Palb2 (NM_001289844) Mouse Untagged Clone

CAT#: MC228824

Palb2 (untagged) - Mouse partner and localizer of BRCA2 (Palb2), transcript variant 4

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Palb2
• Synonyms: BC066140
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228824 representing NM_001289844
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGAGCTTTCCGGGAAGCCCCTCAGCTATGCGGAGAAGGAAAAGTTGAAGGAAAAGCTAGCATTTT
  TGAAAAAGGAATACAGCAGGACACTTGCTCGACTTCAGCGTGCCAAAAGAGCTGAGAAGGCTAAGAACTC
  TAAGAAAGCCATAGAAGATGGTGTGCCCCAGCCAGAAGCTTCCTCACAGCTAAGCCACTCTGAATCTATA
  AATAAAGGCTTTCCTTGTGACACATTGCAAAGCAACCATCTGGATGAGGAGACTGGAGAAAACATCTCTC
  AGATACTTGATGTTGAACCTCAATCCTTTAACTGTAAACAAGGCAAAGAAGTATTACATACACCAAGAGC
  AGGTGACATTCAAGGACAACTTTTGCATAGCACCAGCAGCCCTGATGGCAAGAAAGAGCAGAACACGCTT
  CCGGGGACAACAAAGACGCCGTGGGAGAAGTCATCTGTTTCACAGGAGAAGGAAGATTATTTTGACACTA
  ATTCTCTGGCGCTCCTTGACTTTGAGTTACCTGATGAAGACTTTGGGCTTCTTAAACTTGAAAAATTGAA
  GTCCTGCTCGGAAAAACTGATTGAGTCTCCTGACTCAAAAAACTGTGGTGAGAGGCTTCCTAGAGAAGGA
  AACCATGCTGCTCTGGAGGAACTGCAAAGAGATTCGGAGACGGAGGGCTTGGAAGAGGAGCTCACTGTTC
  CACCAGGAGAAGCGTACCGTCCAGGGCCAACCCTGAGAAGGCAGCCAGGGAGCAAGGACCTTTCTTCATC
  CATAGTGCTTTTCACTCCTGCAGACACTGCTGCGCCTAACGACAGTGGCAGGCCTCCTCCCTCCCTGTGC
  TCACCTGCTTTCCCCATCTTAGGCATGACTCCAGCTCTTGGCTCCCAAGCAGCCGGTGAGACTCTATCTA
  CTGAAGCTGCACAGCCTTGCTCTACATCCCAACCTCCTCTCTTGGGAGACACAAACAGTCTTGTCAATAA
  CAGTAAACAGTGCAACAGTTCAGCCTGCTCACCAAAACCGGACACCAACCTGCAAGCGTCAGGTAGGCAA
  GGACAACCTGCCTGTGACAGTGACTCTGGCCCCCAAGCAACACCTCTACCTGTTGAGTCATTCACTTTCA
  GAGAAAATCAGCTTTGTGGAAATGCATGCCTCGAGTTACATGAACATTCCACTGAACAGACTGAAACTGC
  AGATCGCCCTGCTTGTGACAATCTAAACCCAGGAAACCTACAGTTGGTTTCAGAGTTAAAGAATCCTTCC
  AGTTCCTGCTCTGTGGATGTGAGCGCCATGTGGTGGGAAAGAGCTGGTGCTAAGGAGCCATGTATCGTAA
  CTGCTTGTGAAGATGTAGTTTCTCTTTGGAAACCCTTGAATTCTCTGCAGTGGGAGAAAGTTCATACCTG
  GCACTTCACAGAGGTTCCAGTATTACAAATAGTTCCAGTGCCTGATGTTTACAATCTTATATGTGTAGCT
  TTGGGAAGTTTGGAAATCAGAGAAATCAGGGCGTTGCTGTGCTCTTCTGGTGATGACAGTGAAAAGCAAG
  TCCTCCTGAAATCTGGAGATATAAAAGCTATGCTTGGCCTGACAAAGCGGAGGCTAGTTAGTAGCACTGG
  GACCTTTTGCAATCAACAAATACAAATCATGACATTTGCTGACGACGGAAGCAGCAAAGATGAACAGCTT
  TTGATGCCTCCTGATGAGACTGTACTGACTTTTGCTGAAGTCCAAGGGACGCAGGAAGCTCTGCTTGGTA
  CTACCACTGTGAACAGCATTGTGATCTGGAATTTAAAAACCGGCCAGCTCCTGAAGAAGATGCACATTGA
  TGACTCCTACCAGGCTTCGGTCTGTCACGGAGCCTATTCTGAGAAGGGGCTCCTGTTTGTTGTTGTGAGT
  CAACCTTGTGCCAAAGAGAGCCAGGCCCTTGGAAGCCCTGTGTTCCAGCTGCTGGTGATTAACCCTAAGA
  CGGCCCAGAGTGTGGGTGTTCTGCTGTGTTCCCTTCCTCAGGGGCAGGCTGGAAGGTTCCTGGAAGGGGA
  TGTGAAAGATCATGTCGCAGCAGCAGTCCTGACTTCTGGGACAATTGCCATTTGGGATTTGCTTCTGGGT
  CACTGCACGGCTCTCCTCCCACCTGTCTCTGACCAGAGTTGGTCTTTGGTTAAATGGTCGGGTACAGATT
  CTCACTTGCTAGCTGGACAAAAGGATGGAAATATATTTATATACCGATACTTTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001289844
• Insert Size: 2226 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289844.1, NP_001276773.1
• RefSeq Size: 2661 bp
• RefSeq ORF: 2226 bp
• Locus ID: 233826
• UniProt ID: Q3U0P1
• Cytogenetics: 7 F2
• Gene Summary: Plays a critical role in homologous recombination repair (HRR) through its ability to recruit BRCA2 and RAD51 to DNA breaks. Strongly stimulates the DNA strand-invasion activity of RAD51, stabilizes the nucleoprotein filament against a disruptive BRC3-BRC4 polypeptide and helps RAD51 to overcome the suppressive effect of replication protein A (RPA). Functionally cooperates with RAD51AP1 in promoting of D-loop formation by RAD51. Serves as the molecular scaffold in the formation of the BRCA1-PALB2-BRCA2 complex which is essential for homologous recombination. Via its WD repeats is proposed to scaffold a HR complex containing RAD51C and BRCA2 which is thought to play a role in HR-mediated DNA repair. Essential partner of BRCA2 that promotes the localization and stability of BRCA2. Also enables its recombinational repair and checkpoint functions of BRCA2. May act by promoting stable association of BRCA2 with nuclear structures, allowing BRCA2 to escape the effects of proteasome-mediated degradation. Binds DNA with high affinity for D loop, which comprises single-stranded, double-stranded and branched DNA structures. May play a role in the extension step after strand invasion at replication-dependent DNA double-strand breaks; together with BRCA2 is involved in both POLH localization at collapsed replication forks and DNA polymerization activity (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (4) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. It encodes isoform 4, which lacks an internal segment and is shorter, compared to isoform 1.