Dhx32 (NM_001286031) Mouse Untagged Clone

CAT#: MC228856

Dhx32 (untagged) - Mouse DEAH (Asp-Glu-Ala-His) box polypeptide 32 (Dhx32), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dhx32
• Synonyms: 3110079L04Rik; 4732469F02Rik; AA408140; Ddx32
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228856 representing NM_001286031
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTCCCTGCGGGAAGAAATGGATGAAGAGGAACTGGACCATCCGAACGCCTCTCCAGAGAAACGCT
  ATTTCCCCGAGTCCCTAGATTCCAGTGACGGGGACGAGGAAGGGGTCCTGGCCTGCGAGGATCTGGAGCT
  GAACCCCTTTGACGGCTTACCGTATTCATCGCGATATTACAAACTTTTGAAAGAGAGAGAAGAGCTTCCG
  ATATGGAAAGAAAAGTACTCCTTCATGGAGAGTCTGCTGCAAAACCAGGTTGTGGTGGTGTCAGGGGACT
  CCAAGTGTGGCAAGAGCTCTCAGGTTCCTCAGTGGTGTGCTGAATATTGCCTTTCCATCCATTACCAGCA
  CGGGGGCGTGATATGCACCCAGGCCCACAAACAGACGGCAGTCCAGCTGGCCCTGCGGGTGGCCGACGAG
  ATGGATGTGAATATCGGGCACGAAGTCGGCTATGTGATTCCTTTTGAGAACTGCTGCACCACTGAAACAA
  TCCTGAGGTACTGTACTGATGACATGCTGCAGAGAGAGATGATGTCCAACCCTTTCCTGGGTAGCTATGG
  CGTCATCATCTTGGACGATGTCCACGAGAGAAGCCTGGCGACTGACGTGCTGCTCGGACTCCTTAAAGAT
  GTGCTGCTGGCAAGACCAGAGCTGAAGCTCATAGTTAACTGCTCGCCTCTCCTCACCAGTAAACTCAGTT
  CCTACTATGGAGACGTGCCTGTCATAGAAGTGAGAAATAAGCACCCGGTGGAGGTGGTGTACCTTAGTGG
  GGCTCAGAAGGATTCCTTCGAGTCTGTCATACGCCTTATCTTTGAAATCCACCGCTCGGGTGAGAAGGGT
  GACGTTGTGGTCTTCCTGGCCTGTGAACAAGATATTGAAAAAACCTATGAACTTGTGTGTCAGGAAGGAT
  CCAACTTAAACCCAGATGTGGGCGACCTGGTGGTCATCCCTTTGTATCCCAAAGAGAAGTGCAGCCTGTT
  CAGGCCAGTGGACGAAACCGAGAAAAGATGCCAAGTGTATCAGAGACGGGTGGTGCTGACCACCAGCTGC
  GGGGAGTCTCTGATCTGGAGCCACACGGTCAAATTTGTCATTGATGTTGGTCTGGAAAGAAGACAGGTAT
  ATAACCCCCGGATCAGAGCCAACTCCCTCGTCCTGCAGCCCATCAGCCAAAGCCAAGCAGAGATCCGGAA
  GCAGCTCCTCGGGTCTTCTCCCTCAGGAAAACTTTTCTGTCTGTACACTGAAGAATTTGCCTCCAAAGAC
  ATGAGGCCCCTTAAGCCAGCGGAAATGCAGGAAGCCAACCTGACTAGCATGGTGCTCTTCATGAAGAGGG
  TGGACATTGCAGGCCTGGGCCGCTGCGACTTCATGAACAGGCCAGCTCCAGAAAGCCTGATGCAGGCACT
  GGAAGATTTAGATTACCTGGCAGCCCTGGACAACGATGGGAATCTCTCTGAGTTTGGAATCATCATGTCT
  GAGTTTCCTCTGGATCCACAGCTCTCAAAGTCTATCTTAGCATCCTGTGAATTTGACTGTGTAGACGAAA
  TGCTAACCATCGCTGCCATGGTAACAGCTCCCAGTTGCTTTTTGCACGTGCCTCATGGTGCTGAGGAGGC
  TGCAGTGACTTGCTGGAAAACGTTCTTGCACCCTGAAGGTGATCACTTTACGCTCATCAATGTCTACAAT
  GCCTACCAAGATACAGTTCTGAACTCTGCCAATGAACACTGTGTGGAGATGTGGTGTCACGATTGCTTCC
  TCAGCTGCTCTGCTCTCAGGATGGCAGACGTTATCCGAGCAGAACTCTTAGAGATCATCAAGCGGATCGA
  GCTTCCCTATGCAGAGCCTGCCTTTGGCTCCAAGGAAAACGGGTTGAACATCAAGAAAGCGCTGCTCTCT
  GGGTACTTCATGCAGATTGCTCGGGATGTTGACGGGTCAGGTAACTACTTGATGCTTACACATAAGCAGG
  TTGCTCAGCTGCATCCCTTATCAAGCTATTCCATCACCAAGAAGATGCCCGAGTGGGTCCTCTTCCACCA
  GTTCAGCATATCTGAGAACAACTACATCAGGGTCGCCTCCGCAGTCTCCCCTGAGCTATTTATGCAGCTG
  GTACCACAGTACTATTTCAGTAACCTGCCTCCTAGTGAAAGCAAGGACATCCTACAGCAAGCTGCAGGCC
  ACCTACCCACAGAAACTGTGAACAAGGACCAGGATGTGTGTGACAAGTGCCCAGATGCTACTGAGCAGAG
  ATGCACAATCCAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001286031
• Insert Size: 2256 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001286031.1, NP_001272960.1
• RefSeq Size: 2878 bp
• RefSeq ORF: 2256 bp
• Locus ID: 101437
• UniProt ID: Q8BZS9
• Cytogenetics: 7 F3