Tlk2 (NM_001294331) Mouse Untagged Clone

CAT#: MC228760

Tlk2 (untagged) - Mouse tousled-like kinase 2 (Arabidopsis) (Tlk2), transcript variant F

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tlk2
• Synonyms: 4933403M19Rik; PKU-alpha; PKUalpha; Tlk
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228760 representing NM_001294331
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGGAAGAACTGCATAGCCTGGACCCACGAAGGCAGGAATTACTAGAGGCCAGGTTCACTGGAGTTG
  GTGTAAGTAAGGGGCCACTCAACAGTGAGTCTTCCAACCAGAGTCTGTGCAGCGTGGGGTCGTTGAGTGA
  TAAAGAAGTAGAGACTCCTGAGAAAAAGCAGAATGACCAGCGAAATCGGAAAAGGAAAGCCGAGCCATAT
  GACACTAGCCAAGGGAAAGGCACTCCTAGGGGACATAAAATTAGTGATTACTTTGAGCGTCGAGCAGAAC
  AGCCTCTGTATGGTTTAGATGGCAGTGCAGCAAAGGAGGCCTCAGAAGAGCAGTCTGCTCTGCCCACCCT
  CATGTCAGTGATGTTAGCAAAACCGCGACTTGACACAGAGCAGTTAGCGCCACGGGGAGCTGGCCTCTGC
  TTCACTTTCGTCTCTGCTCAACAAAACAGTCCTTCTTCTACGGGTTCTGGCAATACAGAACATTCTTGCA
  GCTCCCAAAAACAGATCTCCATCCAGCACAGACAGACCCAGTCTGACCTCACAATAGAAAAAATATCTGC
  ACTAGAAAACAGTAAGAACTCTGACTTAGAGAAGAAGGAAGGAAGAATAGATGATTTATTAAGAGCCAAC
  TGTGATTTGAGACGACAGATAGATGAACAGCAAAAGATGCTAGAGAAATACAAGGAACGATTAAATAGAT
  GTGTCACCATGAGCAAGAAGCTCCTTATAGAAAAGTCAAAACAAGAGAAGATGGCGTGCAGAGATAAGAG
  CATGCAGGACCGATTGCGATTAGGCCACTTTACTACTGTCCGGCATGGAGCCTCGTTTACTGAGCAGTGG
  ACAGATGGTTATGCTTTCCAAAACCTCATCAAGCAACAGGAAAGGATAAATTCACAGAGAGAAGAGATAG
  AAAGGCAACGGAAAATGTTAGCAAAACGGAAACCTCCTGCCATGGGTCAGGCCCCTCCTGCAACCAATGA
  GCAGAAACAACGGAAAAGCAAGACTAATGGAGCTGAAAATGAAACGTTAACGTTAGCCGAGTACCATGAA
  CAAGAGGAAATCTTCAAACTTAGATTAGGTCATCTTAAGAAAGAGGAAGCAGAAATCCAGGCAGAGCTGG
  AAAGGCTGGAAAGGGTTAGGAATCTACACATCAGGGAATTAAAAAGGATACATAATGAAGACAATTCGCA
  GTTTAAAGACCATCCAACACTAAATGACAGATATTTGTTGTTACATCTTTTGGGTAGAGGAGGTTTCAGT
  GAAGTTTACAAGGCATTTGATCTAACGGAGCAAAGATATGTAGCTGTGAAAATTCACCAGTTAAATAAAA
  ACTGGAGAGATGAGAAAAAGGAGAATTACCACAAGCATGCGTGTAGGGAATACCGGATTCACAAGGAGCT
  GGACCACCCCAGGATAGTGAAGCTGTATGATTACTTTTCACTGGACACTGACTCGTTTTGTACAGTATTA
  GAATACTGTGAAGGGAATGACCTGGACTTCTACCTAAAACAGCACAAATTAATGTCGGAGAAAGAAGCCC
  GATCCATTATTATGCAGATTGTGAATGCTTTAAAGTACTTAAATGAAATAAAACCTCCCATCATACACTA
  TGACCTCAAACCAGGTAATATCCTTTTAGTAAATGGTACAGCATGTGGAGAGATAAAAATTACAGATTTT
  GGTCTTTCCAAGATCATGGATGATGATAGCTACAATTCAGTGGATGGCATGGAGCTGACGTCACAAGGAG
  CTGGTACTTATTGGTATTTACCACCAGAGTGTTTTGTGGTTGGGAAAGAGCCACCAAAGATCTCAAATAA
  AGTCGATGTTTGGTCAGTGGGTGTGATCTTCTACCAGTGTCTTTATGGGAGGAAGCCTTTTGGCCATAAC
  CAGTCCCAGCAAGATATTCTACAAGAGAATACTATTCTTAAGGCTACTGAAGTACAGTTCCCGCCAAAGC
  CAGTAGTAACACCTGAAGCAAAGGCATTTATCAGGAGATGTCTGGCCTATCGAAAGGAAGATCGCATTGA
  TGTGCAGCAGCTGGCCTGTGACCCCTACTTGTTGCCTCACATCCGAAAGTCAGTCTCCACAAGTAGCCCT
  GCAGGAGCTGCTATTGCATCAACCTCTGGGGCATCCAATAACAGTTCTTCGAATTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001294331
• Insert Size: 2157 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001294331.1, NP_001281260.1
• RefSeq Size: 5494 bp
• RefSeq ORF: 2157 bp
• Locus ID: 24086
• UniProt ID: O55047
• Cytogenetics: 11 E1
• Gene Summary: Serine/threonine-protein kinase involved in the process of chromatin assembly and probably also DNA replication, transcription, repair, and chromosome segregation. Phosphorylates the chromatin assembly factors ASF1A AND ASF1B. Phosphorylation of ASF1A prevents its proteasome-mediated degradation, thereby enhancing chromatin assembly (By similarity). Negative regulator of amino acid starvation-induced autophagy (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (B) differs in the 5' UTR and lacks an alternate in-frame exon compared to variant A. The encoded isoform (B) is shorter than isoform A. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.