Mme (NM_001289462) Mouse Untagged Clone

CAT#: MC228849

Mme (untagged) - Mouse membrane metallo endopeptidase (Mme), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mme
• Synonyms: 6030454K05Rik; C85356; CALLA; CD10; NEP; SFE
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228849 representing NM_001289462
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAAGATCAGAAAGTCAGATGGATATAACTGATATCAATGCTCCAAAGCCAAAGAAGAAACAGCGAT
  GGACTCCCCTGGAGATCAGCCTCTCTGTGCTTGTCTTGCTCCTGACTATCATAGCGGTGACAATGATCGC
  TCTCTATGCAACCTATGATGATGGCATTTGCAAATCATCAGACTGCATAAAATCAGCTGCTCGACTGATT
  CAGAATATGGATGCCTCTGTTGAGCCATGTACAGACTTCTTCAAGTATGCTTGTGGAGGCTGGTTGAAAC
  GCAACGTCATTCCCGAGACCAGTTCCCGATACAGTAATTTTGACATTTTAAGAGATGAGCTAGAAGTCAT
  TTTGAAAGATGTCCTTCAAGAACCCAAAACTGAAGACATAGTAGCAGTGCAGAAAGCAAAAACCTTGTAC
  AGATCGTGTATAAATGAGTCTGCTATTGATAGCAGAGGCGGACAACCTCTACTCAAACTGTTACCAGATA
  TATATGGGTGGCCAGTAGCATCAGATAACTGGGATCAAACATATGGCACTTCTTGGACAGCTGAGAAATC
  CATTGCACAACTGAATTCTAAATATGGGAAAAAGGTCCTCATTAATTTTTTTGTTGGCACTGATGATAAG
  AATTCTACCCAGCATATAATTCATTTTGACCAGCCTCGACTTGGCCTCCCTTCCAGAGACTACTATGAGT
  GCACTGGAATATATAAAGAGGCTTGCACAGCATATGTGGATTTTATGATTTCTGTGGCCAGACTGATTCG
  TCAGGAACAAAGTTTGCCTATTGATGAAAACCAGCTCTCTTTGGAAATGAATAAAGTTATGGAATTGGAA
  AAAGAAATTGCCAATGCTACAACTAAACCAGAAGACCGAAATGACCCAATGCTGCTTTATAACAAAATGA
  CATTGGCCAAGCTGCAGAACAACTTTTCCCTGGAGGTCAATGGGAAGTCATTCAGCTGGTCAAATTTCAC
  AAATGAAATCATGTCAACTGTGAATATTAATATTCAAAATGAGGAAGAAGTTGTTGTCTATGCTCCAGAA
  TATTTAACCAAACTTAAGCCTATTCTTACCAAATATTCTCCCAGAGATCTTCAAAATTTAATGTCCTGGA
  GGTTCATAATGGATCTTGTAAGCAGCCTCAGCCGAAACTACAAGGAGTCCAGAAATGCTTTCCGCAAGGC
  CCTTTATGGGACTACATCAGAAACTGCAACATGGAGACGGTGTGCTAACTACGTCAATGGGAATATGGAG
  AATGCTGTGGGGAGGCTTTATGTGGAAGCGGCTTTTGCTGGAGAGAGCAAGCACGTGGTTGAAGACTTGA
  TTGCACAAATCCGGGAAGTTTTTATTCAGACTTTGGATGACCTTACTTGGATGGATGCTGAGACAAAAAA
  GAAAGCTGAAGAAAAGGCCTTGGCAATTAAAGAAAGGATTGGCTATCCTGATGACATCATTTCCAATGAG
  AACAAACTGAATAATGAGTATCTTGAGTTGAACTACAGGGAAGATGAATACTTTGAGAACATAATTCAAA
  ATTTGAAATTCAGCCAAAGCAAGCAGCTAAAGAAGCTCCGAGAAAAAGTGGACAAAGATGAGTGGATAAG
  TGGAGCAGCTGTAGTCAATGCATTTTATTCCTCAGGCCGAAATCAGATAGTCTTCCCCGCTGGCATTTTG
  CAGCCCCCATTCTTCAGTGCTCAGCAATCCAACTCATTGAACTATGGGGGCATCGGCATGGTCATTGGAC
  ATGAAATCACACATGGATTTGATGACAATGGAAGAAATTTCAATAAAGATGGAGACCTCGTTGACTGGTG
  GACGCAGCAGTCGGCAAATAATTTTAAAGACCAATCCCAGTGTATGGTATACCAGTATGGAAACTTTTCC
  TGGGACCTAGCAGGTGGACAGCATCTCAATGGAATTAATACACTAGGAGAAAACATTGCTGATAATGGAG
  GTATTGGCCAAGCATACAGAGCCTATCAGAATTATGTTAAAAAGAATGGTGAAGAAAAATTACTCCCTGG
  ACTTGACCTCAATCACAAACAACTATTTTTCCTGAACTTTGCCCAGGTGTGGTGTGGGACCTACCGGCCA
  GAGTATGCAGTCAATTCTATTAAAACAGATGTACACAGTCCTGGAAATTTCAGGATCATTGGGACTTTGC
  AGAACTCTGCCGAGTTTGCAGATGCCTTTCATTGCCGCAAGAATTCATACATGAATCCAGAAAGGAAATG
  TCGGGTTTGGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001289462
• Insert Size: 2253 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289462.1, NP_001276391.1
• RefSeq Size: 6088 bp
• RefSeq ORF: 2253 bp
• Locus ID: 17380
• UniProt ID: Q61391
• Cytogenetics: 3 29.97 cM
• Gene Summary: Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF) (By similarity). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers (PubMed:20876573).[UniProtKB/Swiss-Prot Function]