Tlk2 (NM_001294334) Mouse Untagged Clone

CAT#: MC228707

Tlk2 (untagged) - Mouse tousled-like kinase 2 (Arabidopsis) (Tlk2), transcript variant E

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tlk2
• Synonyms: 4933403M19Rik; PKU-alpha; PKUalpha; Tlk
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228707 representing NM_001294334
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGGAAGAACTGCATAGCCTGGACCCACGAAGGCAGGAATTACTAGAGGCCAGGTTCACTGGAGTTG
  GTGTAAGTAAGGGGCCACTCAACAGTGAGTCTTCCAACCAGAGTCTGTGCAGCGTGGGGTCGTTGAGTGA
  TAAAGAAGTAGAGACTCCTGAGAAAAAGCAGAATGACCAGCGAAATCGGAAAAGGAAAGCCGAGCCATAT
  GACACTAGCCAAGGGAAAGGCACTCCTAGGGGACATAAAATTAGTGATTACTTTGAGTTTGCTGGGGGAA
  GCGGGCCAGGAACCAGCCCTGGCAGAAGTGTTCCACCAGTTGCACGATCCTCACCGCAACATTCCTTATC
  CAATCCCTTACCGCGTCGAGCAGAACAGCCTCTGTATGGTTTAGATGGCAGTGCAGCAAAGGAGGCCTCA
  GAAGAGCAGTCTGCTCTGCCCACCCTCATGTCAGTGATGTTAGCAAAACCGCGACTTGACACAGAGCAGT
  TAGCGCCACGGGGAGCTGGCCTCTGCTTCACTTTCGTCTCTGCTCAACAAAACAGTCCTTCTTCTACGGG
  TTCTGGCAATACAGAACATTCTTGCAGCTCCCAAAAACAGATCTCCATCCAGCACAGACAGACCCAGTCT
  GACCTCACAATAGAAAAAATATCTGCACTAGAAAACAGTAAGAACTCTGACTTAGAGAAGAAGGAAGGAA
  GAATAGATGATTTATTAAGAGCCAACTGTGATTTGAGACGACAGATAGATGAACAGCAAAAGATGCTAGA
  GAAATACAAGGAACGATTAAATAGATGTGTCACCATGAGCAAGAAGCTCCTTATAGAAAAGTCAAAACAA
  GAGAAGATGGCGTGCAGAGATAAGAGCATGCAGGACCGATTGCGATTAGGCCACTTTACTACTGTCCGGC
  ATGGAGCCTCGTTTACTGAGCAGTGGACAGATGGTTATGCTTTCCAAAACCTCATCAAGCAACAGGAAAG
  GATAAATTCACAGAGAGAAGAGATAGAAAGGCAACGGAAAATGTTAGCAAAACGGAAACCTCCTGCCATG
  GGTCAGGCCCCTCCTGCAACCAATGAGCAGAAACAACGGAAAAGCAAGACTAATGGAGCTGAAAATGAAA
  CGTTAACGTTAGCCGAGTACCATGAACAAGAGGAAATCTTCAAACTTAGATTAGGTCATCTTAAGAAAGA
  GGAAGCAGAAATCCAGGCAGAGCTGGAAAGGCTGGAAAGGGTTAGGAATCTACACATCAGGGAATTAAAA
  AGGATACATAATGAAGACAATTCGCAGTTTAAAGACCATCCAACACTAAATGACAGATATTTGTTGTTAC
  ATCTTTTGGGTAGAGGAGGTTTCAGTGAAGTTTACAAGGCATTTGATCTAACGGAGCAAAGATATGTAGC
  TGTGAAAATTCACCAGTTAAATAAAAACTGGAGAGATGAGAAAAAGGAGAATTACCACAAGCATGCGTGT
  AGGGAATACCGGATTCACAAGGAGCTGGACCACCCCAGGATAGTGAAGCTGTATGATTACTTTTCACTGG
  ACACTGACTCGTTTTGTACAGTATTAGAATACTGTGAAGGGAATGACCTGGACTTCTACCTAAAACAGCA
  CAAATTAATGTCGGAGAAAGAAGCCCGATCCATTATTATGCAGATTGTGAATGCTTTAAAGTACTTAAAT
  GAAATAAAACCTCCCATCATACACTATGACCTCAAACCAGGTAATATCCTTTTAGTAAATGGTACAGCAT
  GTGGAGAGATAAAAATTACAGATTTTGGTCTTTCCAAGATCATGGATGATGATAGCTACAATTCAGTGGA
  TGGCATGGAGCTGACGTCACAAGGAGCTGGTACTTATTGGTATTTACCACCAGAGTGTTTTGTGGTTGGG
  AAAGAGCCACCAAAGATCTCAAATAAAGTCGATGTTTGGTCAGTGGGTGTGATCTTCTACCAGTGTCTTT
  ATGGGAGGAAGGTGAGGAAGAAGGCAGTGGAGCAGCCTGGGGCCATACCCACCACCTCACTGGGATTCTT
  CTTTGCAGTTAATCATTGGAATCCTGAAAGTAGTGGTTTAAAGAAAATACAGACTTCTCAGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001294334
• Insert Size: 2094 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001294334.1, NP_001281263.1
• RefSeq Size: 3442 bp
• RefSeq ORF: 2094 bp
• Locus ID: 24086
• UniProt ID: O55047
• Cytogenetics: 11 E1
• Gene Summary: Serine/threonine-protein kinase involved in the process of chromatin assembly and probably also DNA replication, transcription, repair, and chromosome segregation. Phosphorylates the chromatin assembly factors ASF1A AND ASF1B. Phosphorylation of ASF1A prevents its proteasome-mediated degradation, thereby enhancing chromatin assembly (By similarity). Negative regulator of amino acid starvation-induced autophagy (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (E) contains a 3' terminal exon that extends past a splice site that is used in variant A. This results in a novel 3' coding region and 3' UTR compared to variant A. The encoded isoform (E) is shorter, and has a distinct C-terminus, compared to isoform A. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.