Mapk7 (NM_001291037) Mouse Untagged Clone

CAT#: MC228722

Mapk7 (untagged) - Mouse mitogen-activated protein kinase 7 (Mapk7), transcript variant 6

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mapk7
• Synonyms: BMK-1; BMK1; ERK-5; ERK5; Erk5-T; PRKM7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228722 representing NM_001291037
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCTTTTCCTGCTCCCCAGGCCAGCAGGTGGCCATCAAGAAGATACCTAATGCTTTTGATGTGGTGA
  CCAATGCCAAACGGACCCTCAGGGAGCTGAAGATCCTCAAACACTTCAAACACGACAATATCATCGCCAT
  CAAGGACATCCTGAAGCCTACTGTGCCCTATGGAGAATTCAGATCTGTCTATGTGGTACTGGACCTCATG
  GAGAGCGACCTACACCAGATCATTCACTCTTCACAGCCGCTCACCCTGGAACATGTGAGATACTTCCTGT
  ACCAGCTGCTTCGGGGCCTCAAATACATGCACTCTGCTCAGGTCATCCACCGTGATCTTAAACCCTCTAA
  CCTTCTGGTCAATGAGAACTGTGAGCTCAAGATCGGTGACTTTGGAATGGCCCGTGGCCTCTGTACTTCC
  CCTGCCGAGCACCAGTACTTCATGACTGAGTATGTGGCTACTCGCTGGTACCGTGCCCCGGAGCTCATGC
  TTTCCCTGCACGAGTATACGCAGGCAATCGACCTCTGGTCTGTGGGCTGCATCTTTGGTGAGATGCTGGC
  TCGGCGCCAGCTCTTCCCAGGCAAAAACTACGTGCACCAGTTACAGCTGATCATGATGGTGTTGGGAACT
  CCGTCACCAGCTGTGATTCAGGCTGTGGGGGCTGAAAGGGTGCGAGCCTATATCCAGAGCCTGCCACCAA
  GGCAACCTGTGCCTTGGGAGACAGTATACCCAGGTGCTGACCGCCAGGCCCTCTCCCTGCTGGGACGCAT
  GTTGCGATTTGAACCCAGTGCCCGAATCTCAGCTGCTGCTGCCCTTCGCCACCCCTTCCTGGCTAAGTAC
  CATGACCCTGATGATGAGCCTGATTGCGCCCCACCTTTTGACTTTGCTTTTGACCGTGAAGCCCTTACCA
  GGGAGCGCATTAAGGAGGCCATTGTGGCTGAGATTGAGGACTTCCATGCACGACGGGAGGGCATCCGCCA
  ACAAATCCGCTTCCAGCCTTCTCTGCAGCCTGTGGCTAGTGAGCCTGTGTGTCCAGATGTTGAGATGCCC
  AGTCCCTGGGCTCCAAGTGGAGACTGTGCCATGGAGTCGCCTCCTCCAGCACTGCCACCATGCTCTGATC
  CTGCACCTGACACCGTTGATCTGACTCTGCAGCCTGCCCCGCCGGCCAATGGGCCCAGTGCACCCTTGGA
  GGCGCCTGAGCCTCGAAAGCCCGTGACAGCTCAGGAACGCCAGCGAGAACGAGAAGAGAAGCGCAGGAGG
  CGACAAGAGAGAGCCAAGGAGCGGGAGAAGCGACGACAAGAGAGAGAACGCAAGGAGAGGGGGGCTGGTA
  CCTTGGGGGGCCCCTCTACTGACCCTCTGGCTGGACTGGTGCTCAGTGACAATGACCGAAGCCTGCTAGA
  GCGGTGGACTCGCATGGCTAGGCCTCCTGCCCCTGCCCCTGCCCCAGCGCCAGCACCAGCGCCAGCACCG
  TCCTCTGCCCAGCCCACTAGTACTCCTACTGGCCCCGTATCTCAGTCTACTGGTCCTCTACAGCCTGCAG
  GCTCTATTCCGGGTCCTGCCTCCCAGCCTGTTTGCCCACCCCCTGGCCCTGTTCCCCAGCCTGCTGGCCC
  TATCCCTGCTCCGCTCCAGACTGCCCCTTCCACTAGCCTTTTGGCCTCCCAGTCACTTGTGCCACCTAGT
  GGGTTGCCTGGTTCTGGTGCCCCAGAAGTTCTGCCTTACTTCCCATCTGGCCCACCACCTCCAGATCCTG
  GGCTCACCCCTCAGCCTTCTACATCAGAGTCACCTGATGTCAACCTGGTGACTCAGCAGCTGTCCAAGTC
  TCAGGTGGAGGACCCCCTGCCTCCTGTGTTCTCTGGCACTCCAAAGGGCAGTGGGGCTGGCTACGGAGTT
  GGCTTTGATCTGGAGGAATTCTTAAATCAATCTTTTGATATGGGTGTGGCTGATGGGCCACAGGATGGCC
  AGGCAGACTCAGCCTCACTCTCAGCCTCTCTCCTTGCTGACTGGCTTGAGGGCCATGGCATGAACCCTGC
  TGACATTGAGTCTCTGCAGCGTGAGATCCAGATGGACTCCCCAATGCTGCTGTCTGACCTGCCTGACCTC
  CAAGAGCCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001291037
• Insert Size: 2112 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001291037.1, NP_001277966.1
• RefSeq Size: 3264 bp
• RefSeq ORF: 2112 bp
• Locus ID: 23939
• UniProt ID: Q9WVS8
• Cytogenetics: 11 B2
• Gene Summary: Plays a role in various cellular processes such as proliferation, differentiation and cell survival. The upstream activator of MAPK7 is the MAPK kinase MAP2K5. Upon activation, it translocates to the nucleus and phosphorylates various downstream targets including MEF2C. EGF activates MAPK7 through a Ras-independent and MAP2K5-dependent pathway. May have a role in muscle cell differentiation. May be important for endothelial function and maintenance of blood vessel integrity. MAP2K5 and MAPK7 interact specifically with one another and not with MEK1/ERK1 or MEK2/ERK2 pathways. Phosphorylates SGK1 at Ser-78 and this is required for growth factor-induced cell cycle progression (By similarity). Involved in the regulation of p53/TP53 by disrupting the PML-MDM2 interaction (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (6) differs in its 5' UTR, uses an alternate start codon, and uses an alternate splice site in the central coding region, compared to variant 1. The encoded isoform (d) is shorter and has a distinct N-terminus, compared to isoform a.