Adam5 (NM_001272057) Mouse Untagged Clone

CAT#: MC228716

Adam5 (untagged) - Mouse a disintegrin and metallopeptidase domain 5 (Adam5), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Adam5
• Synonyms: tMDC; tMDC II; tMDCII
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228716 representing NM_001272057
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCCTCCTTCTTTTGCTCTTTTTGCATCTTAAAGGGCTGCAGGCAGGCCAGAATCCTCAGAAAACCA
  CCCTGCAGACAACTGTTCCTGAAAAGATCTCATCACCGGATGTGGAAACAGATGCAGAAGACCATATGGC
  TTATCTTATTACAATAAATGAAACCCCCCATTTCATCCATCTTAAAAAACAATCATTTATAACTCCAACT
  GCAGTTGTTTATACTTACGACAGAAATGATGTTCAACATTCTCAACCTTTGTCAGCTCTGGAAAATTGCA
  ATTACAATGGATATGTTGCAGGTTTCCCAAACTCTATCGTGACCCTCACCGTTTGCACAGGACTCAGGGG
  AATCATACAGTTTGAAAATGTCTCCTATGCAATCGAGCCCGTGGAAACCCTGTCAGGATTTGTGCACGTG
  ATCTATGAGAACACAAACAAACATGCTGTGATTCCTGACCTGGGAAAGAATCAGTCATACAGTTGGTTTG
  ACGAATCAGATTATCAATTCCGGAGTAACATGAAGAAATCTGGATTTACCGTGTTACGCCAACGATTCAT
  TATGATGGATATCATCGTGGATAAAAAGCTGTTTGATTACATGGGTTCTGACACTGAAGTTGTGCTACAG
  AAAGTCATTCAGATCATCGGTTTCGTTAACACTATGCTCTCCAAGTTAAAACTGACTGTCTTAATAAATT
  CCATTGAAATCTGGTCGAAGGAAAACAGAATTCGTTTATCAAAGGCAGTTGATGACTTATTGGTTCAGTT
  CTCAATTTGGAAACATGAGTATAGATCACAACATGTTGCATACTTACTGGCATTTGAGGAGCACCCTGCT
  TCCACGGGGGCTCTATATCCAGGGAATCTGTGCAAACTGGAGTACAATGCTGCTGTTGCCCTGTATCCAA
  AAGGTTTATCGTTAGAATCGTTTAGTGTCATTGTCCTGCAGCTGCTGAGCATTGGTATGGGCTTAACTTA
  CGACACTGAGAACTGCCACTGTACAGGAGAAGTTTGCCTAATGACGCCCAAAGCAATATATTCTGGGGGT
  GTGAAGGACTTCAGTACTTGTACCCTGGATGACTTTAAATACCTGTCAACAAGACAAGACCTTAGATGTC
  TCCAGGACTTGCCTTTGGAAAGAAAGCCAGCTCGCAGGCCAAGGAGAATCTGTGGCAATGGGATACTGGA
  GATGAACGAGCAATGTGACTGTGGCACTTTGAAGAACTGTACACATAGAAAATGCTGTGACCCTATGTCA
  TGTCGATTGAAAAACAAGGCCACGTGTGGTTCTGGGGAGTGCTGTAGTCAAGATTGCACGGTAAAAATGA
  ATGATGTGGTCTGTAGAAAGTCGGTAGATGAATGTGACTTTGTGGAGTACTGTAACGGAAAAGATCCCTA
  TTGTGTCCCTAACACATATGCACGCAATGGGCAGTATTGTGAATCAGGTGAAGCCTTCTGTTTTGAAGGA
  CGATGTCAGACTGCTGACAAGCAATGTATGAGCATGCTTGGGAAATACGTGAGAGGGGCTTCCTTTGCGT
  GCTATGAAGAGTTCAATTCCAGAGGCGATAGATTTGGCAACTGTATTCATAATTTTTGTGCTTTCAGAAA
  TTCTCTCTGTGGGAAACTAATCTGTACGTGGCCATTCAAGAAGCTGGTGCTAAAAGCCAATTTATCTGTG
  GCCTATGCGCAAATCCGGGATGACTTGTGTGTAGCTATGTATAAAGGTGGGAGGATACCTAAGACAACAA
  AGACGACATATTCCAATCCAGCGGACAGAGATGAGACATTTGTAAACGACGGCACCATCTGTGGCCCTGA
  TATGTTTTGTTTGAGAGCATCATGCACAGAAACTAGATTTCACATGGATTCTTCCAAGTGTGATTCAACT
  AGAGATTGCAACGACCATGGGGTTTGCAACAATTTACAGCATTGCCACTGTGATATAGGATATAACCCTC
  CTTTTTGTGAGGAACATAAGGGACAGTTTGGAAGCGTTGATGATGGACATAAATATCATGTTGAAGCACC
  TCCCACTAATCAAGTATTGCTACCCCAAAAGAAGATAATCTGGATTAACCATGAGAAACTGACATTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001272057
• Insert Size: 2100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001272057.1, NP_001258986.1
• RefSeq Size: 2593 bp
• RefSeq ORF: 2100 bp
• Locus ID: 11499
• UniProt ID: Q3TTE0
• Cytogenetics: 8 13.26 cM
• Gene Summary: This gene encodes a member of a disintegrin and metalloprotease (ADAM) family of endoproteases that play important roles in various biological processes including cell signaling, adhesion and migration. The encoded preproprotein undergoes proteolytic processing to generate a mature, functional protein. This gene is located in a cluster of related ADAM genes on chromosome 8. Alternative splicing results in multiple transcript variants encoding different isoforms, some of which may undergo similar processing. [provided by RefSeq, May 2016]
  Transcript Variant: This variant (2) lacks several exons and contains an alternate 3' terminal exon, compared to variant 1. The encoded isoform (2) is shorter and has a distinct C-terminus, compared to isoform 1. This isoform (2) may undergo proteolytic processing similar to isoform 3.