Nfatc2 (NM_001291177) Mouse Untagged Clone

CAT#: MC228731

Nfatc2 (untagged) - Mouse nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 2 (Nfatc2), transcript variant 14

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nfatc2
• Synonyms: AI607462; NF-ATc2; NF-ATp; NFAT1; NFAT1-D; Nfatp
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228731 representing NM_001291177
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCACCTCGAACCAGCCTCGCCGAGGACAGCTGCCTGGGCCGACACTCGCCCGTGCCCCGTCCGGCAT
  CCCGCTCCTCCTCACCCGGTGCCAAGCGGAGGCATTCGTGCGCAGAGGCTTTGGTTGCTCCTCTGCCCGC
  AGCCTCACCCCAGCGCTCCCGGAGCCCCTCGCCACAGCCCTCGCCTCACGTGGCACTGCAGGACGACAGC
  ATCCCCGCTGGGTACCCCCCCACGGCCGGCTCTGCTGTTCTCATGGATGCCCTCAACACCCTGGCCACCG
  ACTCGCCCTGCGGGATCCCCTCCAAGATATGGAAGACCAGTCCTGACCCGACGCCTGTGTCCACCGCTCC
  GTCCAAGGCTGGCCTGGCCCGCCACATCTACCCTACTGTGGAGTTCCTGGGGCCATGTGAGCAGGAGGAG
  AGGAGGAATTCCGCTCCAGAGTCCATCCTGCTGGTACCACCTACTTGGCCCAAGCAGTTGGTGCCGGCCA
  TTCCCATCTGCAGCATCCCTGTGACTGCATCCCTCCCACCACTCGAGTGGCCACTCTCCAATCAGTCGGG
  CTCCTATGAGCTACGGATTGAGGTCCAACCCAAGCCCCATCACCGGGCCCACTATGAGACGGAGGGCAGC
  CGTGGCGCTGTCAAAGCCCCAACAGGAGGACACCCTGTGGTGCAGCTCCACGGCTACATGGAGAACAAGC
  CTCTGGGGCTTCAGATCTTCATTGGGACAGCAGATGAGAGGATCCTTAAGCCGCACGCCTTCTACCAAGT
  ACACAGGATCACTGGGAAAACGGTCACCACCACGAGCTATGAGAAGATCGTAGGCAACACCAAGGTCCTG
  GAGATCCCCCTGGAGCCAAAGAACAACATGAGAGCCACCATCGACTGTGCAGGCATCCTGAAGCTCCGAA
  ACGCTGACATCGAGCTGCGGAAGGGCGAGACGGACATCGGCAGGAAGAACACGCGTGTGCGCCTGGTGTT
  CCGCGTGCACGTCCCAGAGCCCAGTGGGCGCATCGTCTCCCTGCAGGCTGCGTCCAACCCCATCGAGTGC
  TCTCAGCGCTCTGCCCACGAGCTGCCCATGGTGGAGAGACAAGACATGGACAGCTGCCTGGTCTACGGGG
  GCCAGCAGATGATCCTCACGGGCCAGAACTTCACAGCGGAGTCCAAGGTTGTGTTCATGGAGAAGACTAC
  AGATGGGCAGCAGATTTGGGAGATGGAAGCTACGGTGGATAAAGACAAGAGCCAGCCTAACATGCTTTTT
  GTTGAGATCCCCGAGTATCGGAACAAGCACATCCGCGTGCCCGTGAAAGTCAACTTCTACGTCATCAACG
  GAAAGAGGAAACGAAGTCAGCCACAGCACTTTACCTACCACCCAGTCCCTGCCATCAAGACAGAGCCCAG
  CGATGAGTATGAACCATCTTTGATCTGCAGCCCCGCCCATGGAGGCCTGGGGAGCCAGCCATATTACCCA
  CAGCACCCAATGCTGGCCGAGTCCCCCTCCTGCCTTGTGGCTACCATGGCCCCCTGCCAACAGTTCCGCT
  CGGGGCTCTCATCCCCCGATGCTCGCTACCAACAGCAGAGCCCCGCAGCTGCCCTCTACCAGAGAAGCAA
  GAGCCTGAGTCCCGGCCTGCTGGGCTACCAGCAGCCGTCCCTCCTGGCAGCACCCTTGGGTCTGGCTGAT
  GCCCACCGCTCTGTGCTGGTGCATGCTGGTTCTCAGGGGCAGGGGCAGGGCTCCACCCTGCCACACACAT
  CCTCGGCCAGCCAGCAGGCCTCACCCGTGATCCACTACTCACCCACCAACCAGCAGCTTCGCGGTGGGGG
  TCACCAGGAGTTCCAGCATATCATGTACTGTGAAAACTTCGGCCCCAGCTCTGCCAGGCCTGGCCCGCCT
  CCCATCAACCAAGGTCAGAGGCTGAGCCCGGGCGCCTACCCCACAGTCATCCAACAACAGACTGCCCCGA
  GCCAAAGAGCTGCCAAAAACGGACCCAGTGACCAGAAGGAAGCTCTGCCCACGGGAGTGACCGTCAAACA
  GGAACAGAACCTGGACCAGACCTACCTGGATGACGTTAATGAAATCATCAGGAAGGAGTTTTCAGGACCT
  CCCTCCCGAAATCAGACCTAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_001291177
• Insert Size: 2121 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001291177.1, NP_001278106.1
• RefSeq Size: 6239 bp
• RefSeq ORF: 2121 bp
• Locus ID: 18019
• Cytogenetics: 2 88.91 cM
• Gene Summary: Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2, IL-3, IL-4, TNF-alpha or GM-CSF. Promotes invasive migration through the activation of GPC6 expression and WNT5A signaling pathway.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (14, also known as IB-IIS-deltaXa) contains alternate 5' exon structure, and it thus differs in the 5' UTR and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (g) is shorter at the N-terminus, compared to isoform a. Both variants 7 and 14 encode isoform g.