTATCAGCGAAGTGCTGGCCA
  GGCGGCACATGAAGGTGGCTTTTTTTGGCCGGACGAGCAATGGGAAGAGCACCGTGATCAATGCCATGCT
  CTGGGACAAAGTTCTGCCATCTGGGATTGGTCATACCACCAATTGCTTCCTGCGGGTTGGGGGCACAGAT
  GGCCATGAGGCCTTCCTCCTCACAGAGGGCTCAGAAGAGAAGAAGAGTGTCAAGACTGTGAACCAACTGG
  CCCATGCCCTCCATCAGGACGAGCAGTTGCATGCAGGCAGCATGGTGAGTGTGATGTGGCCCAACTCCAA
  GTGTCCGCTCCTGAAGGATGACCTCGTGCTGATGGACAGCCCTGGGATCGATGTTACCACGGAGCTGGAC
  AGCTGGATTGATAAGTTTTGCCTGGATGCTGATGTGTTTGTGCTGGTGGCCAACTCAGAGTCCACGCTGA
  TGCAGACGGAGAAGCAGTTCTTCCACAAAGTGAGTGAACGTCTCTCCCGGCCCAACATCTTCATCCTGAA
  CAACCGCTGGGATGCGTCTGCCTCGGAGCCTGAGTACATGGAGGAGGTGCGGCGGCAGCACATGGAGCGC
  TGCACCAGCTTTCTGGTGGATGAGCTGGGCGTGGTGGATCGAGCTCAGGCTGGGGACCGGATCTTCTTCG
  TGTCTGCCAAGGAGGTTCTCAGCGCCAGGGTCCAGAAAGCCCAGGGCATGCCAGAAGGAGGCGGCGCTCT
  CGCAGAAGGTTTTCAAGTGAGGATGTTTGAGTTTCAGAATTTCGAGAGGCAGTTTGAGGAGTGCATTTCC
  CAGTCTGCAGTAAAGACCAAATTTGAGCAGCACACAGTCCGGGCCAAGCAGATTGCAGAGGCCGTTCGTC
  TCATCATGGATTCCCTGCACATCGCAGCTCAGGAGCAGCGGGTTTATTGCCTAGAAATGCGGGAAGAGCG
  GCAAGACCGGCTGAGGTTTATTGACAAGCAGCTGGAGCTCCTGGCTCAAGACTACAAGCTGCGAATTAAG
  CAGATTACGGAGGAAGTGGAAAGGCAGGTGTCCACAGCCATGGCTGAAGAGATCAGGCGCCTCTCTGTGC
  TAGTTGACGAGTACCAGATGGACTTCCACCCATCCCCAGTTGTCCTCAAGGTTTATAAGAACGAGCTGCA
  CCGCCATATAGAGGAAGGTCTGGGCCGGAACCTGTCTGACCGCTGCTCCACTGCCATTGCCAGTTCACTG
  CAGACTATGCAGCAGGACATGATAGACGGCTTGAAGCCCCTTCTTCCTGTATCTATGCGGAATCAGATAG
  ACATGCTGGTCCCTCGACAGTGTTTCTCCCTCAGCTATGACCTGAATTGTGACAAGCTGTGTGCTGACTT
  TCAGGAGGACATCGAGTTCCACTTCTCCCTTGGATGGACTATGCTAGTGAACAGGTTCCTGGGCCCCAAG
  AATAGCCGCCGGGCCTTGCTAGGCTACAGTGATCAGGTTCAGCGTCCTCTCCCTCTGACACCTGCCAACC
  CCAGCATGCCCCCCTTGCCACAGAGCTCCCTCACCCAGGAGGAGCTCATGGTCTCCATGGTTACTGGCCT
  GGCCTCTCTGACGTCTAGGACCTCCATGGGCATTCTTGTGGTCGGAGGAGTGGTGTGGAAGGCAGTGGGC
  TGGAGACTCATCGCCCTCTCCTTTGGACTGTATGGCCTCCTGTACGTCTATGAGCGACTGACCTGGACCA
  CCAAAGCCAAAGAGAGGGCCTTCAAGCGCCAGTTTGTGGAATACGCCAGTGAGAAGCTACAGCTCATCAT
  CAGTTACACCGGCTCTAACTGCAGCCACCAAGTCCAGCAGGAATTGTCTGGGACATTTGCTCATCTGTGC
  CAGCAAGTTGACATCACCCGAGATAATCTGGAGCAGGAAATTGCTGCCATGAACAAGAAAGTCGAGGCTC
  TGGATTCACTTCAGAGCAGAGCCAAACTGCTCAGGAATAAAGCTGGCTGGTTGGACAGCGAACTCAACAT
  GTTCACACACCAGTACCTGCAGCCCAGCAGATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001285921
• Insert Size: 2274 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001285921.1, NP_001272850.1
• RefSeq Size: 4542 bp
• RefSeq ORF: 2274 bp
• Locus ID: 170731
• UniProt ID: Q80U63
• Cytogenetics: 4 E1
• Gene Summary: Mitochondrial outer membrane GTPase that mediates mitochondrial clustering and fusion (PubMed:12527753, PubMed:23921378, PubMed:23620051). Mitochondria are highly dynamic organelles, and their morphology is determined by the equilibrium between mitochondrial fusion and fission events. Overexpression induces the formation of mitochondrial networks. Membrane clustering requires GTPase activity and may involve a major rearrangement of the coiled coil domains (By similarity). Plays a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes. Plays an important role in the regulation of vascular smooth muscle cell proliferation (By similarity). Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). Is required for PRKN recruitment to dysfunctional mitochondria (PubMed:23620051). Involved in the control of unfolded protein response (UPR) upon ER stress including activation of apoptosis and autophagy during ER stress (PubMed:23921556). Acts as an upstream regulator of EIF2AK3 and suppresses EIF2AK3 activation under basal conditions (PubMed:23921556).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. All variants (1 through 7) encode the same protein.