Sema3b (NM_001042779) Mouse Untagged Clone

CAT#: MC228843

Sema3b (untagged) - Mouse sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3B (Sema3b), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Sema3b
• Synonyms: LUCA; Se; sem; SemA; sema5; Semaa; semaV
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228843 representing NM_001042779
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCGGGCTGAGGCCGCCGCCATGATCCCAGGCCTGGCCCTTCTCTGGGTAGCAGGGCTAGGGGATA
  CTGCCCCTAACCTTCCCCGCCTTCGGCTCTCCTTTCAAGAATTACAGGCCCGGCATGGTGTCCGAACCTT
  CAGGCTGGAGCGGACCTGCTGTTATGAAGCCTTGCTGGTGGATGAGGAGCGTGGACGCCTGTTTGTGGGT
  GCTGAGAACCACGTGGCTTCCCTCAGCCTGGACAACATCAGCAAGCGAGCCAAGAAGCTGGCCTGGCCCG
  CCCCCGTGGAATGGCGTGAAGAATGCAACTGGGCAGGGAAGGACATTGGTACCGAGTGCATGAACTTCGT
  GAAGCTGCTGCACACCTACAACCACACCCACTTGCTGGCCTGTGGCACAGGGGCTTTCCACCCAACCTGT
  GCCTTTGTGGAGGTGGGCCACCGGCTGGAGGAACCCATGCTTCAACTGGACCGGAGGAAACTTGAGGACG
  GCAAGGGGAAGACTCCTTATGACCCAAGGCATCGGGCTGCCTCGGTGCTGGTGGGGGAAGAACTGTATTC
  TGGGGTGACAGCAGACCTTATGGGCCGGGACTTTACCATCTTTCGAAGCCTTGGTCAGAATCCGAGTCTC
  CGAACAGAGCCCCATGATTCCCGCTGGCTCAATGAACCCAAGTTTGTCAAGGTCTTTTGGATCCCAGAGA
  GTGAGAACCCTGATGACGATAAAATCTATTTCTTCTTCCGCGAGTCCGCTGTGGAAGCAGCACCAGCAAT
  GGGGCGCATGTCTGTGTCTCGTGTTGGCCAGATCTGCAGGAATGACCTGGGTGGCCAGCGGAGCTTGGTC
  AACAAATGGACCACATTTCTGAAGGCGCGGCTTGTGTGCTCAGTACCTGGAGTTGAGGGTGACACCCACT
  TTGACCAACTTCAGGATGTTTTCCTTCTGTCCTCCCGAGACCGCCAGACACCTCTTCTCTATGCTGTCTT
  CTCCACCTCCAGTGGTGTCTTCCAGGGCTCTGCTGTGTGCGTGTACAGCATGAACGATGTGCGCCGAGCC
  TTCTTGGGACCTTTTGCTCACAAAGAGGGGCCTACACACCAGTGGGTGTCCTACCAGGGTCGTGTCCCCT
  ACCCAAGACCTGGCATGTGCCCCAGCAAGACCTTTGGCACCTTCAGCTCCACCAAGGACTTCCCAGATGA
  CGTTATCCAGTTTGCTCGGAACCACCCTCTCATGTACAACCCAGTCCTGCCCATGGGGGGGCGCCCTCTC
  TTCCTACAAGTGGGAGCTGGGTACACCTTCACCCAAATCGCCGCAGACCGAGTAGCAGCTGCCGATGGAC
  ACTACGATGTTCTCTTCATTGGTACAGATGTGGGCACAGTGCTGAAAGTGATCTCAGTCCCCAAAGGCAG
  CCGACCTAATTCTGAAGGACTTCTCCTGGAAGAGCTGCAGGTGTTCGAGGACTCTGCCGCTATCACCAGC
  ATGCAAATCTCCTCTAAAAGGCAACAACTCTACATAGCATCGCGCAGCGCAGTGGCCCAGATTGCTTTGC
  ATCGCTGCACTGCCCTAGGCCGCGCCTGCGCAGAATGCTGCTTGGCCCGTGATCCTTACTGCGCCTGGGA
  TGGATCAGCTTGCACACGCTTCCAGCCTACGGCCAAGAGACGGTTCCGGAGGCAAGACATAAGGAATGGC
  GACCCCAGCACCCTATGCTCTGGAGACTCTTCTCACTCTGTGCTGCTGGAGAAGAAGGTGTTGGGTGTGG
  AGAGCGGCAGCGCGTTTCTGGAGTGTGAGCCCCGCTCGCTCCAGGCGCATGTGCAGTGGACCTTCCAAGG
  TGCAGGGGAGGCAGCTCACACCCAGGTGCTGGCTGAGGAGAGAGTAGAGCGCACTGCGCGGGGGCTGCTG
  TTGCGGGGGCTGCGGCGCCAGGACTCTGGCGTGTATCTTTGCGTCGCGGTTGAACAAGGCTTTTCACAAC
  CACTGCGTCGCCTGGTGCTGCATGTGTTGAGTGCGGCGCAGGCTGAACGACTGGCACGGGCAGAGGAAGC
  AGCCGCTCCTGCACCTCCTGGCCCTAAACTCTGGTACCGGGACTTTCTGCAGTTGGTGGAGCCAGGCGGT
  GGCGGAGGTGCAAACTCCCTGCGAATGTGCCGCCCGCAGCCCGGGCACCACTCTGTGGCAGCAGATTCAC
  GTCGTAAGGGTCGCAACAGACGGATGCATGTCTCTGAGCTCCGTGCTGAGCGTGGACCACGTAGTGCAGC
  TCACTGGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001042779
• Insert Size: 2250 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001042779.2, NP_001036244.1
• RefSeq Size: 3746 bp
• RefSeq ORF: 2250 bp
• Locus ID: 20347
• Cytogenetics: 9 58.31 cM
• Gene Summary: This gene encodes a secreted protein that belongs to the class 3 semaphorin/collapsin family. Members of this family play a role in growth cone guidance during neurogenesis. The encoded protein inhibits axonal extension. This protein is thought to be an osteoblast protein that regulates bone mass and affects skeletal homeostasis. A similar gene in humans functions as a tumor suppressor gene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014]
  Transcript Variant: This variant (1) differs in the 5' UTR compared to variant 3. Variants 1 through 5 encode the same protein.