Apbb1 (NM_001253886) Mouse Untagged Clone

CAT#: MC228737

Apbb1 (untagged) - Mouse amyloid beta (A4) precursor protein-binding, family B, member 1 (Apbb1), transcript variant 4

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Apbb1
• Synonyms: Fe65; Rir
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228737 representing NM_001253886
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGTTCCATCATCCCTGAGCCAGTCGGCCATTAACGCTAACAGCCACGGAGGCCCTGCACTCAGCT
  TCCCCTTACCCCTGCACGCTGCCCATAACCAGCTGCTCAACGCCAAGCTGCAAGCCACAGCTGTGGTACC
  CAAGGACCTTCGAAGTGCTATGGGAGAGGGTAGTGTGCCTGAACCAGGCCCTGCCAATGCCAAGTGGTTA
  AAGGAAGGCCAGAACCAGCTTCGGAGGGCTGCCACAGCCCACCGAGACCAGAACCGAAATGTGACCTTGA
  CCTTGGCGGAGGAGGCCAGCCAGGAGGCTGAGACGGCACCTTTGGGTCCCAAAGGCTTAATGCATCTATA
  CTCTGAGCTGGAGCTCTCGGCCCACAATGCAGCCAACCGAGGGCTTCATGGATCCGCCTTGATCATCAAC
  ACCCAGGAACAGGGACCAGATGAAGGAGAGGAGAAGGCAGCAGGAGAGGCTGAGGAGGATGATGAAGACG
  AAGAGGAGGAGGAGGAGGAGGAGGACTTGTCTTCTCCTCCAGGGCTACCTGAGCCTCTGGAGAATGTGGA
  AGTCCCCTCTGGGCCCCAGGCCCTCACAGACGGCCCCCGGGAACACAGCAAGAGTGCTAGCCTCCTATTT
  GGCATGCGAAACAGTGCAGCCAGTGATGAGGACTCAAGCTGGGCCACCTTATCACAGGGCAGCCCCTCCT
  ATGGCTCCCCGGAGGACACAGATTCCTTCTGGAACCCCAACGCTTTCGAGACGGATTCCGATCTACCGGC
  TGGATGGATGAGGGTACAGGACACCTCAGGGACCTACTACTGGCACATCCCAACAGGGACCACCCAGTGG
  GAACCCCCAGGCCGGGCCTCCCCCTCACAGGGGAGCAGCCCCCAAGAAGAGTCCCAGCTCACCTGGACTG
  GCTTTGCTCACCAAGAAGGCTTTGAGGAAGGAGAGTTTTGGAAGGATGAACCCAGTGAGGAGGCCCCAAT
  GGAGTTGGGACTGAAGGACCCCGAGGAGGCGACATTGTCCTTCCCAGCTCAGAGCCTCAGCCCAGAACCA
  GTTCCCCAGGAGGAAGAGAAGCTGTCCCAACGGAATGCCAACCCAGGGATCAAGTGTTTCGCTGTGCGCT
  CCCTAGGCTGGGTAGAGATGACCGAGGAGGAGCTGGCCCCAGGACGCAGCAGTGTGGCAGTCAACAATTG
  TATCCGCCAGCTCTCCTACCACAAAAACAATCTACATGATCCGATGGCTGGGGGCTGGGGAGAGGGAAAG
  GATCTGCTGCTCCAGCTGGAGGACGAGACTCTAAAGTTGGTGGAGCCACAGAACCAGACGCTGCTGCATG
  CACAGCCCATCGTCAGCATTCGTGTGTGGGGCGTTGGGCGGGACAGTGGAAGGGACTTTGCCTACGTAGC
  TCGAGATAAGCTGACCCAGATGCTCAAGTGCCACGTGTTTCGCTGTGAGGCACCTGCCAAGAACATCGCC
  ACCAGCCTGCATGAGATCTGCTCCAAGATCATGTCTGAACGGCGCAATGCTCGCTGCTTGGTCAATGGAC
  TCTCCCTAGACCACTCTAAACTCGTGGATGTCCCTTTCCAAGTGGAATTCCCAGCACCAAAGAATGAGCT
  GGTGCAGAAGTTCCAAGTCTATTACCTGGGAAATGTGCCAGTTGCTAAACCTGTTGGGGTAGACGTGATT
  AATGGGGCCCTGGAGTCAGTCCTGTCTTCCAGTAGCCGTGAGCAGTGGACTCCAAGTCACGTCAGCGTGG
  CCCCTGCCACCCTCACCATCTTGCACCAGCAGACAGAAGCGGTGCTGGGGGAGTGCCGGGTGCGGTTTCT
  CTCCTTCCTGGCTGTGGGCAGAGATGTGCACACATTCGCGTTCATCATGGCTGCCGGCCCAGCCTCCTTC
  TGCTGTCACATGTTTTGGTGTGAGCCCAATGCTGCCAGTCTCTCAGAGGCTGTGCAGGCTGCATGCATGC
  TCCGCTACCAGAAGTGTCTGGATGCTCGCTCCCAGACCTCCACCTCCTGCCTCCCAGCACCCCCTGCGGA
  GTCAGTTGCAAGACGTGTAGGGTGGACAGTCCGCAGGGGTGTTCAGTCGCTGTGGGGTTCCCTCAAGCCC
  AAACGTCTGGGATCCCAGACCCCATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001253886
• Insert Size: 2127 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001253886.1, NP_001240815.1
• RefSeq Size: 2916 bp
• RefSeq ORF: 2127 bp
• Locus ID: 11785
• UniProt ID: Q9QXJ1
• Cytogenetics: 7 55.9 cM
• Gene Summary: Adapter protein that forms a transcriptionally active complex with the gamma-secretase-derived amyloid precursor protein (APP) intracellular domain. Plays a central role in the response to DNA damage by translocating to the nucleus and inducing apoptosis. May act by specifically recognizing and binding histone H2AX phosphorylated on 'Tyr-142' (H2AXY142ph) at double-strand breaks (DSBs), recruiting other pro-apoptosis factors such as MAPK8/JNK1. Required for histone H4 acetylation at double-strand breaks (DSBs). Its ability to specifically bind modified histones and chromatin modifying enzymes such as KAT5/TIP60, probably explains its transcription activation activity. Function in association with TSHZ3, SET and HDAC factors as a transcriptional repressor, that inhibits the expression of CASP4. Associates with chromatin in a region surrounding the CASP4 transcriptional start site(s).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (4) lacks an in-frame exon in the coding region compared to variant 1. This results in a shorter protein (isoform 2) compared to isoform 1. Variants 2 and 4 encode the same isoform (2). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.