Mtif2 (NM_001282118) Mouse Untagged Clone

CAT#: MC228786

Mtif2 (untagged) - Mouse mitochondrial translational initiation factor 2 (Mtif2), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mtif2
• Synonyms: 2310038D14Rik; 2410112O06Rik; IF-2(Mt); IF-2mt; IF2(mt)
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228786 representing NM_001282118
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACCAGAAGCTACTGAAGTTGGAAAACTTGCTACGATTTCATACTATTTGTAGGCAGGTGCACAGCC
  CAAGTCAAAGAAGACTCTTAGCATGGTGTAGACATGGGTTTGCACCGGCTTCCTCAGTGTGGAGAGATCT
  GCTGGGTGCCCGGTCCTGGCAGACAGACATGCTCATTGGTTCAGCTTTGCATCAGCACAGACTTCTTGTA
  ACAAAGAAGGAAAAAAGACCACCAAGATCTCAGTTGTCTCCAGTAAAAACTAAAAAAGAGGTAGAAGTAT
  GGGTTGGAATGACTGTTGAAGATCTAGCCAGTGCCATGGCAAAAGATATAGATTGTGTATACGAAGCTTT
  ATTGAACACTGCCATTGACGTAGATTCATTAGAAGCAAACTCACATTTAGATGAAGTCTGGATCAAAGAA
  GTTATAAAGAAGGCAGGAATGAAGCTGAAATGGAGCAAATTAAAACAGGAAAGAATTAGAGAAAATAAAG
  ATGCTGTGAGAAGGCCTGGGACAGATCCAGCTTTATTAAAACCAAGGTCCCCAGTTGTTACTGTAATGGG
  CCATGTTGATCATGGGAAAACGACCTTACTTGACAAACTTCGAGAAACTCAAGTTGCAGCGATGGAAGTC
  GGAGGCATCACTCAACACATTGGTGCTTTTCTTGTCTCTCTGCCTTCTGGAGAAAAGATAACTTTTCTTG
  ATACTCCTGGACATGCTGCCTTCTCAGCAATGAGAGCCAGAGGAGCTCAGGTCACCGACATTGTTGTGTT
  GGTTGTAGCTGCAGATGATGGGGTAATGAAACAAACTGTGGAATCCATTCAGCATGCAAAAGATGCAGAA
  GTTCCTATTATCCTTGCAATCAATAAGTGCGACAAGACAGATGCTGATCCTGAAAAGGTGAAAAAAGAGC
  TCCTAGCTTACGATGTGGTGTGTGAAGAGTATGGTGGTGATGTTCAAGCAGTGCACGTCTCTGCACTTAC
  GGGCGATAACCTGATGGCTTTGGCAGAAGCAACAATTGCTCTCGCAGAAATCTTGGAACTGAAAGCAGAT
  CCCACCGGTCCAGTGGAAGGAACAGTAATAGAGTCTTTCACAGACAAAGGAAGAGGTCCTGTTACAACAG
  CTATAATTCAAAGAGGAACTCTGAGAAAAGGCTCAATTCTAGTTGCTGGGAAGAGTTGGGCAAAAGTTCG
  ACTAATATTTGATGAAAATGGAAAAATACTTAATGAGGCCTATCCCAGCATGCCAGTGGGAATCATAGGC
  TGGAGAGACCTTCCTTCTGCAGGAGATGAAATTCTTGAAGTAGAATCTGAGCCAAGGGCCCGTGAAGTTA
  TTGAATGGAGGAAGTCTGAGCAAAAAGAAGAAAAAGGCAAAGATGACCTGAAAATAATGGAAGAAAAGCG
  AAGGGAACACCAAGAAGCGCATCGGAAAGCCCGTGAGAAGTATGGCAGTCTGCACTGGAAAGAGAGATCA
  TATATAAAGTTCCTTGAAAGGAAACAACAGAGACCCTTAAAGCCCAAAGAAAAGGTAGAAAGGCAATCAA
  ATGTACTTCCTATAATTATTAAAGGTGACGTTGATGGGTCTGTGGAGGCCATCTTGAACCTTCTGGATAC
  CTACGATGCTTCCCACGAATGTGAACTCGAATTAGTACATTTTGGATTGGGTGACATCAGTGAAAATGAT
  GTTACCTTTGCTGAGACATTTGATGGTGTTATTTATGGTTTTAATGTGGAGGCAGGCAGTGCTATTCAGC
  AGTCAGCTGCACAAAAGGGAGTTAAGATTAAACTTCACAAAATCATCTACCATCTTATTGAAGATTTGCA
  GGAGGAACTAAGCAGCAGATTGCCCCACACACTGGAGGAGTACCCAATAGGTGAGGCTTCTATACTAGCT
  ACCTTCACTGTAACAGAAGGGAAGAAAAAAATTCCTGTTGCTGGCTGCAGAGTTCAAAAGGGACAATTAG
  AAAGACATAAGAAGTTTAAATTAATCCGAAACGGCCAAGTTATTTGGAAGGGATCATTAACCTCTCTGAA
  ACACCATAAAGATGACATTTCAGTTATAAAAACTGGTATGGACTGTGGTCTTAGTTTAGATGAAGAAAAA
  GTCGAATTCAAACCGGGAGATCAAGTCATTTGTTATGAAGAAAATAAAGTTCCAACTAAGACTTCCTGGG
  ATCCAGGATTTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001282118
• Insert Size: 2184 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001282118.1, NP_001269047.1
• RefSeq Size: 2739 bp
• RefSeq ORF: 2184 bp
• Locus ID: 76784
• UniProt ID: Q91YJ5
• Cytogenetics: 11 A3.3
• Gene Summary: One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Both variants 1 and 2 encode the same isoform (1).