Golga1 (NM_001290649) Mouse Untagged Clone

CAT#: MC228810

Golga1 (untagged) - Mouse golgi autoantigen, golgin subfamily a, 1 (Golga1), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Golga1
• Synonyms: 0710001G09Rik; 2210418B03Rik; AW107649; awag; Golgi97; golgin-97
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228810 representing NM_001290649
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTGCAAAACTAAAGAAGAAAATTGCAGAAGAGACTGCTGTTGCTCAGAGACCAGGCGGCACAACAA
  GGATCCCACGCTCTGTGAGCAAGGAGTCTGTGGCCTCCATGGGAGCTGACTCTGGAGATGATTTTGCTTC
  CGATGGTAGCAGCTCCAGAGAAGATCTCTCATCCCAGCTTTTGAGAAGAAATGAACAGATACGGAAGTTA
  GAGGCCAGACTTTCTGCTTCCATGCGGAAATTTCAAGAACAGAATGAAACATTCCAAGCCAGCAGAGCCA
  AAATGGCAGAAGGACTGGCTTTGGCTTTAGCCAGGAAGGACCAGGAATGGTCAGAAAAGATGGAACAGCT
  TGAAAAGGATAAAAGGTTTCTAACATCTCAGCTGCAAGAAGTAAAGAACCAGAGCTTGAGCCTTTTTCAA
  AAAAGAGATGAAATTGATGAATTAGAGGGGTTCCAGCAGCAGGAAATCAGCAAAGTAAAACACATGCTTT
  TAAAAAAAGAAGAATGCTTGGGGAAGATGGAGCAAGAATTGGATGCACGAACCAGGGAACTTAACCGTAC
  TCAGGAGGAGTTGGTGACTTCTAATCAGTTGTCATCTGACTTAAACGAGCGGCTAGAAGAATTGCAGAGA
  CACTGCTCAACGCTGGAAGAGCAGAGAGATCACTTGACAGCTTCAAAAGCAGGTGCAGAACATAAGATTG
  TAGTCCTGGAACAAAAGGAACAAGAGCTCCAGGCGATCATCCAGCAGCATTCCATTGACCTACAAAAGGT
  CACTGCTGAAACGCAAGAAAAAGAAAAAGTCATCACACATCTACAAGAGAAGGTTATATTCTTGGAAAAG
  AGACTAGAACAGAATTTATCAGGAGAAGATCATGTACAAGAACTCTTGAAAGAGAAAACAGTTGCTGAAC
  AGAATCTGGAAGATACTAGGCAGCAGCTCTTGGCTGCCAGGAACAGCCACACCAAGGCACTTTACCTCCT
  GGAGACTCGGGTGAAGGACCTAGAGCGGAGTCTACAGGCTGCTGAAGAACAGCTAAGCCAGAGTAGAAAT
  GTTGTGGCTGACCAGGAAGCCCAGATCCAGAAGCTTATCACTACAAACCAGGAGAACAGCCTTTCCCAGC
  AGCAGGTTCTTGCCCTGGAACAGCATTGCAGGGAGCGCATCCATGCACTGGAGGCCCAAATTGAAGCTCT
  GGAACAAACACGGGTAGCTGACCAGATAGCCTCAGAGCAGGGAATGCTACAACTGCAGCAGGAAAATGTG
  GCCCTTAAAGAAAGCAGAAATGAATGTGAACATTCCTTACAACATCACCAATTGGAACTAAAGAAACTAA
  AGGATGAATGGAGCCAAAGAGAAATTGTGAGTGTAGCAATGGCTCAAGCCCTGGAGGAGGTGCGGAAACA
  GAGAGAAGAGTTCCAGCAACAGGCCACTGAGTTGACAGCCATCATAGAAGAGAAAAATCAGAGCCTGTGT
  GAAAAGGATGAGGCACTTCTCCAGAAGGAGCAGGAACTTCGACAGCTGGAAAAAGGTCACAGTTCTGCCC
  TGCTGCAGATGCACAAACTGCAGAGGGAACTAGAGGCCTTGAAGACTTGCAAGGCTCAGGAGGCAATGCC
  AGCCACAACAGGAGAGGACTGCCTGCCACTGCAGGGCCAGGAGCCACTTGTCATTTCAAAAGCCATGCAG
  AATTCTGAGTATGAGCTTCCAGCTGCAGAAGGAACTCCAAACGGTGAGGTTGGAGCCAGTGATCTAAAGC
  AGCTACAGAAAGAAAAGCAGGACTTAGAACAGCAACTTATCGAGAAAAATAAGATTATGAAGCAAATGCA
  GCAGAGGATGCTGGAACTTAAGAAGACTCTACAGAAAGAGTTGAAAATCAGACCTGACAGTGAGCTTTTT
  GAGGTCCGGGAGAAGACAGGCCCTGAGATACCAAATATGGCCCCTTCTGTCACTAATAATACTGACCTGA
  CTGATGCCCGAGAGATCAACTTTGAATACCTTAAACATGTTGTTTTAAAATTCATGTCCTGTCGAGAATC
  TGAGGCTTTTCATCTCATAAAAGCAGTGTCAGTGCTGCTGAATTTCTCACAAGAAGAGGAGAATATGCTC
  AAAGAGACTCTGGAATATAAGATGTCTTGGTTTGGATCCAAACCAACTCCCAAGGGCAGCATCCGGCCAT
  CTATCTCCAACCCTCGGATACCATGGTCCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001290649
• Insert Size: 2202 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001290649.1, NP_001277578.1
• RefSeq Size: 4793 bp
• RefSeq ORF: 2202 bp
• Locus ID: 76899
• UniProt ID: Q9CW79
• Cytogenetics: 2 B
• Gene Summary: Involved in vesicular trafficking at the Golgi apparatus level. Involved in endosome-to-Golgi trafficking.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1, resulting in an isoform (b) that is shorter than isoform a.