Cdh8 (NM_001285914) Mouse Untagged Clone

CAT#: MC228741

Cdh8 (untagged) - Mouse cadherin 8 (Cdh8), transcript variant 4

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cdh8
• Synonyms: AI851472; cad; cad8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228741 representing NM_001285914
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCAGAAAGGCTAGCTGAGACGCTCATGGACCTCTGGACTCCATTAATAATATTATGGATTACCCTCC
  CTTCTTGTGTATACACGGCTCCGATGAATCAGGCTCACGTTTTAACTACTGGATCCCCTTTGGAACTAAG
  CAGGCAGAGTGAAGACATGCGGATTTTGAGCCGCTCCAAAAGAGGCTGGGTTTGGAATCAAATGTTTGTC
  CTGGAAGAATTTTCTGGACCTGAACCGATTCTCGTTGGCCGGTTACACACAGATCTGGATCCTGGAAGCA
  AAAAAATCAAGTATATCCTATCGGGTGATGGAGCTGGCACAATCTTTCAAATAAATGATATAACTGGAGA
  TATCCATGCTATCAAAAGACTTGACCGAGAGGAAAAGGCTGAATATACGTTAACGGCTCAGGCAGTGGAC
  TTTGAGACAAACAAGCCCCTTGAGCCTCCTTCTGAATTTATTATTAAGGTTCAAGATATCAACGACAATG
  CACCTGAGTTCCTCAATGGACCTTATCATGCTACTGTTCCAGAGATGTCCATTTTGGGTACATCTGTCAC
  TAATGTAACTGCCACTGATGCTGACGACCCAGTTTATGGAAACAGTGCAAAGTTGGTTTACAGTATCTTG
  GAGGGACAGCCGTATTTTTCCATTGAGCCTGAAACAGCCATTATAAAAACTGCCCTTCCTAACATGGACA
  GAGAAGCCAAGGAGGAATACCTGGTCGTAATACAAGCCAAAGATATGGGTGGACACTCTGGTGGTCTGTC
  TGGAACCACAACACTCACAGTGACCCTGACTGATGTTAATGACAATCCTCCAAAATTTGCTCAGAGTTTG
  TATCACTTCTCAGTACCAGAAGATGTGGTCCTTGGCACTGCGATAGGAAGGGTTAAGGCTAATGATCAGG
  ATATTGGTGAAAATGCACAATCATCCTATGACATCATTGATGGAGATGGGACAGCACTATTCGAAATCAC
  TTCTGATGCCCAGGCCCAGGATGGTGTTATAAGACTGAGAAAGCCTCTGGACTTTGAGACCAAAAAATCC
  TATACTCTGAAGGTGGAAGCAGCCAATATCCACATCGACCCACGTTTCAGTAGCAGGGGACCCTTTAAGG
  ATACAGCAACAGTCAAAATTGTCGTAGAGGATGCTGATGAGCCTCCGGTCTTCTCTTCACCAACTTACCT
  CCTTGAAGTTCATGAAAATGCTGCTTTGAACTCTGTGATTGGCCAAGTGACAGCTCGTGACCCTGATATC
  ACTTCCAGTCCAATAAGGTTTTCCATTGACCGCCACACTGACCTGGAAAGACAGTTCAACATCAATGCAG
  ATGATGGGAAGATAACACTGGCAACTCCACTGGACAGAGAACTAAGTGTTTGGCACAACATCACCATCAT
  TGCTACTGAGATCAGGAACCACAGTCAGATATCTCGAGTGCCTGTTGCTATTAAAGTGCTGGATGTCAAT
  GACAACGCCCCTGAATTCGCATCCGAATATGAGGCATTTTTATGTGAAAATGGAAAACCCGGCCAAGTCA
  TTCAAACAGTAAGTGCCATGGACAAAGACGATCCCAAAAACGGACATTTTTTCTTGTACAGTCTTCTTCC
  AGAAATGGTCAACAACCCAAATTTCACCATCAAGAAAAATGAAGATAATTCCCTGAGCATTCTGGCAAAA
  CATAATGGATTCAACCGCCAGAAGCAAGAAGTCTACCTTCTGCCTATAGTGATCAGTGACAGTGGAAACC
  CCCCTCTGAGTAGCACCAGTACGTTGACCATCCGAGTCTGTGGCTGTAGCAATGATGGCGTGGTCCAGTC
  GTGCAATGTTGAAGCTTATGTCCTTCCTATTGGACTCAGTATGGGCGCCTTAATTGCTATATTAGCATGC
  ATCATTTTGCTGCTCGTCATTGTGGTTCTGTTTGTTACCCTGAGGAGGCACAAAAATGAACCACTTATTA
  TCAAAGATGACGAAGACGTTCGAGAAAACATCATTCGCTACGACGACGAAGGAGGAGGGGAGGAGGACAC
  AGAGGCTTTTGACATTGCAACTTTGCAAAACCCAGATGGAATTAATGGATTTTTACCCCATTACCAAAGA
  GGCTTCCTAAGAGGAATACCAGAGAATGCTGCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001285914
• Insert Size: 2136 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001285914.1, NP_001272843.1
• RefSeq Size: 3166 bp
• RefSeq ORF: 2136 bp
• Locus ID: 12564
• Cytogenetics: 8 49.4 cM
• Gene Summary: This gene encodes a member of the cadherin family of calcium-dependent glycoproteins that mediate cell adhesion and regulate many morphogenetic events during development. The encoded preproprotein is further processed to generate a mature protein. Mice lacking the encoded protein exhibit reduced behavioral responses to cold, but not thermal stimuli. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing. Multiple distinct genes of the cadherin family, including this gene, are found on chromosome 8. [provided by RefSeq, Oct 2015]
  Transcript Variant: This variant (4) uses an alternate splice site in the 3' coding region and includes an alternate 3' terminal exon compared to variant 2. The resulting protein (isoform 4) is shorter and has a distinct C-terminus compared to isoform 2. This isoform (4) may undergo proteolytic processing similar to isoform 2.