Adarb2 (NM_001289530) Mouse Untagged Clone

CAT#: MC228717

Adarb2 (untagged) - Mouse adenosine deaminase, RNA-specific, B2 (Adarb2), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Adarb2
• Synonyms: Adar3; RED2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228717 representing NM_001289530
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCATCTGTCCTGGGGAGTGGCAGAGGGTCTGGAGGGCTAAGCAGTCAACTCAAATGCAAGTCCAAGA
  GGAGGAGGAGGAGGAGGTCCAAGAGGAAAGACAAAGTCAGCATACTGTCAACCTTCCTTGCTCCTTTCAA
  GTACCTAAGTCCTGGCACCACAAACACAGAGGATGAAGATAACCTAAGTACCAGCAGTGCTGAAGTGAAG
  GAAAACCGAAATGTGAGCAACCTGGGGACCAGGCCCCTGCCCCCTGGAGACTGGGCCAGAGGCAGCACAC
  CCAGCGTTAAAAGGAAAAGGCCGCTGGAGGAAGGAAATGGGGGACATTTCTGCAAACTCCAGCTGATCTG
  GAAGAAGCTCTCCTGGTCTGTGACACCCAAGAATGCGCTGGTCCAGCTGCATGAGCTGAAGCCTGGTCTG
  CAGTACCGAATGGTGTCACAGACGGGCCCGGTGCACGCCCCAGTTTTCGCAGTGGCTGTAGAAGTGAACG
  GCCTCACATTTGAAGGCACGGGGCCCACTAAGAAGAAGGCCAAGATGCGGGCAGCAGAAATGGCCCTGAA
  GTCCTTTGTGCAGTTCCCCAATGCCTTCCAGGCCCACCTGGCCATGGGCAGTAGTACCAGCCCGTGCACT
  GACTTCACCTCTGACCAGGCTGACTTCCCAGACACGTTGTTCAAGGAGTTTGAACCATCCTCAAAAAACG
  AAGACTTCCCAGGCTGTCATCCCGTTGATACTGAGTTTCTGTCCTCTGCCTATCGGCGAGGACGGCTACT
  CTACCACACCCTGGACCTCATGGGCCAGGCTCTGCCTGACAGGTCCAGGCTGGCTCCGGGAGCCTTGGGT
  GAGAGGAACCCTGTAGTGGTACTGAATGAGTTACGCTCAGGTCTTCGGTATGTGTGCCTCTCCGAGACAG
  CCGAGAAGCCCCGAGTCAAGAGCTTTGTCATGGCTGTGTGCGTGGATGGGAGGACGTTCGAAGGCTCTGG
  ACGCAGCAAGAAGCTGGCCAAGGGCCAAGCAGCACAAGCTGCCTTGCAGGCCCTCTTCGATATCCGGCTG
  CCTGGTCACATACCCAGCAGGAGTAAAAGCAACCTCTTGCCACAGGATTTTGCAGACTCTGTGTCCCAGC
  TGGTCACACAGAAGTTCCGTGAACTGACAGTCGGCCTGACATCTGTGTATGCCCGCCACAAAACACTGGC
  AGGAATCGTCATGACCAAAGGCTTGGACACCAAACAAGCGCAGGTCATCGTTCTGTCCTCTGGGACCAAG
  TGTATCAGCGGTGAGCACATCAGTGACCAGGGGCTGGTTGTCAACGACTGTCATGCTGAGATCGTGGCGA
  GGCGGGCATTTCTTCACTTCCTCTACTCTCAGCTGGAGCTGCACTTGAGCAAGCATCAAGAGGACCCCGA
  GAGATCAATATTCATACGTTTAAAGGAAGGAGGTTACAGGCTGCGAGAAAACATCCTCTTCCATCTCTAC
  GTGAGCACATCCCCCTGTGGAGATGCCAGAGTCAACTCTCCATATGAAATCACCACAGACTTGAACAGCA
  GCAAACACATCGTCAGGAAGTTCCGAGGGCACCTGCGCACCAAGATTGAGTCTGGGGAAGGAACAGTCCC
  TGTGCGGGGCCCCAGTGCAGTCCAGACGTGGGATGGCATCCTATTGGGGGAGCAGCTAATCACCATGTCC
  TGCACAGACAAGATTGCCAGCTGGAATGTGCTGGGACTACAAGGCGCTCTCCTCTGTCACTTCATCGAGC
  CTGTGTACCTCCACAGCATCATTGTGGGCAGCCTGCACCACACGGGCCATCTTGCACGGGTCATGAGCCA
  CAGGATGGAGGGAATCGGCCAGCTGCCTGCCTCATACCGACAAAACAGGCCTCTCCTTAGTGGCGTGAGT
  CACGCAGAGGCCCGGCAGCCAGGAAAGTCTCCCCACTTCAGTGCCAACTGGGTCGTGGGCAGCGCCGACC
  TGGAGATCATTAATGCCACCACTGGGAAGAGGAGCTGTGGGGGCTCATCCCGGCTCTGCAAACACGTGTT
  TTCTGCTTGGTGGGCACGGCTGCATGCTAAGCACACGGATCCCCAGCCATGGAGACACACCATCCATGTA
  CTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001289530
• Insert Size: 2106 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289530.1, NP_001276459.1
• RefSeq Size: 5628 bp
• RefSeq ORF: 2106 bp
• Locus ID: 94191
• Cytogenetics: 13 A1
• Gene Summary: Lacks editing activity. It prevents the binding of other ADAR enzymes to targets in vitro, and decreases the efficiency of these enzymes. Capable of binding to dsRNA but also to ssRNA (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses an alternate splice site in the 3' coding region, compared to variant 1, which results in a frameshift and a protein (isoform 2) with a shorter and distinct C-terminus compared to isoform 1.