Pla2g6 (NM_001199025) Mouse Untagged Clone

CAT#: MC228858

Pla2g6 (untagged) - Mouse phospholipase A2, group VI (Pla2g6), transcript variant 4

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pla2g6
• Synonyms: BB112799; iPLA(2)beta; iPLA2; iPLA2beta; PNPLA9
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228858 representing NM_001199025
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGTTCTTCGGACGCCTCGTCAACACCCTCAGTAGCGTCACCAACTTGTTCTCGAACCCATTCCGGG
  TGAAGGAGGTGTCCCTGACTGACTACGTCTCAAGTGAACGTGTCCGGGAGGAAGGGCAGCTGATCCTGTT
  ACAGAATGTCTCCAATCGCACCTGGGACTGTGTCCTGGTCAGCCCGAGGAACCCACAGAGCGGCTTCCGG
  CTCTTCCAACTGGAGTCTGAGGCAGACGCCCTGGTGAACTTCCAGCAGTTCTCCTCCCAGCTGCCGCCCT
  TCTACGAGAGCTCTGTGCAGGTCCTGCATGTGGAGGTGCTTCAGCACCTGACCGACCTCATCCGGAACCA
  CCCCAGCTGGACAGTGACACACCTAGCCGTGGAGCTCGGCATCCGGGAGTGCTTCCATCACAGCCGCATC
  ATCAGCTGTGCCAACAGCACAGAGAATGAGGAGGGCTGCACCCCACTACATCTGGCCTGCCGCAAGGGTG
  ACAGTGAGATCCTGGTGGAGCTGGTACAATACTGCCACGCCCAGATGGATGTCACTGACAACAAAGGAGA
  GACTGCCTTCCATTACGCTGTGCAAGGGGACAATCCCCAGGTGCTACAGCTCCTAGGGAAGAACGCCTCA
  GCCGGCCTGAACCAGGTAAACAACCAAGGGCTGACTCCACTGCACCTGGCCTGCAAGATGGGAAAGCAGG
  AGATGGTGCGCGTCCTGCTGCTCTGTAATGCCCGCTGCAACATCATGGGGCCCGGTGGCTTCCCCATCCA
  CACAGCCATGAAGTTTTCCCAGAAGGGGTGTGCTGAAATGATTATCAGCATGGACAGCAACCAGATCCAC
  AGCAAGGATCCTCGCTACGGAGCCAGCCCACTCCATTGGGCCAAGAACGCCGAGATGGCCCGAATGCTGC
  TGAAGCGGGGCTGTGACGTGGACAGCACTAGCTCCTCAGGGAACACAGCCCTGCATGTGGCGGTGATGCG
  CAACCGCTTTGACTGTGTCATGGTGCTGCTGACCTACGGGGCTAATGCAGGTGCCCGCGGAGAGCACGGG
  AACACGCCACTGCACCTGGCCATGTCGAAAGATAACATGGAGATGGTCAAAGCCCTCATTGTATTTGGGG
  CAGAAGTGGACACCCCAAACGACTTTGGGGAGACTCCTGCATTGATAGCCTCCAAGATCAGCAAGCAGCT
  TCAGGATCTCATGCCCATCTCTCGAGCCCGGAAGCCAGCGTTCATCCTGAGCTCCATGAGGGACGAGAAG
  CGGAGTCACGACCACCTGCTCTGCCTGGACGGAGGGGGCGTGAAAGGCCTGGTCATTATCCAGCTTCTCA
  TCGCCATCGAGAAGGCCTCGGGAGTGGCCACCAAGGACCTCTTCGACTGGGTGGCAGGAACCAGCACAGG
  GGGCATCCTGGCCCTGGCCATTCTGCACAGTAAATCCATGGCCTATATGCGTGGTGTGTACTTCCGTATG
  AAGGACGAGGTGTTTCGGGGCTCACGGCCCTATGAGTCTGGGCCCCTGGAGGAGTTCCTGAAGCGGGAGT
  TTGGGGAGCACACCAAGATGACAGATGTCAAAAAACCCAAGGTGATGCTGACAGGGACACTGTCTGACCG
  GCAGCCAGCAGAGCTCCACCTATTCCGGAATTACGATGCTCCCGAAGCCGTTCGAGAGCCCCGCTGCAAC
  CAAAACATTAACCTGAAGCCACCGACTCAGCCTGCAGACCAACTGGTATGGCGTGCAGCCCGGAGCAGTG
  GGGCAGCCCCAACCTATTTCCGGCCCAATGGACGCTTCCTGGATGGAGGGCTGCTGGCCAACAACCCCAC
  ACTGGATGCCATGACTGAAATCCATGAGTACAATCAGGACATGATCCGCAAGGGCCAGGGCAACAAGGTG
  AAGAAACTCTCCATAGTCGTTTCTCTGGGGACAGGAAAGTCCCCTCAAGTGCCTGTAACCTGTGTAGATG
  TCTTTCGTCCCAGCAACCCTTGGGAACTGGCCAAAACTGTTTTTGGAGCCAAGGAACTGGGCAAGATGGT
  CGTGGACTGTTGCACAGATCCAGATGGGCGGGCTGTGGATCGGGCCCGGGCCTGGTGCGAGATGGTCGGC
  ATCCAGTACTTCAGACTGAACCCCCAGCTAGGGTCCGACATCATGCTGGACGAGGTCAGTGATGCAGTGC
  TGGTCAACGCCCTCTGGGAGACCGAGGTCTACATCTATGAGCACCGAGAGGAGTTCCAGAAGCTTGTCCA
  GCTGCTGCTGTCTCCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001199025
• Insert Size: 2259 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001199025.1, NP_001185954.1
• RefSeq Size: 3065 bp
• RefSeq ORF: 2259 bp
• Locus ID: 53357
• UniProt ID: P97819
• Cytogenetics: 15 E1
• Gene Summary: Catalyzes the release of fatty acids from phospholipids. It has been implicated in normal phospholipid remodeling, nitric oxide-induced or vasopressin-induced arachidonic acid release and in leukotriene and prostaglandin production. May participate in fas mediated apoptosis and in regulating transmembrane ion flux in glucose-stimulated B-cells. Has a role in cardiolipin (CL) deacylation. Required for both speed and directionality of monocyte MCP1/CCL2-induced chemotaxis through regulation of F-actin polymerization at the pseudopods (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (4) differs in the 5' UTR, compared to variant 1, and encodes isoform (1). Variants 1, 3, and 4 encode the same protein (isoform 1).