Trim3 (NM_001285871) Mouse Untagged Clone

CAT#: MC228828

Trim3 (untagged) - Mouse tripartite motif-containing 3 (Trim3), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Trim3
• Synonyms: BERP1; HAC1; Rnf22
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228828 representing NM_001285871
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAAAGAGGGAGGACAGCCCTGGCCCGGAGGTGCAGCCAATGGACAAGCAGTTTCTGGTATGCAGTA
  TCTGTCTGGATCGGTACCGGTGCCCCAAAGTTCTGCCTTGTCTACATACCTTCTGTGAAAGATGCCTCCA
  GAACTACATCCCTCCTCAGAGCCTGACACTGTCCTGTCCAGTGTGCCGGCAGACATCCATCCTCCCAGAA
  CAGGGCGTCTCAGCCCTACAAAACAACTTCTTCATCAGCAGCCTCATGGAGGCCATGCAGCAGGCACCTG
  AGGGGGCTCATGACCCTGAAGACCCCCACCCCCTCAGCGCAGTGGCTGGCCGCCCTCTCTCCTGCCCCAA
  CCATGAAGGCAAGACAATGGAGTTTTACTGTGAGGCCTGTGAGACTGCCATGTGTGGTGAGTGCCGCGCA
  GGGGAGCACCGTGAACACGGCACAGTGCTGTTGCGGGATGTGGTGGAGCAGCACAAGGCAGCTCTGCAGC
  GCCAGCTTGAGGCTGTGCGTGGCCGATTGCCACAGCTGTCTGCAGCTATCGCCTTGGTTGGGGGTATCAG
  CCAGCAGCTGCAAGAGCGCAAGGCAGAGGCCCTGGCTCAGATAAGTGCTGCCTTTGAGGACCTGGAGCAA
  GCTCTGCAGCAGCGCAAGCAGGCTCTGGTCAGCGACTTGGAGAGCATTTGTGGGGCCAAACAGAAGGTGT
  TGCAGACACAGTTAGACACACTGCGCCAGGGCCAGGAGCACATTGGCAGTAGCTGCAGCTTTGCAGAGCA
  GGCACTGAGACTGGGCTCTGCCCCTGAGGTTTTGCTAGTAAGAAAGCATATGCGAGAGAGGCTGGCTGCT
  CTGGCAGCTCAGGCCTTCCCAGAAAGGCCACATGAGAATGCCCAGCTGGAACTGGTCCTTGAAGTGGACG
  GGTTGCGGAGATCGGTGCTCAACCTGGGGGCACTGCTCACCACCAGTGCTACAGCCCATGAGACAGTGGC
  CACGGGCGAGGGCCTGCGCCAGGCACTAGTTGGCCAGCCTGCTTCTCTCACTGTCACCACCAAAGACAAA
  GATGGGCGGCTGGTGCGCACAGGCAGCGCAGAGCTGTGTGCAGAGATCACTGGCCCCGATGGTGTGCGCC
  TTGCAGTACCGGTGGTGGACCACAAAAATGGCACATACGAGCTGGTGTACACTGCACGCACAGAAGGTGA
  CCTGCTCCTCTCAGTGCTGCTCTATGGACAGCCGGTGCGTGGCAGCCCTTTTCGTGTGCGTGCCCTTCGA
  CCTGGGGACCTGCCACCTTCCCCAGATGATGTGAAGCGCCGGGTCAAGTCTCCCGGTGGTCCTGGCAGTC
  ATGTGCGCCAGAAGGCAGTTCGTAGGCCGAGCTCCATGTACAGCACTGGTGGCAAACGGAAGGACAATCC
  AATTGAAGACGAACTCGTCTTTCGTGTTGGTAGTCGTGGAAGGGAGAAGGGTGAATTCACCAATTTACAA
  GGTGTGTCCGCTGCTAGCAGTGGCCGCATCGTGGTAGCAGACAGCAACAACCAATGTATTCAGGTTTTCT
  CCAATGAGGGCCAGTTCAAGTTCCGATTTGGGGTCCGTGGGCGCTCACCTGGGCAACTACAGCGTCCTAC
  AGGTGTGGCAGTGGACACCAATGGAGACATTATTGTGGCAGACTATGACAACCGTTGGGTCAGCATCTTC
  TCTCCTGAAGGCAAGTTCAAGACCAAGATTGGAGCTGGCCGCCTCATGGGACCCAAGGGAGTGGCTGTGG
  ACCGAAATGGACATATCATTGTGGTGGATAACAAATCTTGCTGTGTCTTCACCTTCCAGCCCAATGGCAA
  GCTTGTTGGGCGTTTTGGAGGCCGTGGGGCCACTGACCGCCACTTTGCAGGGCCCCACTTTGTGGCTGTG
  AACAACAAGAATGAGATTGTAGTAACGGATTTCCATAACCATTCAGTGAAGGTGTACAGTGCTGACGGAG
  AGTTCCTCTTCAAATTTGGCTCGCATGGCGAGGGCAACGGACAGTTCAATGCCCCCACGGGAGTAGCTGT
  GGATTCCAATGGGAACATCATCGTGGCCGACTGGGGCAACAGCCGCATACAGGTATTCGACAGCTCTGGT
  TCCTTCCTGTCCTATATCAACACGTCTGCAGAGCCACTATATGGCCCACAGGGCTTGGCATTGACCTCGG
  ATGGCCACGTGGTAGTGGCTGATGCTGGCAACCACTGCTTTAAGGCCTATCGATACCTCCAGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001285871
• Insert Size: 2235 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001285871.1, NP_001272800.1
• RefSeq Size: 3077 bp
• RefSeq ORF: 2235 bp
• Locus ID: 55992
• UniProt ID: Q9R1R2
• Cytogenetics: 7 E3
• Gene Summary: Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation (By similarity). Positively regulates motility of microtubule-dependent motor protein KIF21B (PubMed:24086586).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) differs in the 5' UTR and uses an alternate in-frame splice junction compared to variant 5. The resulting isoform (a) has the same N- and C-termini but is shorter compared to isoform c. Variants 1, 2, and 3 all encode the same isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.