Trim2 (NM_001271728) Mouse Untagged Clone

CAT#: MC228831

Trim2 (untagged) - Mouse tripartite motif-containing 2 (Trim2), transcript variant 5

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Trim2
• Synonyms: mKIAA0517; narf
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228831 representing NM_001271728
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAGTGAGGGCGCCAGCATCCCAAGTCCCGTGGTGCGCCAGATTGACAAGCAGTTTCTGATCTGCA
  GTATATGCCTGGAACGGTACAAGAACCCCAAGGTCCTGCCCTGTCTGCACACTTTCTGCGAGAGGTGCCT
  GCAGAACTACATCCCAGCCCACAGTTTAACCCTCTCCTGCCCGGTGTGTCGCCAGACATCCATCCTGCCC
  GAGAAAGGGGTGGCCGCACTTCAGAACAACTTCTTTATCACGAACCTCATGGATGTGCTGCAGCGGACGC
  CAGGCAGCAACGGCGAGGACTCTTCCATCCTGGAGACGGTCACCGCTGTGGCTGCAGGAAAGCCTCTTTC
  GTGCCCAAACCATGATGGAAATGTGATGGAATTTTACTGCCAGTCCTGTGAGACCGCCATGTGTCGGGAG
  TGCACGGAGGGGGAGCACGCGGAGCACCCCACAGTCCCGCTGAAGGATGTGGTGGAGCAGCACAAGGCCT
  CGCTCCAGGTCCAGCTGGACGCAGTCAACAAAAGACTCCCAGAAATAGATTCTGCTCTTCAGTTCATCTC
  AGAAATCATCCATCAGTTAACCAACCAAAAGGCCAGCATTGTGGATGACATCCACTCCACCTTTGATGAG
  CTGCAGAAGACTTTAAATGTTCGCAAGAGCGTGCTGCTCATGGAGTTGGAGGTCAACTATGGTCTCAAAC
  ACAAAGTGCTGCAGTCGCAGCTGGATACCCTGCTCCAAGGCCAGGAAAGCATTAAGAGCTGCAGCAACTT
  CACGGCTCAGGCCCTCAACCATGGCACGGAGACGGAGGTGCTGCTGGTGAAGAAGCAGATGAGCGAGAAG
  CTGAACGAACTGGCGGACCAGGACTTCCCACTGCACCCGCGCGAGAACGACCAGCTGGATTTTATCGTGG
  AAACGGAGGGACTGAAGAAGTCTATCCACAACCTGGGGACCATCCTCACCACTAACGCGGTCGCCTCTGA
  GACGGTGGCCACGGGGGAGGGGCTGCGGCAGACCATCATCGGGCAGCCCATGTCCGTTACTATAACCACC
  AAGGACAAAGACGGAGAGCTGTGCAAGACAGGCAACGCATACCTCACCGCTGAGCTCAGCACCCCGGACG
  GCAGCGTGGCCGACGGAGAGATCCTGGACAACAAGAACGGCACCTACGAGTTCTTGTATACTGTGCAGAA
  GGAGGGGGACTTCACCCTGTCCCTCAGGCTCTACGACCAGCATATCCGAGGCAGCCCGTTTAAGCTCAAG
  GTCATCCGCTCTGCCGACGTGTCCCCCACCACCGAGGGCGTGAAGCGACGAGTCAAGTCCCCGGGGAGCG
  GGCACGTCAAGCAGAAGGCTGTGAAGAGACCTGCCAGCATGTACAGCACCGGCAAGCGAAAAGAGAACCC
  CATCGAGGACGACCTGATCTTCCGAGTCGGTACCAAAGGAAGAAACAAAGGAGAATTTACAAACCTGCAG
  GGTGTGGCTGCATCTACAAGTGGAAAGATATTAATTGCAGATAGTAACAACCAGTGTGTGCAGATCTTTT
  CCAATGATGGACAGTTCAAAAGTCGTTTCGGCATCCGAGGACGATCACCCGGACAGCTCCAGCGGCCCAC
  GGGGGTAGCGGTGCACCCCAGTGGGGACATCATCATTGCAGATTATGACAACAAGTGGGTTAGCATTTTC
  TCCAATGATGGGAAGTTTAAGACAAAAATTGGATCAGGAAAACTGATGGGGCCCAAAGGAGTTTCTGTGG
  ACCGCAATGGGCACATTATTGTGGTGGACAACAAGGCGTGCTGTGTGTTTATCTTCCAGCCCAACGGGAA
  AATAGTCACCAGGTTTGGTAGCCGAGGAAACGGGGACAGGCAGTTTGCAGGTCCCCACTTTGCAGCTGTG
  AATAGCAATAATGAGATCATCATTACAGATTTCCATAATCACTCTGTCAAGGTATTTAATCAGGAAGGAG
  AATTCATGTTGAAGTTTGGCTCCAATGGAGAAGGAAATGGGCAGTTTAATGCACCAACAGGTGTGGCCGT
  GGACTCAAATGGGAACATCATTGTGGCTGACTGGGGGAACAGCAGGATCCAGGTTTTTGATGGGAGTGGA
  TCATTTTTGTCCTATATCAACACATCTGCTGACCCGCTCTATGGCCCCCAGGGCCTGGCTCTCACTTCAG
  ATGGCCATGTTGTGGTTGCAGACTCCGGAAACCACTGTTTCAAGGTCTACCGGTACTTACAGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001271728
• Insert Size: 2235 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001271728.1, NP_001258657.1
• RefSeq Size: 7211 bp
• RefSeq ORF: 2235 bp
• Locus ID: 80890
• UniProt ID: Q9ESN6
• Cytogenetics: 3 F1
• Gene Summary: UBE2D1-dependent E3 ubiquitin-protein ligase that mediates the ubiquitination of NEFL and of phosphorylated BCL2L11. Plays a neuroprotective function. May play a role in neuronal rapid ischemic tolerance.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (5) contains a distinct 5' UTR and lacks an in-frame portion of the 5' coding region, compared to variant 1. The resulting isoform (2) has a shorter N-terminus, compared to isoform 1. Variants 2, 3, and 5 encode the same isoform (2).