Slc6a15 (NM_001252330) Mouse Untagged Clone

CAT#: MC228795

Slc6a15 (untagged) - Mouse solute carrier family 6 (neurotransmitter transporter), member 15 (Slc6a15), transcript variant b

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Slc6a15
• Synonyms: AA536730; AI326450; AI326451; v7-3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228795 representing NM_001252330
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTAAGAATAGCAAAGTGGTAAAGAGAGATTTGGATGATGATGTCATTGAATCTGTCAAAGACCTCC
  TGTCCAACGAAGACTCAGTGGAAGAGGTTTCTAAGAAGAGTGAACTAATTGTCGATGTTCAGGAAGAGAA
  AGATACAGATGCTGAAGATGGATCTGAAGCTGATGATGAAAGGCCAGCCTGGAACAGTAAGCTGCAATAC
  ATTCTGGCCCAAGTTGGATTTTCTGTAGGTCTGGGGAATGTGTGGCGGTTCCCATACCTCTGCCAAAAGA
  ATGGTGGTGGTGCATATCTTTTGCCGTATTTAATACTACTTCTGGTGATAGGAATTCCACTCTTTTTCCT
  GGAACTTTCTGTGGGTCAAAGAATTCGAAGAGGCAGCATTGGGGTTTGGAATTACATAAGCCCTAAATTG
  GGTGGGATTGGCTTCGCAAGCTGTGTAGTGTGCTATTTTGTGGCTCTCTACTACAATGTCATCATTGGCT
  GGACATTATTTTACTTTTCTCAGTCTTTTCAACAACCTCTTCCTTGGGATCAATGCCCCCTGGTGAAAAA
  CGCATCTCATACTTATGTTGAGCCAGAATGTGAACAAAGTTCTGCCACCACCTATTACTGGTACCGGGAA
  GCACTGGATATCACCAGCTCCATCTCTGACAGTGGAGGCTTAAACTGGAAGATGACGGTCTGCCTTCTGG
  TTGCCTGGGTCATGGTTTGTCTGGCCATGATCAAAGGCATTCAGTCCTCTGGAAAAATCATGTATTTCAG
  TTCTCTGTTCCCCTACGTGGTCCTTATATGTTTCCTAATAAGATCTCTCCTTTTAAATGGTTCAATTGAT
  GGCATCCGACACATGTTCACTCCTAAGCTGGAAATGATGTTGGAGCCCAAGGTTTGGAGAGAGGCAGCAA
  CTCAGGTGTTCTTTGCATTGGGTTTGGGATTTGGTGGAGTCATCGCGTTTTCAAGTTATAACAAGAGAGA
  CAACAACTGCCACTTCGATGCTGTCCTAGTGTCTTTCATCAACTTTTTCACTTCAGTTCTGGCAACACTG
  GTGGTGTTTGCAGTTCTGGGGTTCAAAGCCAATATCGTAAATGAAAAATGCATTTCACAAAACTCCGAGA
  TGATCTTGAAACTTTTGAAAATGGGAAACATTAGTTGGGATGTCATTCCCCATCACATCAACCTCTCAGC
  TGTCACTGTGGAAGATTATCGTTTAGTTTACGACATCATTCAAAAAGTGAAGGAGGAGGAGTTTGCTGTT
  CTTCATCTCAACGCCTGTCAAATTGAGGATGAGCTAAATAAAGCTGTGCAGGGCACTGGCTTGGCTTTCA
  TTGCCTTTACAGAGGCCATGACACATTTCCCTGCATCTCCTTTCTGGTCAGTGATGTTTTTCCTCATGTT
  GATAAATCTCGGCCTCGGCAGCATGTTTGGAACAATTGAAGGGATCATCACTCCTATTGTGGATACCTTC
  AAAGTGAGGAAGGAAATACTCACTGTTATCTGTTGCCTCCTGGCATTTTGTATTGGCTTGATATTCGTGC
  AGCGCTCTGGAAATTACTTCGTGACAATGTTTGACGATTATTCTGCTACATTGCCTCTGCTAATTGTGGT
  CATCTTGGAGAATATTGCCGTGAGCTTTGTTTATGGCATAGATAAGTTTATAGAAGACCTCACAGACATG
  TTAGGATTTGCTCCAAGCAAATATTACTATTATATGTGGAAATACATTTCTCCTCTAATGCTACTAACGT
  TGCTAATAGCTAGCATTGTGAATATGGGATTAAGTCCTCCCGGATATAATGCATGGATCAAGGAGAAGGC
  ATCGGAAGAATTTCTGAGCTACCCGATGTGGGGGATGGTCGTCTGTTTCTCTCTGATGGTGCTGGCCATA
  CTTCCTGTCCCAGTGGTGTTCATCATTCGTCGCTGCAACCTCATAGATGATAGTTCTGGTAACTTGGCCT
  CCGTGACCTATAAGAGAGGAAGAGTCCTGAAGGAACCTGTGAACTTGGAAGGAGATGACGCAAGCCTCAT
  TCATGGAAAGATACCGAGTGAAATGTCATCTCCAAATTTTGGTAAAAATATTTATCGAAAACAAAGTGGT
  TCCCCAACACTGGATACTGCTCCCAATGGACGCTATGGGATTGGGTATTTGATGGCAGACATGCCAGATA
  TGCCAGAGTCTGACTTGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001252330
• Insert Size: 2190 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001252330.1, NP_001239259.1
• RefSeq Size: 3563 bp
• RefSeq ORF: 2190 bp
• Locus ID: 103098
• UniProt ID: Q8BG16
• Cytogenetics: 10 D1
• Gene Summary: Functions as a sodium-dependent neutral amino acid transporter. Exhibits preference for methionine and for the branched-chain amino acids, particularly leucine, valine and isoleucine. Mediates the saturable, pH-sensitive and electrogenic cotransport of proline and sodium ions with a stoichiometry of 1:1. May have a role as transporter for neurotransmitter precursors into neurons. In contrast to other members of the neurotransmitter transporter family, does not appear to be chloride-dependent (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (b) differs in the 5' UTR, compared to variant c. Variants a, b and c encode the same protein.