Gfm2 (NM_001271464) Mouse Untagged Clone

CAT#: MC228861

Gfm2 (untagged) - Mouse G elongation factor, mitochondrial 2 (Gfm2), transcript variant 4

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gfm2
• Synonyms: 6530419G12Rik; A930009M04Rik; EF-G2mt; EFG2; MST027; RRF2mt
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228861 representing NM_001271464
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCACCAAATGGAGGATTTTTGCAGTGAATCATCAGAGAACGTTCAGTGTGCACCTTAATACCATGT
  GTTACTGCAAAATAAAAGCAAATTTAAAAAGATTAAAGACTCAGCTGCCTCTTACAAGAAACTACAGTTC
  TGCACCAGGCATAGCAGGAAGTGATGTCAAGTCTCTTCATTCCGTCATTAACCCTCCTGTGGCCAAAATT
  CGTAATATTGGAATTATGGCACATATTGATGCAGGCAAAACCACCACCACAGAGAGAATCCTGTACTATT
  CTGGATACACAAGATCATTGGGAGATGTCGATGATGGAGACACAGTGACGGATTTCATGGCTCAAGAGCG
  AGAAAGGGGCATTACCATCCAGTCAGCAGCTGTGACTCTCGACTGGAAAGGGTATAGAGTCAATCTGATT
  GATACTCCAGGTCACGTAGACTTCACTCTGGAGGTCGAGCGCTGCTTGAGGGTGTTGGACGGTGCGGTGG
  CCGTGTTTGACGCCTCTGCTGGTGTAGAGGCCCAGACTCTCACTGTATGGAGGCAAGCTGACAAACACAA
  AATACCCCGGATCTGCTTTTTAAACAAGATGGACAAGACTGGAGCAAGTTTTAACTATGCGGTTGAAAGC
  ATCCGGGAGAAGTTAAAGGCAAAGCCCCTGATTTTACAGCTGCCAATTGGTGAAGCCAGAACTTTCCAAG
  GAGTGGTGGATGTCGTAAACAAAGAAAAACTCCTTTGGAATTCTAACTCTGACGATGGAAAAGACTTCGA
  GAGGATGCCCCTCTCAGAAGCGAGTGATCGGGAACTGCTAAAGGAGACAATTGAAGCAAGGAACTCCTTA
  ATTGAACAATTACAGGCTGCAGTACACAGGGTGACACTGGCACAGGCTGCAGTGCCTGTGCTGTGTGGGA
  GTGCCTTGAAGAACAAGGGCGTGCAGCCCCTGCTGGATGCGGTGACCACTTACCTGCCGTCACCCGAGGA
  GCGCGAGGACCGCTTTCTGCAGTGGTATGAGGGTGACTTGTGTGCACTAGCGTTTAAAGTACTACATGAC
  AAACAGCGAGGACCCCTGGTTTTCCTGCGCATTTACTCGGGAACACTGACGCCTCAGCTGGCTGTCCACA
  ACATTAATAGAAACTGCACGGAAAGAATGAGTCGTCTGCTTTTGCCATTTGCTGACCAACATGTAGAAAT
  CCCTTCCCTGACTGCTGGAAACATTGCTTTGACAGTTGGGCTTAAACAAACTGCCACTGGAGACACCATA
  GTCTCGTCCAAGTCCAGCGCATTAGCTGCAGCTCGCAGAGCCGGACGAGGAGAAAGGGAGCATGGAAAGA
  AGAGAGAGGCCGAGAGTCTGCTGTTGGCCGGGGTGGAGGTCCCAGAGCCTGTCTTCTTCTGCACCATAGA
  GCCCCCGTCAGTCGCTAAGCAGCCAGATTTAGATCATGCCTTGGAACGCCTTCAGCGCGAAGATCCCAGT
  TTGAAAGTGAAACTCGATCCTGACTCTGGACAAACTGTCCTGTGTGGAATGGGGGAGTTGCATATTGAGA
  TTATTCATGACCGTATCAAGAGGGAGTATGGCCTAGAGACCTATCTAGGGCCTCTGCAGGTGGCATACCG
  GGAAACCATCTTAAACTCAGTTCGCGCCACAGACACCCTCGATAGGGTGCTTGGAGACAAGCGGCACTTG
  GTGAGTGCGGAGCTGGAAGTCCGGCCAGCAGAAGAGCCCTGTGCTGTGGCCAAGATCGAGTATGCTGACT
  GTGTGGGTGAAGACCTGCTGCAGGCCTCTCGGGAGGCCATTGAAAGTGCCGTCCACAGTGCATGCCTCCA
  GGGACCTCTGCTCGGATCCCCAGTCCAGGATGTGGCAATGACGTTGCACTCACTGATGATTCACCCTGGC
  ACCTCCACAACTATGGTCACTGCCTGCATCTCACGGTGTATGCAGAAGGCCCTGAAGAAAGCTGACAAAC
  AGGTGCTGGAACCTCTGATGAGTCTCGAGGTCACAGTGAGCAGGGAGTACCTCAGCCCTGTCCTGGCAGA
  CTTGGCACAAAGAAGAGGGAACATTCAGGAAATCCAGACTCGCCAAGACAACAGAGTTGTTCTAGGATTC
  GTTCCTCTAGCAGAGATTATGGGATATTCAACTGTGCTTCGAACTCTGACATCTGGCTCAGCTACTTTTG
  CCTTAGAACTGTCTACTTATCAAGCCATGAGTCCTCAAGACCAGAGTGCTCTACTCAACCAGAGAAGTGG
  CTTGGCCCATGTGCTATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001271464
• Insert Size: 2259 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001271464.1, NP_001258393.1
• RefSeq Size: 3084 bp
• RefSeq ORF: 2259 bp
• Locus ID: 320806
• UniProt ID: Q8R2Q4
• Cytogenetics: 13 D1
• Gene Summary: Mitochondrial GTPase that mediates the disassembly of ribosomes from messenger RNA at the termination of mitochondrial protein biosynthesis. Acts in collaboration with MRRF. GTP hydrolysis follows the ribosome disassembly and probably occurs on the ribosome large subunit. Not involved in the GTP-dependent ribosomal translocation step during translation elongation.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (4) uses an alternate splice site in the 5' UTR and lacks an in-frame exon, compared to variant 1. The encoded isoform (3) is shorter than isoform 1.